Imperial College London
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Prof. Ramon Vilar

Faculty of Natural Sciences, Department of Chemistry

Prof of Medicinal Inorganic Chemistry & Vice-Dean (Research)
 
 
 
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Contact

 

+44 (0)20 7594 1967r.vilar Website

 
 
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Location

 

301HMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Vilar:2021:10.1002/chem.202003875,
author = {Vilar, R and Lewis, BW and Bisballe, N and Santella, M and Summers, PA and Vannier, J-B and Kuimova, MK and Laursen, BW},
doi = {10.1002/chem.202003875},
journal = {Chemistry: A European Journal},
pages = {2523--2536},
title = {Assessing the key photophysical properties of triangulenium dyes for DNA binding by alteration of the fluorescent core},
url = {http://dx.doi.org/10.1002/chem.202003875},
volume = {27},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Four-stranded G-quadruplex (G4) DNA is a non-canonical DNA topology that has been proposed to form in cells and play key roles in how the genome is read and used by the cellular machinery. Previously, a fluorescent triangulenium probe (DAOTA-M2) was used to visualise G4s in cellulo, thanks to its distinct fluorescence lifetimes when bound to different DNA topologies. Herein, we expand the library of available triangulenium probes to explore how modifications to the fluorescent core of the molecule affect its photophysical characteristics, interaction with DNA and cellular localisation. The benzo-bridged and isopropyl-bridged diazatriangulenium dyes, BDATA-M2 and CDATA-M2 respectively, featuring ethyl-morpholino substituents, were synthesised and characterised. The interactions of these molecules with different DNA topologies were studied to determine their binding affinity, fluorescence enhancement and fluorescence lifetime response. Finally, the cellular uptake and localisation of these optical probes were investigated. Whilst structural modifications to the triangulenium core only slightly alter the binding affinity to DNA, BDATA-M2 and CDATA-M2 cannot distinguish between DNA topologies through their fluorescence lifetime. This work presents valuable new evidence into the critical role of PET quenching when using the fluorescence lifetime of triangulenium dyes to discriminate G4 DNA from duplex DNA, highlighting the importance of fine tuning redox and spectral properties when developing new triangulenium-based G4 probes.
AU  - Vilar,R
AU  - Lewis,BW
AU  - Bisballe,N
AU  - Santella,M
AU  - Summers,PA
AU  - Vannier,J-B
AU  - Kuimova,MK
AU  - Laursen,BW
DO  - 10.1002/chem.202003875
EP  - 2536
PY  - 2021///
SN  - 0947-6539
SP  - 2523
TI  - Assessing the key photophysical properties of triangulenium dyes for DNA binding by alteration of the fluorescent core
T2  - Chemistry: A European Journal
UR  - http://dx.doi.org/10.1002/chem.202003875
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33105523
UR  - https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202003875
UR  - http://hdl.handle.net/10044/1/85072
VL  - 27
ER  -
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