Publications
205 results found
Cloke B, Shah K, Kaneda H, et al., 2010, The Poly(C)-binding protein PCBP1 regulates the expression of the androgen receptor in decidualizing human endometrial stromal cells, 26th Annual Meeting of ESHRE, Publisher: OXFORD UNIV PRESS, Pages: I70-I71, ISSN: 0268-1161
Saloniemi T, Jarvensivu P, Koskimies P, et al., 2010, Novel Hydroxysteroid (17β) Dehydrogenase 1 Inhibitors Reverse Estrogen-Induced Endometrial Hyperplasia in Transgenic Mice, AMERICAN JOURNAL OF PATHOLOGY, Vol: 176, Pages: 1443-1451, ISSN: 0002-9440
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- Citations: 37
Myatt SS, Wang J, Monteiro LJ, et al., 2010, Definition of microRNAs that repress expression of the tumor suppressor gene FOXO1 in endometrial cancer., Cancer Research, Vol: 70, Pages: 367-377
Endometrial cancer is the most common malignancy of the lower female reproductive tract. The tumor suppressor FOXO1 is downregulated in endometrial cancer compared with normal endometrium but the underlying mechanisms are not well understood. Using microRNA (miR) target prediction algorithms, we identified several miRs that potentially bind the 3'-untranslated region of FOXO1 transcripts. Expression profiling of normal and malignant endometrial samples by quantitative real-time PCR and Northern blot analysis revealed an inverse correlation between the levels of FOXO1 protein and the abundance of several of the in silico-predicted miRs, suggesting that loss of FOXO1 expression in endometrial cancer may be mediated by miRs. To determine the role of candidate miRs, we used the endometrial cancer cell lines HEC-1B and Ishikawa, which express FOXO1 at high and low levels, respectively. Expression of miR-9, miR-27, miR-96, miR-153, miR-182, miR-183, or miR-186, but not miR-29a, miR-128, miR-152, or miR-486 mimetics in HEC-1B cells was sufficient to significantly reduce the abundance of FOXO1. Conversely, FOXO1 expression was efficiently restored in the Ishikawa cell line upon simultaneous inhibition of miR-9, miR-27, miR-96, miR-153, miR-183, and miR-186. Moreover, induction of FOXO1 in Ishikawa cells by miR inhibitors was accompanied by G1 cell cycle arrest and cell death, and was attenuated by the small interfering RNA-mediated downregulation of FOXO1 expression. Our findings identify several miRs overexpressed in endometrial cancer that function in concert to repress FOXO1 expression. Further, aberrant miR expression results in deregulated cell cycle control and impaired apoptotic responses, and thus, may be central to endometrial tumorigenesis.
Butler JS, Milliken DA, Dina R, et al., 2010, Isolated groin recurrence in vulval squamous cell cancer (VSCC). The importance of node count., European Journal of gynaecological Oncology, Vol: 5, Pages: 510-513
Ghaem-Maghami S, 2010, Gynaecological oncology., J Obstet Gynaecol, Vol: 30, ISSN: 1364-6893
Cloke B, Shah K, Kaneda H, et al., 2010, The poly(c)-binding protein-1 regulates expression of the androgen receptor, Endocrinology, Vol: 151, Pages: 3954-3964, ISSN: 1945-7170
The androgen receptor (AR) is a ligand-dependent transcription factor, expressed in male and female reproductive organs, and essential for normal reproduction in both sexes. The levels of AR are tightly controlled in androgen-responsive cells in which it plays a central role in the regulation of target gene expression. The AR is abundantly expressed in human endometrial stromal cells (HESCs), but levels decline markedly after differentiation into decidual cells in vivo and in primary cultures. Decidualization profoundly down-regulated AR protein levels with no discernible effect on either AR mRNA or protein stability, suggesting that loss of the receptor was a consequence of translational inhibition. Here we show that HESCs express three RNA-binding proteins, Hu antigen R and the poly(C)-binding proteins PCBP1 and PCBP2, that reportedly target the 3'-untranslated region of AR transcripts. Only PCBP1 expression was enhanced in secretory endometrium in vivo and in decidualizing HESCs. Furthermore, knockdown of PCBP1 in decidualizing cells was sufficient to restore AR protein levels, indicating that loss of the AR protein is primarily the consequence of a translational block. PCBP1 also blocked AR translation in a cell-free system, although this did not require binding to the 3'-untranslated region of the receptor mRNA. Furthermore, knockdown of PCBP1 in the prostate cancer LNCaP cell line also increased AR protein. Therefore, PCBP1 plays a major role in the dynamic expression of AR in both male and female androgen-responsive cells.
