Imperial College London

Professor Sir Steve Bloom FMedSci, FRS

Faculty of MedicineDepartment of Medicine

Departmental Academic REF2014 Lead
 
 
 
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Contact

 

+44 (0)20 3313 3242s.bloom Website

 
 
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Assistant

 

Ms Suneeta Nathan +44 (0)20 3313 3242

 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Publication Type
Year
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1985 results found

Abbara A, Clarke S, Islam R, Prague JK, Comninos AN, Narayanaswamy S, Papadopoulou D, Roberts R, Izzi-Engbeaya C, Ratnasabapathy R, Nesbitt A, Vimalesvaran S, Salim R, Lavery SA, Bloom SR, Huson L, Trew GH, Dhillo WSet al., 2017, A second dose of kisspeptin-54 improves oocyte maturation in women at high risk of ovarian hyperstimulation syndrome: a Phase 2 randomized controlled trial, HUMAN REPRODUCTION, Vol: 32, Pages: 1915-1924, ISSN: 0268-1161

JOURNAL ARTICLE

Alamshah A, Spreckley E, Norton M, Kinsey-Jones JS, Amin A, Ramgulam A, Cao Y, Johnson R, Saleh K, Akalestou E, Malik Z, Gonzalez-Abuin N, Jomard A, Amarsi R, Moolla A, Sargent PR, Gray GW, Bloom SR, Murphy KGet al., 2017, l-phenylalanine modulates gut hormone release and glucose tolerance, and suppresses food intake through the calcium-sensing receptor in rodents., Int J Obes (Lond)

OBJECTIVE: High-protein diets (HPDs) are associated with greater satiety and weight loss than diets rich in other macronutrients. The exact mechanisms by which HPDs exert their effects are unclear. However, evidence suggests that the sensing of amino acids produced as a result of protein digestion may have a role in appetite regulation and satiety. We investigated the effects of l-phenylalanine (L-Phe) on food intake and glucose homeostasis in rodents. METHODS: We investigated the effects of the aromatic amino-acid and calcium-sensing receptor (CaSR) agonist l-phenylalanine (L-Phe) on food intake and the release of the gastrointestinal (GI) hormones peptide YY (PYY), glucagon-like peptide-1 (GLP-1) and ghrelin in rodents, and the role of the CaSR in mediating these effects in vitro and in vivo. We also examined the effect of oral l-Phe administration on glucose tolerance in rats. RESULTS: Oral administration of l-Phe acutely reduced food intake in rats and mice, and chronically reduced food intake and body weight in diet-induced obese mice. Ileal l-Phe also reduced food intake in rats. l-Phe stimulated GLP-1 and PYY release, and reduced plasma ghrelin, and also stimulated insulin release and improved glucose tolerance in rats. Pharmacological blockade of the CaSR attenuated the anorectic effect of intra-ileal l-Phe in rats, and l-Phe-induced GLP-1 release from STC-1 and primary L cells was attenuated by CaSR blockade. CONCLUSIONS: l-Phe reduced food intake, stimulated GLP-1 and PYY release, and reduced plasma ghrelin in rodents. Our data provide evidence that the anorectic effects of l-Phe are mediated via the CaSR, and suggest that l-Phe and the CaSR system in the GI tract may have therapeutic utility in the treatment of obesity and diabetes. Further work is required to determine the physiological role of the CaSR in protein sensing in the gut, and the role of this system in humans.International Journal of Obesity advance online publication, 8 August 2017; doi:10.1

JOURNAL ARTICLE

Brooks L, Viardot A, Tsakmaki A, Stolarczyk E, Howard JK, Cani PD, Everard A, Sleeth ML, Psichas A, Anastasovskaj J, Bell JD, Bell-Anderson K, Mackay CR, Ghatei MA, Bloom SR, Frost G, Bewick GAet al., 2017, Fermentable carbohydrate stimulates FFAR2-dependent colonic PYY cell expansion to increase satiety, MOLECULAR METABOLISM, Vol: 6, Pages: 48-60, ISSN: 2212-8778

JOURNAL ARTICLE

Buenaventura T, Kanda N, Jones B, Correa IR, Bloom SR, Rutter GA, Tomas Aet al., 2017, Characterisation of glucagon-like peptide-1 (GLP-1) receptor trafficking and its significance for pancreatic beta cell function, DIABETIC MEDICINE, Vol: 34, Pages: 53-53, ISSN: 0742-3071

