Imperial College London

Prof. Ramon Vilar

Faculty of Natural SciencesDepartment of Chemistry

Professor of Medicinal Inorganic Chemistry



+44 (0)20 7594 1967r.vilar Website




301HMolecular Sciences Research HubWhite City Campus






BibTex format

author = {Paulose, Nadappuram B and Cadinu, P and Barik, A and Ainscough, A and Devine, M and Kang, M and Gonzalez-Garcia, J and Kittler, J and Willison, K and Vilar, Compte R and Actis, P and Wojciak-Stothard, B and Oh, S-H and Ivanov, A and Edel, JB},
doi = {10.1038/s41565-018-0315-8},
journal = {Nature Nanotechnology},
pages = {80--88},
title = {Nanoscale tweezers for single cell biopsies},
url = {},
volume = {14},
year = {2019}

RIS format (EndNote, RefMan)

AB - Much of the functionality of multi-cellular systems arises from the spatial organisation and dynamic behaviours within and between cells. Current single-cell genomic methods only provide a transcriptional “snapshot” of individual cells. The real-time analysis and perturbation of living cells would generate a step-change in single-cellanalysis. Here we describe minimally invasive nanotweezers that can be spatially controlled to extract samples from living cells with single-molecule precision. They consist of two closely spaced electrodes with gaps as small as 10-20 nm, which can be usedfor the dielectrophoretic trapping of DNA and proteins.Aside from trapping single molecules, we also extract nucleic acids for gene expression analysis from living cells, without affecting their viability. Finally, we report on the trapping, and extraction of a single mitochondrion. This work bridges the gap between single-molecule/organelle manipulation and cell biology and can ultimately enable a better understanding of living cells.
AU - Paulose,Nadappuram B
AU - Cadinu,P
AU - Barik,A
AU - Ainscough,A
AU - Devine,M
AU - Kang,M
AU - Gonzalez-Garcia,J
AU - Kittler,J
AU - Willison,K
AU - Vilar,Compte R
AU - Actis,P
AU - Wojciak-Stothard,B
AU - Oh,S-H
AU - Ivanov,A
AU - Edel,JB
DO - 10.1038/s41565-018-0315-8
EP - 88
PY - 2019///
SN - 1748-3387
SP - 80
TI - Nanoscale tweezers for single cell biopsies
T2 - Nature Nanotechnology
UR -
UR -
VL - 14
ER -