Koh KR, Newton RC, Ghaem-Maghami S, et al., 2010, Characterising Ovarian Cancer Morphology and Response to Chemotherapy using Fluorescence Confocal Endomicroscopy, IEEE Photonics Society Winter Topicals Meeting Series, Publisher: IEEE, Pages: 85-+
Soutter WP, Diakomanolis E, Lyons D, et al., 2009, Dynamic Spectral Imaging: Improving Colposcopy, CLINICAL CANCER RESEARCH, Vol: 15, Pages: 1814-1820, ISSN: 1078-0432
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- Citations: 51
Brownlow N, Mol C, Hayford C, et al., 2009, Dasatinib is a potent inhibitor of tumour-associated macrophages, osteoclasts and the FMS receptor, LEUKEMIA, Vol: 23, Pages: 590-594, ISSN: 0887-6924
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- Citations: 56
Ghaem-Maghami S, Soutter WP, 2009, Effect of margin status on cervical intraepithelial neoplasia recurrence following LLETZ in women over 50 years, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 116, Pages: 465-465, ISSN: 1470-0328
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- Citations: 2
Tuthill MH, Borley J, Gabra H, et al., 2008, Patterns of relapse in patients treated with surgery followed by platinum based chemotherapy for advanced epithelial ovarian cancer, JOURNAL OF CLINICAL ONCOLOGY, Vol: 26, ISSN: 0732-183X
Butler JS, Blake P, Bridges JE, et al., 2008, Outcomes of patients with recurrent vulval squamous cell cancer, GYNECOLOGIC ONCOLOGY, Vol: 108, Pages: S139-S139, ISSN: 0090-8258
Goto T, Takano M, Albergaria A, et al., 2008, Mechanism and functional consequences of loss of FOXO1 expression in endometrioid endometrial cancer cells, ONCOGENE, Vol: 27, Pages: 9-19, ISSN: 0950-9232
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- Citations: 89
Ghaem-Maghami S, Sagi S, Majeed G, et al., 2007, Incomplete excision of cervical intralepithelial neoplasia and risk of treatment failure: a meta-analysis, LANCET ONCOLOGY, Vol: 8, Pages: 985-993, ISSN: 1470-2045
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- Citations: 172
Ghaem-Maghami S, Cook H, Bird A, et al., 2006, High myopia and pre-eclampsia: a blinding combination, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 113, Pages: 608-609, ISSN: 1470-0328
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- Citations: 5
Ghaem-Maghami S, Brockbank E, Bridges J, 2006, Survey of surgical experience during training in obstetrics and gynaecology in the UK., J Obstet Gynaecol, Vol: 26, Pages: 297-301, ISSN: 0144-3615
The 2002 RCOG survey of training reported that the percentage of obstetric and gynaecology trainees who class their operative training as good or very good has declined from 45% in 1995, to 39% in 2002; reduction in years of training and number of working hours may have further impact on the surgical experience. In this study, we have attempted to assess the level of surgical confidence reported by senior and recently accredited trainees in obstetrics and gynaecology in the UK via an anonymised postal questionnaire. A total of 103 replies were received from 202 questionnaires. Some 99% of the respondents said they felt competent to carry out a simple total abdominal hysterectomy; 61.2% could confidently dissect the ureter and 55.3% could repair major damage to the bladder. However, when managing major obstetric haemorrhage, only 44.6% of respondents felt confident to perform a caesarean hysterectomy; 27.1% could dissect the ureter and 41.7% could apply a B-Lynch suture to the uterus. The level of competence increased with seniority and also with additional time spent in research, subspecialty training or other specialties. There appears to be an appropriate level of confidence in carrying out gynaecological surgical procedures by senior trainees and new consultants. However, surprisingly few respondents were confident in performing any surgical procedure necessary in the management of major obstetric haemorrhage. This may have serious implications in the provision of out of hours senior cover for maternity units in the future.
