Imperial College London

ProfessorStephenCurry

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Structural Biology
 
 
 
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Contact

 

+44 (0)20 7594 7632s.curry Website

 
 
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Assistant

 

Mrs Jacalyn Murphy +44 (0)20 7594 1919

 
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Location

 

404ASir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

124 results found

Curry S, 2012, By the people, for the people, New Scientist, Vol: 214, Pages: 26-27, ISSN: 0262-4079

Journal article

Martino L, Pennell S, Kelly G, Bui TTT, Kotik-Kogan O, Smerdon SJ, Drake AF, Curry S, Conte MRet al., 2012, Analysis of the interaction with the hepatitis C virus mRNA reveals an alternative mode of RNA recognition by the human La protein, NUCLEIC ACIDS RESEARCH, Vol: 40, Pages: 1381-1394, ISSN: 0305-1048

Journal article

Joshi A, Coelho MB, Kotik-Kogan O, Simpson PJ, Matthews SJ, Smith CW, Curry Set al., 2011, Crystallographic analysis of polypyrimidine tract-binding protein-Raver1 interactions involved in regulation of alternative splicing., Structure, Vol: 19, Pages: 1816-1825

The polypyrimidine tract-binding protein (PTB) is an important regulator of alternative splicing. PTB-regulated splicing of α-tropomyosin is enhanced by Raver1, a protein with four PTB-Raver1 interacting motifs (PRIs) that bind to the helical face of the second RNA recognition motif (RRM2) in PTB. We present the crystal structures of RRM2 in complex with PRI3 and PRI4 from Raver1, which--along with structure-based mutagenesis--reveal the molecular basis of their differential binding. High-affinity binding by Raver1 PRI3 involves shape-matched apolar contacts complemented by specific hydrogen bonds, a new variant of an established mode of peptide-RRM interaction. Our results refine the sequence of the PRI motif and place important structural constraints on functional models of PTB-Raver1 interactions. Our analysis indicates that the observed Raver1-PTB interaction is a general mode of binding that applies to Raver1 complexes with PTB paralogues such as nPTB and to complexes of Raver2 with PTB.

Journal article

Jamal SM, Ferrari G, Ahmed S, Normann P, Curry S, Belsham GJet al., 2011, Evolutionary analysis of serotype A foot-and-mouth disease viruses circulating in Pakistan and Afghanistan during 2002-2009, JOURNAL OF GENERAL VIROLOGY, Vol: 92, Pages: 2849-2864, ISSN: 0022-1317

Journal article

Kafasla P, Lin H, Curry S, Jackson RJet al., 2011, Activation of picornaviral IRESs by PTB shows differential dependence on each PTB RNA-binding domain, RNA-A PUBLICATION OF THE RNA SOCIETY, Vol: 17, Pages: 1120-1131, ISSN: 1355-8382

Journal article

Nishi K, Ono T, Nakamura T, Fukunaga N, Izumi M, Watanabe H, Suenaga A, Maruyama T, Yamagata Y, Curry S, Otagiri Met al., 2011, Structural Insights into Differences in Drug-binding Selectivity between Two Forms of Human α1-Acid Glycoprotein Genetic Variants, the A and F1*S Forms, Journal of Biological Chemistry, Vol: 286, Pages: 14427-14434, ISSN: 1083-351X

Human α(1)-acid glycoprotein (hAGP) in serum functions as a carrier of basic drugs. In most individuals, hAGP exists as a mixture of two genetic variants, the F1*S and A variants, which bind drugs with different selectivities. We prepared a mutant of the A variant, C149R, and showed that its drug-binding properties were indistinguishable from those of the wild type. In this study, we determined the crystal structures of this mutant hAGP alone and complexed with disopyramide (DSP), amitriptyline (AMT), and the nonspecific drug chlorpromazine (CPZ). The crystal structures revealed that the drug-binding pocket on the A variant is located within an eight-stranded β-barrel, similar to that found in the F1*S variant and other lipocalin family proteins. However, the binding region of the A variant is narrower than that of the F1*S variant. In the crystal structures of complexes with DSP and AMT, the two aromatic rings of each drug interact with Phe-49 and Phe-112 at the bottom of the binding pocket. Although the structure of CPZ is similar to those of DSP and AMT, its fused aromatic ring system, which is extended in length by the addition of a chlorine atom, appears to dictate an alternative mode of binding, which explains its nonselective binding to the F1*S and A variant hAGPs. Modeling experiments based on the co-crystal structures suggest that, in complexes of DSP, AMT, or CPZ with the F1*S variant, Phe-114 sterically hinders interactions with DSP and AMT, but not CPZ.

