Imperial College London

Prof. Ramon Vilar

Faculty of Natural SciencesDepartment of Chemistry

Professor of Medicinal Inorganic Chemistry



+44 (0)20 7594 1967r.vilar Website




301HMolecular Sciences Research HubWhite City Campus






BibTex format

author = {Cilibrizzi, A and Fedorova, M and Collins, J and Leatherbarrow, R and Woscholski, R and Vilar, R},
doi = {10.1039/c7dt00076f},
pages = {6994--7004},
title = {A tri-functional vanadium(IV) complex to detect cysteine oxidation},
url = {},
volume = {46},
year = {2017}

RIS format (EndNote, RefMan)

AB - The development of effective molecular probes to detect and image the levels of oxidative stress in cells remains a challenge. Herein we report the design, synthesis and preliminary biological evaluation of a novel optical probe to monitor oxidation of thiol groups in cysteine-based phosphatases (CBPs). Following orthogonal protecting approaches we synthesised a new vanadyl complex designed to bind to CBPs. This complex is functionalised with a well-known dimedone derivative (to covalently trap sulfenic acids, SOHs) and a coumarin-based fluorophore for optical visualization. We show that this new probe efficiently binds to a range of phosphatases in vitro with nanomolar affinity. Moreover, preliminary flow cytometry and microscopy studies in live HCT116 cells show that this probe can successfully image cellular levels of sulfenic acids – one of the species resulting from protein oxidative damage.
AU - Cilibrizzi,A
AU - Fedorova,M
AU - Collins,J
AU - Leatherbarrow,R
AU - Woscholski,R
AU - Vilar,R
DO - 10.1039/c7dt00076f
EP - 7004
PY - 2017///
SN - 1477-9226
SP - 6994
TI - A tri-functional vanadium(IV) complex to detect cysteine oxidation
UR -
UR -
UR -
VL - 46
ER -