JOURNAL ARTICLE

Cegla J, Jones BJ, Gardiner JV, Hodson DJ, Marjot T, McGlone ER, Tan TM, Bloom SRet al., 2017, RAMP2 Influences Glucagon Receptor Pharmacology via Trafficking and Signaling, 52nd Annual Meeting of the European-Association-for-the-Study-of-Diabetes (EASD), Publisher: OXFORD UNIV PRESS INC, Pages: 2680-2693, ISSN: 0013-7227

CONFERENCE PAPER

Cegla J, Jones BJ, Howard J, Kay R, Creaser CS, Bloom SR, Tan TMet al., 2017, The preanalytical stability of glucagon as measured by liquid chromatography tandem mass spectrometry and two commercially available immunoassays, ANNALS OF CLINICAL BIOCHEMISTRY, Vol: 54, Pages: 293-296, ISSN: 0004-5632

JOURNAL ARTICLE

Christakis I, Scott R, Minnion J, Cuenco J, Tan T, Palazzo F, Bloom Set al., 2017, Measuring the Pharmacokinetic Properties of Drugs with a Novel Surgical Rat Model, JOURNAL OF INVESTIGATIVE SURGERY, Vol: 30, Pages: 162-169, ISSN: 0894-1939

JOURNAL ARTICLE

Comninos AN, Wall MB, Demetriou L, Shah AJ, Clarke SA, Narayanaswamy S, Nesbitt A, Izzi-Engbeaya C, Prague JK, Abbara A, Ratnasabapathy R, Salem V, Nijher GM, Jayasena CN, Tanner M, Bassett P, Mehta A, Rabiner EA, Honigsperger C, Silva MR, Brandtzaeg OK, Lundanes E, Wilson SR, Brown RC, Thomas SA, Bloom SR, Dhillo WSet al., 2017, Kisspeptin modulates sexual and emotional brain processing in humans, JOURNAL OF CLINICAL INVESTIGATION, Vol: 127, Pages: 709-719, ISSN: 0021-9738

JOURNAL ARTICLE

Cork SC, Eftekhar A, Mirza KB, Gardiner JV, Bloom SR, Toumazou Cet al., 2017, Extracellular pH monitoring for use in closed-loop vagus nerve stimulation., J Neural Eng

OBJECTIVE: Vagal nerve stimulation (VNS) has shown potential benefits for obesity treatment; however current devices lack physiological feedback, which limit their efficacy. Changes in extracellular pH (pH<sub>e</sub>) have shown to be correlated with neural activity, but have traditionally been measured with glass microelectrodes, which limit their in vivo applicability. APPROACH: Iridium oxide has previously been shown to be sensitive to fluctuations in pH and is biocompatible. Iridium oxide microelectrodes were inserted into the subdiaphragmatic vagus nerve of anaesthetised rats. Introduction of the gut hormone cholecystokinin (CCK) or distension of the stomach was used to elicit vagal nerve activity. MAIN RESULTS: iridium oxide microelectrodes have sufficient pH sensitivity to readily detect changes in pH<sub>e</sub> associated with both CCK and gastric distension. What's more, custom made Matlab script was able to use these changes in pH<sub>e</sub> to automatically trigger an implanted VNS device. SIGNIFICANCE: This is the first study to show pH<sub>e</sub> changes in peripheral nerves in vivo. Furthermore, the demonstration that iridium oxide microelectrodes are sufficiently pH sensitive as to measure changes in pH<sub>e</sub> associated with physiological stimuli means they have the potential to be integrated into closed-loop neurostimulating devices.

JOURNAL ARTICLE

Cuenco J, Minnion J, Tan T, Scott R, Germain N, Ling Y, Chen R, Ghatei M, Bloom Set al., 2017, Degradation Paradigm of the Gut Hormone, Pancreatic Polypeptide, by Hepatic and Renal Peptidases, ENDOCRINOLOGY, Vol: 158, Pages: 1755-1765, ISSN: 0013-7227

JOURNAL ARTICLE

Hameed S, Patterson M, Dhillo WS, Rahman SA, Ma Y, Holton C, Gogakos A, Yeo GSH, Lam BYH, Polex-Wolf J, Fenske W, Bell J, Anastasovska J, Samarut J, Bloom SR, Bassett JHD, Williams GR, Gardiner JVet al., 2017, Thyroid Hormone Receptor Beta in the Ventromedial Hypothalamus Is Essential for the Physiological Regulation of Food Intake and Body Weight, CELL REPORTS, Vol: 19, Pages: 2202-2209, ISSN: 2211-1247

