Imperial College London

ProfessorStephenCurry

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Structural Biology
 
 
 
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Contact

 

+44 (0)20 7594 7632s.curry Website

 
 
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Assistant

 

Mrs Jacalyn Murphy +44 (0)20 7594 1919

 
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Location

 

404ASir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Joshi:2011:10.1016/j.str.2011.09.020,
author = {Joshi, A and Coelho, MB and Kotik-Kogan, O and Simpson, PJ and Matthews, SJ and Smith, CW and Curry, S},
doi = {10.1016/j.str.2011.09.020},
journal = {Structure},
pages = {1816--1825},
title = {Crystallographic analysis of polypyrimidine tract-binding protein-Raver1 interactions involved in regulation of alternative splicing.},
url = {http://dx.doi.org/10.1016/j.str.2011.09.020},
volume = {19},
year = {2011}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The polypyrimidine tract-binding protein (PTB) is an important regulator of alternative splicing. PTB-regulated splicing of α-tropomyosin is enhanced by Raver1, a protein with four PTB-Raver1 interacting motifs (PRIs) that bind to the helical face of the second RNA recognition motif (RRM2) in PTB. We present the crystal structures of RRM2 in complex with PRI3 and PRI4 from Raver1, which--along with structure-based mutagenesis--reveal the molecular basis of their differential binding. High-affinity binding by Raver1 PRI3 involves shape-matched apolar contacts complemented by specific hydrogen bonds, a new variant of an established mode of peptide-RRM interaction. Our results refine the sequence of the PRI motif and place important structural constraints on functional models of PTB-Raver1 interactions. Our analysis indicates that the observed Raver1-PTB interaction is a general mode of binding that applies to Raver1 complexes with PTB paralogues such as nPTB and to complexes of Raver2 with PTB.
AU - Joshi,A
AU - Coelho,MB
AU - Kotik-Kogan,O
AU - Simpson,PJ
AU - Matthews,SJ
AU - Smith,CW
AU - Curry,S
DO - 10.1016/j.str.2011.09.020
EP - 1825
PY - 2011///
SP - 1816
TI - Crystallographic analysis of polypyrimidine tract-binding protein-Raver1 interactions involved in regulation of alternative splicing.
T2 - Structure
UR - http://dx.doi.org/10.1016/j.str.2011.09.020
UR - http://hdl.handle.net/10044/1/9331
VL - 19
ER -