Imperial College London

Prof. Ramon Vilar

Faculty of Natural SciencesDepartment of Chemistry

Professor of Medicinal Inorganic Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 1967r.vilar Website

 
 
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Location

 

301HMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cilibrizzi:2017:10.1016/j.snb.2017.03.171,
author = {Cilibrizzi, A and Terenghi, M and Fedorova, M and Woscholski, R and Klug, D and Vilar, R},
doi = {10.1016/j.snb.2017.03.171},
journal = {Sensors and Actuators B: Chemical},
pages = {437--446},
title = {Small-molecule optical probes for cell imaging of protein sulfenylation and their application to monitor cisplatin induced protein oxidation},
url = {http://dx.doi.org/10.1016/j.snb.2017.03.171},
volume = {248},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Reactive oxygen species (ROS) are considered versatile second messengers mediating fundamental biological functions. A molecular pathway by which ROS determine functional diversity is the selective oxidation of cysteine residues to form sulfenic acid (SOH) products, known as sulfenylation or S-hydroxylation. This crucial post-translational modification is responsible for the alteration of protein stability, function and signalling. Despite considerable advances on the identification of sulfenic residues on individual proteins, improved methods are needed for direct visualization and accurate quantification of the extent of total protein sulfenylation. Herein we present the synthesis of two new cell-permeable fluorescent probes containing dimedone (a cyclic β-diketone with high specificity for sulfenic acids), and apply them to study oxidation processes in individual cells via microscopy. The low cytotoxicity, cell permeability and optical features of the probes allowed us to visualize and quantify the oxidation of cysteine residues in live cells during H2O2-mediated oxidative burst (i.e. exogenously administered H2O2). We present preliminary cellular imaging studies with these probes to analyse the oxidation process in cells treated with the anticancer drug cisplatin.
AU - Cilibrizzi,A
AU - Terenghi,M
AU - Fedorova,M
AU - Woscholski,R
AU - Klug,D
AU - Vilar,R
DO - 10.1016/j.snb.2017.03.171
EP - 446
PY - 2017///
SN - 0925-4005
SP - 437
TI - Small-molecule optical probes for cell imaging of protein sulfenylation and their application to monitor cisplatin induced protein oxidation
T2 - Sensors and Actuators B: Chemical
UR - http://dx.doi.org/10.1016/j.snb.2017.03.171
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000403772200056&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/50955
VL - 248
ER -