Imperial College London

Prof Steve Matthews

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Chemical and Structural Biology
 
 
 
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Contact

 

+44 (0)20 7594 5315s.j.matthews Website

 
 
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Location

 

602Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

205 results found

Andreasen M, Meisl G, Taylor JD, Michaels TCT, Levin A, Otzen DE, Chapman MR, Dobson CM, Matthews SJ, Knowles TPJet al., 2019, Physical Determinants of Amyloid Assembly in Biofilm Formation., MBio, Vol: 10

A wide range of bacterial pathogens have been shown to form biofilms, which significantly increase their resistance to environmental stresses, such as antibiotics, and are thus of central importance in the context of bacterial diseases. One of the major structural components of these bacterial biofilms are amyloid fibrils, yet the mechanism of fibril assembly and its importance for biofilm formation are currently not fully understood. By studying fibril formation in vitro, in a model system of two common but unrelated biofilm-forming proteins, FapC from Pseudomonas fluorescens and CsgA from Escherichia coli, we found that the two proteins have a common aggregation mechanism. In both systems, fibril formation proceeds via nucleated growth of linear fibrils exhibiting similar measured rates of elongation, with negligible fibril self-replication. These similarities between two unrelated systems suggest that convergent evolution plays a key role in tuning the assembly kinetics of functional amyloid fibrils and indicates that only a narrow window of mechanisms and assembly rates allows for successful biofilm formation. Thus, the amyloid assembly reaction is likely to represent a means for controlling biofilm formation, both by the organism and by possible inhibitory drugs.IMPORTANCE Biofilms are generated by bacteria, embedded in the formed extracellular matrix. The biofilm's function is to improve the survival of a bacterial colony through, for example, increased resistance to antibiotics or other environmental stresses. Proteins secreted by the bacteria act as a major structural component of this extracellular matrix, as they self-assemble into highly stable amyloid fibrils, making the biofilm very difficult to degrade by physical and chemical means once formed. By studying the self-assembly mechanism of the fibrils from their monomeric precursors in two unrelated bacteria, our experimental and theoretical approaches shed light on the mechanism of functional amyloid as

JOURNAL ARTICLE

Chorev DS, Baker LA, Wu D, Beilsten-Edmands V, Rouse SL, Zeev-Ben-Mordehai T, Jiko C, Samsudin F, Gerle C, Khalid S, Stewart AG, Matthews SJ, Gruenewald K, Robinson CVet al., 2018, Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry, SCIENCE, Vol: 362, Pages: 829-+, ISSN: 0036-8075

JOURNAL ARTICLE

Pakharukova N, McKenna S, Tuittila M, Paavilainen S, Malmi H, Xu Y, Parilova O, Matthews S, Zavialov AVet al., 2018, Archaic and alternative chaperones preserve pilin folding energy by providing incomplete structural information, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 293, Pages: 17070-17080, ISSN: 0021-9258

JOURNAL ARTICLE

Rouse SL, Stylianou F, Wu HYG, Berry J-L, Sewell L, Morgan RML, Sauerwein AC, Matthews Set al., 2018, The FapF Amyloid Secretion Transporter Possesses an Atypical Asymmetric Coiled Coil, JOURNAL OF MOLECULAR BIOLOGY, Vol: 430, Pages: 3863-3871, ISSN: 0022-2836

JOURNAL ARTICLE

Rouse SL, Matthews SJ, Dueholm MS, 2018, Ecology and Biogenesis of Functional Amyloids in Pseudomonas, JOURNAL OF MOLECULAR BIOLOGY, Vol: 430, Pages: 3685-3695, ISSN: 0022-2836

JOURNAL ARTICLE

Darvill N, Dubois DJ, Rouse SL, Hammoudi P-M, Blake T, Benjamin S, Liu B, Soldati-Favre D, Matthews Set al., 2018, Structural Basis of Phosphatidic Acid Sensing by APH in Apicomplexan Parasites, STRUCTURE, Vol: 26, Pages: 1059-+, ISSN: 0969-2126

JOURNAL ARTICLE

Tabib-Salazar A, Liu B, Barker D, Burchell L, Qimron U, Matthews SJ, Wigneshwerararj Set al., 2018, T7 phage factor required for managing RpoS in Escherichia coli, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 115, Pages: E5353-E5362, ISSN: 0027-8424

JOURNAL ARTICLE

Liu B, Wang Z, Lan L, Yang Q, Zhang P, Shi L, Lang Y, Tabib-Salazar A, Wigneshweraraj S, Zhang J, Wang Y, Tang Y, Matthews S, Zhang Xet al., 2018, A Rapid Colorimetric Method to Visualize Protein Interactions, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 24, Pages: 6727-6731, ISSN: 0947-6539

JOURNAL ARTICLE

Gatta AT, Sauerwein AC, Zhuravleva A, Levine TP, Matthews Set al., 2018, Structural insights into a StART-like domain in Lam4 and its interaction with sterol ligands, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 495, Pages: 2270-2274, ISSN: 0006-291X

