Imperial College London
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Prof. Ramon Vilar

Faculty of Natural Sciences, Department of Chemistry

Prof of Medicinal Inorganic Chemistry & Vice-Dean (Research)
 
 
 
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Contact

 

+44 (0)20 7594 1967r.vilar Website

 
 
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Location

 

301HMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Shivalingam:2015:10.1038/ncomms9178,
author = {Shivalingam, A and Izquierdo, MA and Le, Marois A and Vyniauskas, A and Suhling, K and Kuimova, MK and Vilar, R},
doi = {10.1038/ncomms9178},
journal = {Nature Communications},
pages = {1--10},
title = {The interactions between a small molecule and G-quadruplexes are visualised by fluorescence lifetime imaging microscopy},
url = {http://dx.doi.org/10.1038/ncomms9178},
volume = {6},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Guanine-rich oligonucleotides can fold into quadruple-stranded helical structures known as G-quadruplexes. Mounting experimental evidence has gathered suggesting that these non-canonical nucleic acid structures form in vivo and play essential biological roles. However, to date, there are no small-molecule optical probes to image G-quadruplexes in live cells. Herein, we report the design and development of a small fluorescent molecule, which can be used as an optical probe for G-quadruplexes. We demonstrate that the fluorescence lifetime of this new probe changes considerably upon interaction with different nucleic acid topologies. Specifically, longer fluorescence lifetimes are observed in vitro for G-quadruplexes than for double- and single-stranded nucleic acids. Cellular studies confirm that this molecule is cell permeable, has low cytotoxicity and localizes primarily in the cell nucleus. Furthermore, using fluorescence lifetime imaging microscopy, live-cell imaging suggests that the probe can be used to study the interaction of small molecules with G-quadruplexes in vivo.
AU  - Shivalingam,A
AU  - Izquierdo,MA
AU  - Le,Marois A
AU  - Vyniauskas,A
AU  - Suhling,K
AU  - Kuimova,MK
AU  - Vilar,R
DO  - 10.1038/ncomms9178
EP  - 10
PY  - 2015///
SN  - 2041-1723
SP  - 1
TI  - The interactions between a small molecule and G-quadruplexes are visualised by fluorescence lifetime imaging microscopy
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms9178
UR  - https://www.nature.com/articles/ncomms9178
UR  - http://hdl.handle.net/10044/1/26127
VL  - 6
ER  -
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