Imperial College London
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Dr Woscholski

Faculty of Natural Sciences, Department of Chemistry

Reader in Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 5305r.woscholski

 
 
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Location

 

301LMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cilibrizzi:2017:10.1039/c7dt00076f,
author = {Cilibrizzi, A and Fedorova, M and Collins, J and Leatherbarrow, R and Woscholski, R and Vilar, R},
doi = {10.1039/c7dt00076f},
journal = {DALTON TRANSACTIONS},
pages = {6994--7004},
title = {A tri-functional vanadium(IV) complex to detect cysteine oxidation},
url = {http://dx.doi.org/10.1039/c7dt00076f},
volume = {46},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The development of effective molecular probes to detect and image the levels of oxidative stress in cells remains a challenge. Herein we report the design, synthesis and preliminary biological evaluation of a novel optical probe to monitor oxidation of thiol groups in cysteine-based phosphatases (CBPs). Following orthogonal protecting approaches we synthesised a new vanadyl complex designed to bind to CBPs. This complex is functionalised with a well-known dimedone derivative (to covalently trap sulfenic acids, SOHs) and a coumarin-based fluorophore for optical visualization. We show that this new probe efficiently binds to a range of phosphatases in vitro with nanomolar affinity. Moreover, preliminary flow cytometry and microscopy studies in live HCT116 cells show that this probe can successfully image cellular levels of sulfenic acids – one of the species resulting from protein oxidative damage.
AU  - Cilibrizzi,A
AU  - Fedorova,M
AU  - Collins,J
AU  - Leatherbarrow,R
AU  - Woscholski,R
AU  - Vilar,R
DO  - 10.1039/c7dt00076f
EP  - 7004
PY  - 2017///
SN  - 1477-9226
SP  - 6994
TI  - A tri-functional vanadium(IV) complex to detect cysteine oxidation
T2  - DALTON TRANSACTIONS
UR  - http://dx.doi.org/10.1039/c7dt00076f
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000402550500020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/49710
VL  - 46
ER  -
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