Imperial College London
Alternate

Professor Steve Gentleman

Faculty of Medicine, Department of Brain Sciences

Professor of Neuropathology
 
 
 
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Contact

 

+44 (0)20 7594 6586s.gentleman Website

 
 
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Location

 

E407Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lai:2018:10.1038/s41467-018-03359-w,
author = {Lai, HM and Liu, KL and Ng, HHM and Goldfinger, M and Chau, TW and DeFelice, J and Tilley, B and Wong, WM and Wu, W and Gentleman, SM},
doi = {10.1038/s41467-018-03359-w},
journal = {Nature Communications},
title = {Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues},
url = {http://dx.doi.org/10.1038/s41467-018-03359-w},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Modern clearing techniques for the three-dimensional (3D) visualization of neural tissue microstructure have been very effective when used on rodent brain but very few studies haveutilized them on human brain material, mainly due to the inherent difficulties in processing post26mortem tissue. Here, we develop a tissue clearing solution, OPTIClear, optimised for fresh and archival human brain tissue, including formalin-fixed paraffin-embedded material. In light ofpractical challenges with immunostaining in tissue clearing, we adapt the use of cresyl violet for visualization of neurons in cleared tissue, with the potential for 3D quantification in regions ofinterest. Furthermore, we use lipophilic tracers for tracing of neuronal processes in post-mortem tissue, enabling the study of the morphology of human dendritic spines in 3D. The development ofthese different strategies for human tissue clearing has wide applicability and, we hope, will provide a baseline for further technique development.
AU  - Lai,HM
AU  - Liu,KL
AU  - Ng,HHM
AU  - Goldfinger,M
AU  - Chau,TW
AU  - DeFelice,J
AU  - Tilley,B
AU  - Wong,WM
AU  - Wu,W
AU  - Gentleman,SM
DO  - 10.1038/s41467-018-03359-w
PY  - 2018///
SN  - 2041-1723
TI  - Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-018-03359-w
UR  - http://hdl.handle.net/10044/1/57921
VL  - 9
ER  -
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