Imperial College London

Dr Sophie Helaine

Faculty of MedicineDepartment of Medicine

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3976s.helaine

 
 
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Location

 

2.20Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

21 results found

Fisher RA, Gollan B, Helaine S, 2017, Persistent bacterial infections and persister cells, NATURE REVIEWS MICROBIOLOGY, Vol: 15, Pages: 453-464, ISSN: 1740-1526

JOURNAL ARTICLE

Hall AMJ, Gollan B, Helaine S, 2017, Toxin-antitoxin systems: reversible toxicity, CURRENT OPINION IN MICROBIOLOGY, Vol: 36, Pages: 102-110, ISSN: 1369-5274

JOURNAL ARTICLE

Saliba A-E, Li L, Westermann AJ, Appenzeller S, Stapels DAC, Schulte LN, Helaine S, Vogel Jet al., 2017, Single-cell RNA-seq ties macrophage polarization to growth rate of intracellular Salmonella, NATURE MICROBIOLOGY, Vol: 2, ISSN: 2058-5276

JOURNAL ARTICLE

Cheverton AM, Gollan B, Przydacz M, Wong CT, Mylona A, Hare SA, Helaine Set al., 2016, A Salmonella Toxin Promotes Persister Formation through Acetylation of tRNA., Molecular cell, Vol: 63, Pages: 86-96, ISSN: 1097-2765

The recalcitrance of many bacterial infections to antibiotic treatment is thought to be due to the presence of persisters that are non-growing, antibiotic-insensitive cells. Eventually, persisters resume growth, accounting for relapses of infection. Salmonella is an important pathogen that causes disease through its ability to survive inside macrophages. After macrophage phagocytosis, a significant proportion of the Salmonella population forms non-growing persisters through the action of toxin-antitoxin modules. Here we reveal that one such toxin, TacT, is an acetyltransferase that blocks the primary amine group of amino acids on charged tRNA molecules, thereby inhibiting translation and promoting persister formation. Furthermore, we report the crystal structure of TacT and note unique structural features, including two positively charged surface patches that are essential for toxicity. Finally, we identify a detoxifying mechanism in Salmonella wherein peptidyl-tRNA hydrolase counteracts TacT-dependent growth arrest, explaining how bacterial persisters can resume growth.

JOURNAL ARTICLE

Fisher RA, Cheverton AM, Helaine S, 2016, Analysis of Macrophage-Induced Salmonella Persisters., Methods Mol Biol, Vol: 1333, Pages: 177-187

A small subpopulation of non-replicating, multidrug-tolerant bacteria is present within clonal populations of many bacterial species. Known as persisters, these bacteria are probably the cause of relapsing infections such as typhoid fever. Formation of non-growing Salmonella persisters is stimulated by macrophage phagocytosis. This chapter outlines methods to identify and study persisters resulting from interactions between bacterial pathogens and their hosts. We use their antibiotic tolerance for isolation and enumeration and developed a method to study the heterogeneity of growth within clonal populations through single-cell analysis.

JOURNAL ARTICLE

Mouton JM, Helaine S, Holden DW, Sampson SLet al., 2016, Elucidating population-wide mycobacterial replication dynamics at the single-cell level, MICROBIOLOGY-SGM, Vol: 162, Pages: 966-978, ISSN: 1350-0872

JOURNAL ARTICLE

Santos AJM, Durkin CH, Helaine S, Boucrot E, Holden DWet al., 2016, Clustered Intracellular Salmonella enterica Serovar Typhimurium Blocks Host Cell Cytokinesis, INFECTION AND IMMUNITY, Vol: 84, Pages: 2149-2158, ISSN: 0019-9567

JOURNAL ARTICLE

Figueira R, Brown DR, Ferreira D, Eldridge MJG, Burchell L, Pan Z, Helaine S, Wigneshweraraj Set al., 2015, Adaptation to sustained nitrogen starvation by Escherichia coli requires the eukaryote-like serine/threonine kinase YeaG, SCIENTIFIC REPORTS, Vol: 5, ISSN: 2045-2322

