Imperial College London
Alternate

ProfessorRylieGreen

Faculty of Engineering, Department of Bioengineering

Head of the Department of Bioengineering
 
 
 
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Contact

 

+44 (0)20 7594 0943rylie.green

 
 
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Location

 

3.05Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hassarati:2016:10.3389/fnins.2016.00432,
author = {Hassarati, RT and Foster, LJR and Green, RA},
doi = {10.3389/fnins.2016.00432},
journal = {Frontiers in Neuroscience},
title = {Influence of biphasic stimulation on olfactory ensheathing cells for neuroprosthetic devices},
url = {http://dx.doi.org/10.3389/fnins.2016.00432},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - � 2016 Hassarati, Foster and Green. The recent success of olfactory ensheathing cell (OEC) assisted regeneration of injured spinal cord has seen a rising interest in the use of these cells in tissue-engineered systems. Previously shown to support neural cell growth through glial scar tissue, OECs have the potential to assist neural network formation in living electrode systems to produce superior neuroprosthetic electrode surfaces. The following study sought to understand the influence of biphasic electrical stimulation (ES), inherent to bionic devices, on cell survival and function, with respect to conventional metallic and developmental conductive hydrogel (CH) coated electrodes. The CH utilized in this study was a biosynthetic hydrogel consisting of methacrylated poly(vinyl-alcohol) (PVA), heparin and gelatin through which poly(3,4-ethylenedioxythiophene) (PEDOT) was electropolymerised. OECs cultured on Pt and CH surfaces were subjected to biphasic ES. Image-based cytometry yielded little significant difference between the viability and cell cycle of OECs cultured on the stimulated and passive samples. The significantly lower voltages measured across the CH electrodes (147 � 3 mV) compared to the Pt (317 � 5 mV), had shown to influence a higher percentage of viable cells on CH (91-93%) compared to Pt (78-81%). To determine the functionality of these cells following electrical stimulation, OECs co-cultured with PC12 cells were found to support neural cell differentiation (an indirect measure of neurotrophic factor production) following ES.
AU  - Hassarati,RT
AU  - Foster,LJR
AU  - Green,RA
DO  - 10.3389/fnins.2016.00432
PY  - 2016///
SN  - 1662-453X
TI  - Influence of biphasic stimulation on olfactory ensheathing cells for neuroprosthetic devices
T2  - Frontiers in Neuroscience
UR  - http://dx.doi.org/10.3389/fnins.2016.00432
UR  - http://hdl.handle.net/10044/1/42305
VL  - 10
ER  -
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