Imperial College London
Alternate

ProfessorStephenBrickley

Faculty of Natural Sciences, Department of Life Sciences

Professor of Systems Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 7699s.brickley

 
 
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Location

 

402ASir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Song:2018:10.1016/j.celrep.2018.06.076,
author = {Song, JH and Lucaci, D and Calangiu, I and Brown, MTC and Park, JS and Kim, J and Brickley, SG and Chadderton, PT},
doi = {10.1016/j.celrep.2018.06.076},
journal = {Cell Reports},
pages = {1071--1080},
title = {Combining mGRASP and optogenetics enables high-resolution functional mapping of descending cortical projections},
url = {http://dx.doi.org/10.1016/j.celrep.2018.06.076},
volume = {24},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We have applied optogenetics and mGRASP, a light microscopy technique that labels synaptic contacts, to map the number and strength of defined corticocollicular (CC) connections. Using mGRASP, we show that CC projections form small, medium, and large synapses, and both the number and the distribution of synapse size vary among the IC regions. Using optogenetics, we show that low-frequency stimulation of CC axons expressing channelrhodopsin produces prolonged elevations of the CC miniature EPSC (mEPSC) rate. Functional analysis of CC mEPSCs reveals small-, medium-, and large-amplitude events that mirror the synaptic distributions observed with mGRASP. Our results reveal that descending ipsilateral projections dominate CC feedback via an increased number of large synaptic contacts, especially onto the soma of IC neurons. This study highlights the feasibility of combining microscopy (i.e., mGRASP) and optogenetics to reveal synaptic weighting of defined projections at the level of single neurons, enabling functional connectomic mapping in diverse neural circuits.
AU  - Song,JH
AU  - Lucaci,D
AU  - Calangiu,I
AU  - Brown,MTC
AU  - Park,JS
AU  - Kim,J
AU  - Brickley,SG
AU  - Chadderton,PT
DO  - 10.1016/j.celrep.2018.06.076
EP  - 1080
PY  - 2018///
SN  - 2211-1247
SP  - 1071
TI  - Combining mGRASP and optogenetics enables high-resolution functional mapping of descending cortical projections
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2018.06.076
UR  - http://hdl.handle.net/10044/1/61582
VL  - 24
ER  -
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