JOURNAL ARTICLE

Howard JW, Kay RG, Jones B, Cegla J, Tan T, Bloom S, Creaser CSet al., 2017, Development of a UHPLC-MS/MS (SRM) method for the quantitation of endogenous glucagon and dosed GLP-1 from human plasma, BIOANALYSIS, Vol: 9, Pages: 733-751, ISSN: 1757-6180

JOURNAL ARTICLE

Jones BJ, Scopelliti R, Tomas A, Bloom SR, Hodson DJ, Broichhagen Jet al., 2017, Potent Prearranged Positive Allosteric Modulators of the Glucagon-like Peptide-1 Receptor, CHEMISTRYOPEN, Vol: 6, Pages: 501-505, ISSN: 2191-1363

JOURNAL ARTICLE

Keetarut K, Zacharopoulou-Otapasidou S, Bloom S, Majumdar A, Patel PSet al., 2017, An evaluation of the feasibility and validity of a patient-administered malnutrition universal screening tool ('MUST') compared to healthcare professional screening in an inflammatory bowel disease (IBD) outpatient clinic., J Hum Nutr Diet

BACKGROUND: Malnutrition is common in inflammatory bowel disease (IBD) and is associated with poor health outcomes. Despite this, screening for malnutrition in the outpatient-setting is not routine and research in the area is limited. The present study aimed to evaluate whether agreement between malnutrition screening completed by patients and healthcare professionals (HCPs) could be achieved by comparing patient self-administered 'MUST' ('MUST'-P) with HCP administered 'MUST' ('MUST'-HCP) in a single tertiary IBD outpatient clinic. METHODS: We conducted a feasibility and validity study on adult outpatients with IBD. We collected anthropometric, nutritional and clinical data from patients. All patients completed 'MUST'-P using a self-administered questionnaire, followed by 'MUST'-HCP. 'MUST'-P was timed and feedback on ease-of-use was obtained. The risk of malnutrition was classified as low (score = 0), medium (score = 1) and high (score ≥ 2) and agreement was tested using kappa statistics (κ). RESULTS: Eighty patients were recruited (Crohn's disease: n = 49, ulcerative colitis: n = 29, unclassified: n = 2), with a mean (SD) age of 39.9 (15.1) years (51.2% were males). Seventy-one (92%) of patients found 'MUST'-P either easy or very easy. The mean (SD) time to complete 'MUST'-P was 3.1  (1.8) min (range 1-10 min). Sixty-eight (85%) of patients were at low risk of malnutrition when screened by the HCP. There was moderate agreement (κ = 0.486, P < 0.001) between 'MUST'-P and 'MUST'-HCP, with 100% agreement in scoring for medium- and high-risk categories. CONCLUSIONS: The results of the present study suggests that self-screening using 'MUST' could be effectively used in an IBD outpatient clinic to identify those at medium and high risk of malnutrition. The patient friendly version of 'MUST' ('MUST'-P) was considered quick and easy to use by patient

JOURNAL ARTICLE

Law JM, Morris DE, Engbeaya CI, Salem V, Coello C, Robinson L, Jayasinghe M, Scott R, Gunn R, Rabiner E, Tan T, Dhillo W, Bloom S, Budge H, Symonds Met al., 2017, Thermal imaging is a non-invasive alternative to PET-CT for measurement of brown adipose tissue activity in humans., J Nucl Med

Background: Obesity and its metabolic consequences are a major cause of morbidity and mortality. Brown adipose tissue (BAT) utilises glucose and free fatty acids to produce heat, thereby increasing energy expenditure. Effective evaluation of human BAT stimulators is constrained by current standard BAT assessment methods as positron emission tomography-computed tomography (PET-CT) requires exposure to high doses of ionising radiation. Infrared thermography (IRT) is a potential non-invasive, safe alternative, although direct corroboration with PET-CT has not previously been established. Methods: IRT and (18)F-FDG PET-CT data from 8 healthy male participants subjected to water jacket cooling were directly compared. Thermal images (TIs) were geometrically transformed to overlay PET-CT-derived maximum intensity projection (MIP) images from each subject and the areas of greatest intensity of temperature and glucose-uptake within the supraclavicular regions compared. Relationships between supraclavicular temperatures from IRT (TSCR) and the maximum rate of glucose uptake (MR(gluc)) from PET-CT were determined. Results: Glucose uptake on MR(gluc)MIP was positively correlated with change in TSCR relative to a reference region (r2 = 0.721; P = 0.008). Spatial overlap between areas of maximal MR(gluc)MIP and maximal TSCR was 29.5±5.1%. Prolonged cooling to 60 minutes was associated with further TSCR rise compared with cooling to 10 minutes. Conclusion: The supraclavicular hotspot identified on IRT closely corresponds to the area of maximal uptake on PET-CT-derived MR(gluc)MIP images. Greater increases in relative TSCR were associated with raised glucose uptake. IRT should now be considered a suitable method for measuring BAT activation, especially in populations where PET-CT is not feasible, practical or repeatable. <br /><div align="center"><font size="-2"><a rel="license" href="http://creativecommons.org/licenses