Journal article

Ryan AJ, Ghuman J, Zunszain PA, Chung C-W, Curry Set al., 2010, Structural basis of binding of fluorescent, site-specific dansylated amino acids to human serum albumin, Journal of Structural Biology, Vol: 174, Pages: 84-91, ISSN: 1095-8657

Journal article

Sweeney TR, Cisnetto V, Bose D, Bailey M, Wilson JR, Zhang X, Belsham GJ, Curry Set al., 2010, Foot-and-Mouth Disease Virus 2C Is a Hexameric AAA plus Protein with a Coordinated ATP Hydrolysis Mechanism, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 24347-24359

Journal article

Klopfleisch C, Minh LQ, Giesow K, Curry S, Keil GMet al., 2010, Effect of foot-and-mouth disease virus capsid precursor protein and 3C protease expression on bovine herpesvirus 1 replication, ARCHIVES OF VIROLOGY, Vol: 155, Pages: 723-731, ISSN: 0304-8608

Journal article

Curry S, 2010, More light and colour. Science and the new media, Biochemist, Vol: 32, Pages: 18-21, ISSN: 0954-982X

Journal article

Zunszain PA, Knox SR, Sweeney TR, Yang J, Roque-Rosell N, Belsham GJ, Leatherbarrow RJ, Curry Set al., 2010, Insights into Cleavage Specificity from the Crystal Structure of Foot-and-Mouth Disease Virus 3C Protease Complexed with a Peptide Substrate, JOURNAL OF MOLECULAR BIOLOGY, Vol: 395, Pages: 375-389, ISSN: 0022-2836

Journal article

Curry S, Kotik-Kogan O, Conte MR, Brick Pet al., 2009, Getting to the end of RNA: Structural analysis of protein recognition of 5 ' and 3 ' termini, BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, Vol: 1789, Pages: 653-666, ISSN: 1874-9399

Journal article

Liu Y, Garnett JA, Leon E, Allman SA, Friedrich N, Saouros S, Curry S, Soldati-Favre D, Davis BG, Feizi T, Matthews Set al., 2009, Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii, Protein Sci., Vol: 18, Pages: 1935-1947

The intracellular protozoan Toxoplasma gondii is among the most widespread parasites. The broad host cell range of the parasite can be explained by carbohydrate microarray screening analyses that have demonstrated the ability of the T. gondii adhesive protein, TgMIC1, to bind to a wide spectrum of sialyl oligosaccharide ligands. Here, we investigate by further microarray analyses in a dose-response format the differential binding of TgMIC1 to 2-3- and 2-6-linked sialyl carbohydrates. Interestingly, two novel synthetic fluorinated analogs of 3'SiaLacNAc(1-4) and 3'SiaLacNAc(1-3) were identified as highly potent ligands. To understand the structural basis of the carbohydrate binding specificity of TgMIC1, we have determined the crystal structures of TgMIC1 micronemal adhesive repeat (MAR)-region (TgMIC1-MARR) in complex with five sialyl-N-acetyllactosamine analogs. These crystal structures have revealed a specific, water-mediated hydrogen bond network that accounts for the preferential binding of TgMIC1-MARR to arrayed 2-3-linked sialyl oligosaccharides and the high potency of the fluorinated analogs. Furthermore, we provide strong evidence for the first observation of a C--F...H--O hydrogen bond within a lectin-carbohydrate complex. Finally, detailed comparison with other oligosaccharide-protein complexes in the Protein Data Bank (PDB) reveals a new family of sialic-acid binding sites from lectins in parasites, bacteria, and viruses

Journal article

Nakagawa A, Komatsu T, Curry S, Tsuchida Eet al., 2009, O-2 Binding Properties of Human Serum Albumin Quadruple Mutant Complexed Iron Protoporphyrin IX with Axial His-186 Coordination, CHEMISTRY LETTERS, Vol: 38, Pages: 776-777, ISSN: 0366-7022