JOURNAL ARTICLE

Caly DL, Coulthurst SJ, An S-Q, Helaine S, Malone JG, Ryan RPet al., 2014, Communication, Cooperation, and Social Interactions: a Report from the Third Young Microbiologists Symposium on Microbe Signalling, Organisation, and Pathogenesis, JOURNAL OF BACTERIOLOGY, Vol: 196, Pages: 3527-3533, ISSN: 0021-9193

JOURNAL ARTICLE

Helaine S, Cheverton AM, Watson KG, Faure LM, Matthews SA, Holden DWet al., 2014, Internalization of Salmonella by Macrophages Induces Formation of Nonreplicating Persisters, SCIENCE, Vol: 343, Pages: 204-208, ISSN: 0036-8075

JOURNAL ARTICLE

Helaine S, Kugelberg E, 2014, Bacterial persisters: formation, eradication, and experimental systems, TRENDS IN MICROBIOLOGY, Vol: 22, Pages: 417-424, ISSN: 0966-842X

JOURNAL ARTICLE

Helaine S, Holden DW, 2013, Heterogeneity of intracellular replication of bacterial pathogens, CURRENT OPINION IN MICROBIOLOGY, Vol: 16, Pages: 184-191, ISSN: 1369-5274

JOURNAL ARTICLE

Thompson JA, Liu M, Helainet S, Holden DWet al., 2011, Contribution of the PhoP/Q regulon to survival and replication of Salmonella enterica serovar Typhimurium in macrophages, MICROBIOLOGY-SGM, Vol: 157, Pages: 2084-2093, ISSN: 1350-0872

JOURNAL ARTICLE

Brown DR, Helaine S, Carbonnelle E, Pelicic Vet al., 2010, Systematic Functional Analysis Reveals That a Set of Seven Genes Is Involved in Fine-Tuning of the Multiple Functions Mediated by Type IV Pili in Neisseria meningitidis, INFECTION AND IMMUNITY, Vol: 78, Pages: 3053-3063, ISSN: 0019-9567

JOURNAL ARTICLE

Helaine S, Thompson JA, Watson KG, Liu M, Boyle C, Holden DWet al., 2010, Dynamics of intracellular bacterial replication at the single cell level, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 3746-3751, ISSN: 0027-8424

JOURNAL ARTICLE

Thompson JA, Helaine S, Holden DW, 2010, The Salmonella-mouse interaction: a versatile model to study bacterial infection. Book chapter in "Bacterial virulence: basic principles, models and global approaches, Editors: Sansonetti

BOOK CHAPTER

Helaine S, Dyer DH, Nassif X, Pelicic V, Forest KTet al., 2007, 3D structure/function analysis of PiIX reveals how minor pilins can modulate the virulence properties of type IV pili, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 104, Pages: 15888-15893, ISSN: 0027-8424

JOURNAL ARTICLE

Carbonnelle E, Helaine S, Nassif X, Pelicic Vet al., 2006, A systematic genetic analysis in Neisseria meningitidis defines the Pil proteins required for assembly, functionality, stabilization and export of type IV pili, MOLECULAR MICROBIOLOGY, Vol: 61, Pages: 1510-1522, ISSN: 0950-382X

JOURNAL ARTICLE

Carbonnelle E, Helaine S, Prouvensier L, Nassif X, Pelicic Vet al., 2005, Type IV pilus biogenesis in Neisseria meningitidis: PilW is involved in a step occurring after pilus assembly, essential for fibre stability and function, MOLECULAR MICROBIOLOGY, Vol: 55, Pages: 54-64, ISSN: 0950-382X

JOURNAL ARTICLE

Helaine S, Carbonnelle E, Prouvensier L, Beretti JL, Nassif X, Pelicic Vet al., 2005, PilX, a pilus-associated protein essential for bacterial aggregation, is a key to pilus-facilitated attachment of Neisseria meningitidis to human cells, MOLECULAR MICROBIOLOGY, Vol: 55, Pages: 65-77, ISSN: 0950-382X

JOURNAL ARTICLE

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