JOURNAL ARTICLE

Prague JK, Roberts RE, Comninos AN, Clarke S, Jayasena CN, Nash Z, Doyle C, Papadopoulou DA, Bloom SR, Mohideen P, Panay N, Hunter MS, Veldhuis JD, Webber LC, Huson L, Dhillo WSet al., 2017, Neurokinin 3 receptor antagonism as a novel treatment for menopausal hot flushes: a phase 2, randomised, double-blind, placebo-controlled trial, LANCET, Vol: 389, Pages: 1809-1820, ISSN: 0140-6736

JOURNAL ARTICLE

Tan T, Behary P, Tharakan G, Minnion J, Al-Najim W, Albrechtsen NJW, Holst JJ, Bloom SRet al., 2017, The Effect of a Subcutaneous Infusion of GLP-1, OXM, and PYY on Energy Intake and Expenditure in Obese Volunteers, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, Vol: 102, Pages: 2364-2372, ISSN: 0021-972X

JOURNAL ARTICLE

Tharakan G, Behary P, Wewer Albrechtsen NJ, Chahal H, Kenkre J, Miras AD, Ahmed AR, Holst JJ, Bloom SR, Tan TMMet al., 2017, Roles of increased glycemic variability, GLP-1 and glucagon in hypoglycaemia after Roux-en-Y gastric bypass., Eur J Endocrinol

Objective Roux-en-Y Gastric Bypass (RYGB) surgery is currently the most effective treatment for diabetes and obesity. An increasingly recognized complication of RYGB surgery is postprandial hypoglycemia (PPH). The pathophysiology of PPH remains unclear with multiple mechanisms suggested including nesidioblastosis, altered insulin clearance and increased glucagon-like-1 peptide (GLP-1) secretion. Whilst many PPH patients respond to dietary modification, some have severely disabling symptoms. Multiple treatments have been trialled ranging from acarbose, to both GLP-1 agonists and antagonists, even to reversal of RYGB. A greater understanding of the pathophysiology of PPH could guide the development of new therapeutic strategies. Methods We studied a cohort of PPH patients at the Imperial Weight Center. We performed continuous glucose monitoring to characterize their altered glycemic variability. We also performed a mixed meal test (MMT) and measured gut hormone concentrations. Results We found increased glycemic variability in our cohort of PPH patients, specifically a higher Mean Amplitude Glucose Excursion (MAGE) score of 4.9. We also demonstrated significantly greater and earlier increases in insulin and GLP-1 concentration in patients who had hypoglycemia in response to an MMT (MMT Hypo) relative to those that did not (MMT Non-Hypo). There was a significantly increased glucagon secretion in the MMT Hypo group versus the Non-hypo group. No significant differences in oxyntomodulin, GIP or peptide YY secretion were seen between these two groups. Conclusion An early peak in GLP-1 and glucagon, due to post-operative L-cell hypertrophy and aberrant processing of proglucagon, may trigger an exaggerated insulinotropic response to eating in patients with PPH.

JOURNAL ARTICLE

Akalestou E, Christakis I, Solomou AM, Minnion JS, Rutter GA, Bloom SRet al., 2016, Proglucagon-Derived Peptides Do Not Significantly Affect Acute Exocrine Pancreas in Rats, PANCREAS, Vol: 45, Pages: 967-973, ISSN: 0885-3177

JOURNAL ARTICLE

Alamshah A, McGavigan AK, Spreckley E, Kinsey-Jones JS, Amin A, Tough IR, O'Hara HC, Moolla A, Banks K, France R, Hyberg G, Norton M, Cheong W, Lehmann A, Bloom SR, Cox HM, Murphy KGet al., 2016, L-arginine promotes gut hormone release and reduces food intake in rodents, DIABETES OBESITY & METABOLISM, Vol: 18, Pages: 508-518, ISSN: 1462-8902