Journal article

Kafasla P, Morgner N, Poeyry TAA, Curry S, Robinson CV, Jackson RJet al., 2009, Polypyrimidine Tract Binding Protein Stabilizes the Encephalomyocarditis Virus IRES Structure via Binding Multiple Sites in a Unique Orientation, MOLECULAR CELL, Vol: 34, Pages: 556-568, ISSN: 1097-2765

Journal article

Curry S, 2009, Widen the channels of communication with society, NATURE, Vol: 458, Pages: 702-703, ISSN: 0028-0836

Journal article

Curry S, 2009, Lessons from the Crystallographic Analysis of Small Molecule Binding to Human Serum Albumin, DRUG METABOLISM AND PHARMACOKINETICS, Vol: 24, Pages: 342-357, ISSN: 1347-4367

Journal article

Komatsu T, Nakagawa A, Curry S, Tsuchida E, Murata K, Nakamura N, Ohno Het al., 2009, The role of an amino acid triad at the entrance of the heme pocket in human serum albumin for O-2 and CO binding to iron protoporphyrin IX, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol: 7, Pages: 3836-3841, ISSN: 1477-0520

Journal article

Sanfelice D, Kelly G, Curry S, Conte MRet al., 2008, NMR assignment of the N-terminal region of human La free and in complex with RNA, BIOMOLECULAR NMR ASSIGNMENTS, Vol: 2, Pages: 107-109, ISSN: 1874-2718

Journal article

Zunszain PA, Ghuman J, McDonagh AF, Curry Set al., 2008, Crystallographic analysis of human serum albumin complexed with 4Z,15E-bilirubin-IX alpha, JOURNAL OF MOLECULAR BIOLOGY, Vol: 381, Pages: 394-406, ISSN: 0022-2836

Journal article

Kotik-Kogan O, Valentine ER, Sanfelice D, Conte MR, Curry Set al., 2008, Structural analysis reveals conformational plasticity in the recognition of RNA 3 ' ends by the human La protein, STRUCTURE, Vol: 16, Pages: 852-862, ISSN: 0969-2126

Journal article

Jaulent AM, Fahy AS, Knox SR, Birtley JR, Roque-Rosell N, Curry S, Leatherbarrow RJet al., 2007, A continuous assay for foot-and-mouth disease virus 3C protease activity, ANALYTICAL BIOCHEMISTRY, Vol: 368, Pages: 130-137, ISSN: 0003-2697

Journal article

Komatsu T, Nakagawa A, Zunszain PA, Curry S, Tsuchida Eet al., 2007, Genetic engineering of the heme pocket in human serum albumin: Modulation of O-2 binding of iron protoporphyrin IX by variation of distal amino acids, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 129, Pages: 11286-11295, ISSN: 0002-7863

Journal article

Monie TP, Perrin AJ, Birtley JR, Sweeney TR, Karakasiliotis I, Chaudhry Y, Roberts LO, Matthews S, Goodfellow IG, Curry Set al., 2007, Structural insights into the transcriptional and translational roles of Ebp1, EMBO JOURNAL, Vol: 26, Pages: 3936-3944, ISSN: 0261-4189

Journal article

Roque-Rosell N, Curry S, Leatherbarrow RJ, 2007, Studies toward a new foot-and-mouth disease antiviral agent, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol: 233, Pages: 515-515, ISSN: 0065-7727

Journal article

Curry S, Roque-Rosell N, Zunszain PA, Leatherbarrow RJet al., 2007, Foot-and-mouth disease virus 3C protease: Recent structural and functional insights into an antiviral target, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol: 39, Pages: 1-6, ISSN: 1357-2725

Journal article

Sweeney TR, Roque-Rosell N, Birtley JR, Leatherbarrow RJ, Curry Set al., 2007, Structural and mutagenic analysis of foot-and-mouth disease virus 3C protease reveals the role of the beta-ribbon in proteolysis, JOURNAL OF VIROLOGY, Vol: 81, Pages: 115-124, ISSN: 0022-538X

Journal article

Ryan A, Ghuman J, Zunszain PA, Curry Set al., 2007, A Study of the Binding of Dansylated Amino Acids to Human Serum Albumin, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S19-S19, ISSN: 2053-2733

Conference paper

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