Ghaem-Maghami S, Nikolakopoulou Z, Gabra H, et al., 2005, WT1 as a target for immunotherapy inovarian cancer, IMMUNOLOGY, Vol: 116, Pages: 40-40, ISSN: 0019-2805
Brockbank EC, Ghaem-Maghami S, Bridges JE, 2004, The gynaecological management of women on tamoxifen: a national questionnaire survey., J Obstet Gynaecol, Vol: 24, Pages: 675-679, ISSN: 0144-3615
Tamoxifen is the standard adjuvant treatment for women with breast carcinoma, decreasing the incidence of contralateral disease. However, the risk of endometrial cancer is increased. To establish current gynaecological management of women receiving tamoxifen in the United Kingdom we conducted a postal questionnaire of consultant gynaecologists, enquiring about frequency of, and methods used to investigate women on tamoxifen. Ninety-five per cent investigate women on tamoxifen only if they are symptomatic. Pelvic ultrasound and endometrial sampling are used for first-line investigation by 68.7%. Interpreting ultrasound findings, endometrial thickness is the parameter regarded as most important. An endometrial thickness of greater than 5 mm is regarded abnormal by 47.8% of respondents and of 4 mm by 23.6%. As there is no consensus of opinion regarding normal values for endometrial thickness, further data are required to ensure consistency when interpreting ultrasound reports of women on tamoxifen.
Ghaem-Maghami S, Orton K, Soutter P, 2003, Key Advances in the Clinical Management of Ovarian Cancer, London, Publisher: Royal Society of Medicine Press, ISBN: 9781853155598
Ghaem-Maghami S, Ratti G, Ghaem-Maghami M, et al., 2003, Mucosal and systemic immune responses to plasmid protein pgp3 in patients with genital and ocular <i>Chlamydia trachomatis</i> infection, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, Vol: 132, Pages: 436-442, ISSN: 0009-9104
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- Citations: 23
Knapp MS, 2001, Using clinical evidence. Randomised controlled trials are not the only evidence., BMJ, Vol: 323, ISSN: 0959-8138
Knapp MS, 2001, Using clinical evidence - Randomised controlled trials are not the only evidence, BRITISH MEDICAL JOURNAL, Vol: 323, Pages: 165-165, ISSN: 0959-535X
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- Citations: 1
Penn Z, Ghaem-Maghami S, 2001, Indications for caesarean section, BEST PRACTICE & RESEARCH CLINICAL OBSTETRICS & GYNAECOLOGY, Vol: 15, Pages: 1-15, ISSN: 1521-6934
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- Citations: 78
Ghaem-Maghami S, Lewis DJM, 1999, <i>Chlamydia trachomatis</i>:: the role of cellular and humoral immune mechanisms in the development of blindness, CURRENT OPINION IN INFECTIOUS DISEASES, Vol: 12, Pages: 229-233, ISSN: 0951-7375
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- Citations: 1
GhaemMaghami S, Bailey RL, Mabey DCW, et al., 1997, Characterization of B-cell responses to Chlamydia trachomatis antigens in humans with trachoma, INFECTION AND IMMUNITY, Vol: 65, Pages: 4958-4964, ISSN: 0019-9567
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- Citations: 14
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