JOURNAL ARTICLE

Broichhagen J, Johnston NR, von Ohlen Y, Meyer-Berg H, Jones BJ, Bloom SR, Rutter GA, Trauner D, Hodson DJet al., 2016, Allosteric Optical Control of a Class B G-Protein-Coupled Receptor, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 55, Pages: 5865-5868, ISSN: 1433-7851

JOURNAL ARTICLE

Broichhagen J, von Ohlen Y, Johnston NR, Meyer-Berg H, Jones BJ, Bloom SR, Rutter GA, Trauner D, Hodson DJet al., 2016, Optical control of the glucagon-like peptide-1 receptor allosteric site, DIABETIC MEDICINE, Vol: 33, Pages: 60-60, ISSN: 0742-3071

JOURNAL ARTICLE

Choudhury SM, Tan TM, Bloom SR, 2016, Gastrointestinal hormones and their role in obesity, CURRENT OPINION IN ENDOCRINOLOGY DIABETES AND OBESITY, Vol: 23, Pages: 18-22, ISSN: 1752-296X

JOURNAL ARTICLE

Comninos AN, Anastasovska J, Sahuri-Arisoylu M, Li X, Li S, Hu M, Jayasena CN, Ghatei MA, Bloom SR, Matthews PM, O'Byrne KT, Bell JD, Dhillo WSet al., 2016, Kisspeptin signaling in the amygdala modulates reproductive hormone secretion, BRAIN STRUCTURE & FUNCTION, Vol: 221, Pages: 2035-2047, ISSN: 1863-2653

JOURNAL ARTICLE

De Backer I, Hussain SS, Bloom SR, Gardiner JVet al., 2016, Insights into the role of neuronal glucokinase, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, Vol: 311, Pages: E42-E55, ISSN: 0193-1849

JOURNAL ARTICLE

Jayasena CN, Abbara A, Comninos AN, Narayanaswamy S, Maffe JG, Izzi-Engbeaya C, Oldham J, Lee TTM, Sarang Z, Malik Z, Dhanjal MK, Williamson C, Regan L, Bloom SR, Dhillo WSet al., 2016, Novel circulating placental markers prokineticin-1, soluble fms-like tyrosine kinase-1, soluble endoglin and placental growth factor and association with late miscarriage, HUMAN REPRODUCTION, Vol: 31, Pages: 2681-2688, ISSN: 0268-1161

JOURNAL ARTICLE

Jones B, Buenaventura T, Owen B, Correa IR, Johnson P, Bosco D, Bloom SR, Rutter GA, Tomas Aet al., 2016, Biased agonism alters GLP-1 receptor trafficking and glucose homeostasis, 52nd Annual Meeting of the European-Association-for-the-Study-of-Diabetes (EASD), Publisher: SPRINGER, Pages: S72-S73, ISSN: 0012-186X

CONFERENCE PAPER

Narayanaswamy S, Jayasena CN, Ng N, Ratnasabapathy R, Prague JK, Papadopoulou D, Abbara A, Comninos AN, Bassett P, Bloom SR, Veldhuis JD, Dhillo WSet al., 2016, Subcutaneous infusion of kisspeptin-54 stimulates gonadotrophin release in women and the response correlates with basal oestradiol levels, CLINICAL ENDOCRINOLOGY, Vol: 84, Pages: 939-945, ISSN: 0300-0664

JOURNAL ARTICLE

Narayanaswamy S, Prague JK, Jayasena CN, Papadopoulou DA, Mizamtsidi M, Shah AJ, Bassett P, Comninos AN, Abbara A, Bloom SR, Veldhuis JD, Dhillo WSet al., 2016, Investigating the KNDy Hypothesis in Humans by Coadministration of Kisspeptin, Neurokinin B, and Naltrexone in Men, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, Vol: 101, Pages: 3429-3436, ISSN: 0021-972X

JOURNAL ARTICLE

Polyviou T, MacDougall K, Chambers ES, Viardot A, Psichas A, Jawaid S, Harris HC, Edwards CA, Simpson L, Murphy KG, Zac-Varghese SEK, Blundell JE, Dhillo WS, Bloom SR, Frost GS, Preston T, Tedford MC, Morrison DJet al., 2016, Randomised clinical study: inulin short-chain fatty acid esters for targeted delivery of short-chain fatty acids to the human colon, ALIMENTARY PHARMACOLOGY & THERAPEUTICS, Vol: 44, Pages: 662-672, ISSN: 0269-2813

JOURNAL ARTICLE

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