Imperial College London

Dr Sophie Helaine

Faculty of MedicineDepartment of Medicine

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3976s.helaine

 
 
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Location

 

2.20Flowers buildingSouth Kensington Campus

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Summary

 

Summary

Bacterial pathogens cause many diseases in humans and are frequently well controlled by treatment with antibiotics. However, antibiotics are increasingly becoming inefficient. In addition to the well-documented cases of antibiotic resistance, persistence, characterised by relapsing infections following antibiotic treatment, is a major problem. It has been discovered recently that for many bacterial species, a proportion of bacterial cells grown in laboratory medium can enter a dormant-like state in which they are not affected by antibiotics. These bacteria are called persisters. It is thought that eventually (sometimes decades after antibiotic treatment), persisters can resume growth, accounting for relapses of infection. Salmonella is the causative agent of various diseases, ranging from gastro-enteritis to typhoid fever. We have recently discovered that upon infection of host cells, there is a dramatic increase in the proportion of the Salmonella population that forms persisters. A family of genes, named Toxin/Antitoxin modules, is known to be involved in the formation of persisters in a non-pathogenic bacterial species, but almost nothing is known about these genes in pathogenic bacteria like Salmonella.  I recently started investigating their function, particularly in relation to persistence of Salmonella to antibiotics during infection. Understanding mechanisms of action of such genes could provide ways to prevent bacteria from becoming persisters, or force them out of that state so they become re-sensitised to antibiotics.

After completing my Ph.D. at Universite Paris 5- Necker, Paris, France, in 2006 with Dr Vladimir Pelicic, I joined the CMBI in the Department of  Medicine at Imperial College London as a Research Associate in the laboratory of Prof David Holden in 2007. I have been awarded a Junior Research Fellowship by Imperial College London in 2012 and started my own research group to study the formation and biology of Salmonella persisters during infection of the host. Our work allowed me to be granted an MRC Career Development Award from January 2015.

Publications

Journals

Fisher RA, Gollan B, Helaine S, 2017, Persistent bacterial infections and persister cells, Nature Reviews Microbiology, Vol:15, ISSN:1740-1526, Pages:453-464

Hall AMJ, Gollan B, Helaine S, 2017, Toxin-antitoxin systems: reversible toxicity, Current Opinion in Microbiology, Vol:36, ISSN:1369-5274, Pages:102-110

Saliba A-E, Li L, Westermann AJ, et al., 2017, Single-cell RNA-seq ties macrophage polarization to growth rate of intracellular Salmonella, Nature Microbiology, Vol:2, ISSN:2058-5276

Cheverton AM, Gollan B, Przydacz M, et al., 2016, A Salmonella Toxin Promotes Persister Formation through Acetylation of tRNA., Molecular Cell, Vol:63, ISSN:1097-2765, Pages:86-96

Fisher RA, Cheverton AM, Helaine S, 2016, Analysis of Macrophage-Induced Salmonella Persisters., Methods Mol Biol, Vol:1333, Pages:177-187

More Publications