JOURNAL ARTICLE

Rouse SL, Hawthorne WJ, Berry J-L, Chorev DS, Ionescu SA, Lambert S, Stylianou F, Ewert W, Mackie U, Morgan RML, Otzen D, Herbst F-A, Nielsen PH, Dueholm M, Bayley H, Robinson CV, Hare S, Matthews Set al., 2017, A new class of hybrid secretion system is employed in Pseudomonas amyloid biogenesis, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723

JOURNAL ARTICLE

Tabib-Salazar A, Liu B, Shadrin A, Burchell L, Wang Z, Wang Z, Goren MG, Yosef I, Qimron U, Severinov K, Matthews SJ, Wigneshweraraj Set al., 2017, Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein, NUCLEIC ACIDS RESEARCH, Vol: 45, Pages: 7697-7707, ISSN: 0305-1048

JOURNAL ARTICLE

Otzen DE, Vad BS, Dueholm MS, Nielsen PH, Rouse SL, Matthews SJet al., 2017, Self-organizing amyloid in bacteria, 19th IUPAB Congress / 11th EBSA Congress, Publisher: SPRINGER, Pages: S341-S341, ISSN: 0175-7571

CONFERENCE PAPER

Otzen DE, Vad BS, Dueholm MS, Nielsen PH, Rouse SL, Matthews SJet al., 2017, Self-organizing amyloid in bacteria, 19th IUPAB Congress / 11th EBSA Congress, Publisher: SPRINGER, Pages: S98-S98, ISSN: 0175-7571

CONFERENCE PAPER

Jia Y, Benjamin S, Liu Q, Xu Y, Dogga SK, Liu J, Matthews S, Soldati-Favre Det al., 2017, "Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold" (vol 1865, pg 208, 2017), BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1865, Pages: 631-631, ISSN: 1570-9639

JOURNAL ARTICLE

Jonsson R, Liu B, Struve C, Yang Y, Jorgensen R, Xu Y, Jenssen H, Krogfelt KA, Matthews Set al., 2017, Structural and functional studies of Escherichia coli aggregative adherence fimbriae (AAF/V) reveal a deficiency in extracellular matrix binding, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1865, Pages: 304-311, ISSN: 1570-9639

JOURNAL ARTICLE

Wang S-T, Lin Y, Todorova N, Xu Y, Mazo M, Rana S, Leonardo V, Amdursky N, Spicer CD, Alexander BD, Edwards AA, Matthews SJ, Yarovsky I, Stevens MMet al., 2017, Facet-Dependent Interactions of Islet Amyloid Polypeptide with Gold Nanoparticles: Implications for Fibril Formation and Peptide-Induced Lipid Membrane Disruption, CHEMISTRY OF MATERIALS, Vol: 29, Pages: 1550-1560, ISSN: 0897-4756

JOURNAL ARTICLE

Rouse SL, Hawthorne W, Berry J, Matthews Set al., 2017, Structural and Mechanistic Insights into Transport of Functional Amyloid Subunits across the Pseudomonas Outer Membrane, 61st Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 188A-188A, ISSN: 0006-3495

CONFERENCE PAPER

Jia Y, Benjamin S, Liu Q, Xu Y, Dogga SK, Liu J, Matthews S, Soldati-Favre Det al., 2017, Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1865, Pages: 208-219, ISSN: 1570-9639

JOURNAL ARTICLE

Hawthorne W, Rouse S, Sewell L, Matthews SJet al., 2016, Structural insights into functional amyloid inhibition in Gram -ve bacteria, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 44, Pages: 1643-1649, ISSN: 0300-5127

JOURNAL ARTICLE

Rouse SL, Hawthorne WJ, Lambert S, Morgan ML, Hare SA, Matthews Set al., 2016, Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 72, Pages: 892-896, ISSN: 2053-230X

JOURNAL ARTICLE

Rasheed M, Garnett J, Perez-Dorado I, Muhl D, Filloux A, Matthews Set al., 2016, Crystal structure of the CupB6 adhesive tip from the chaperone-usher family of pili from Pseudomonas aeruginosa, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1864, Pages: 1500-1505, ISSN: 1570-9639

JOURNAL ARTICLE

Brown DR, Sheppard CM, Burchell L, Matthews S, Wigneshweraraj Set al., 2016, The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism, JOURNAL OF MOLECULAR BIOLOGY, Vol: 428, Pages: 3911-3919, ISSN: 0022-2836

JOURNAL ARTICLE

Lee W-C, Matthews S, Garnett JA, 2016, Crystal structure and analysis of HdaB: The enteroaggregative Escherichia coli AAF/IV pilus tip protein, PROTEIN SCIENCE, Vol: 25, Pages: 1898-1905, ISSN: 0961-8368

JOURNAL ARTICLE

Liang X, Liu B, Zhu F, Scannapieco FA, Haase EM, Matthews S, Wu Het al., 2016, A distinct sortase SrtB anchors and processes a streptococcal adhesin AbpA with a novel structural property, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Grundy GJ, Polo LM, Zeng Z, Rulten SL, Hoch NC, Paomephan P, Xu Y, Sweet SM, Thorne AW, Oliver AW, Matthews SJ, Pearl LH, Caldecott KWet al., 2016, PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2), NATURE COMMUNICATIONS, Vol: 7, ISSN: 2041-1723

JOURNAL ARTICLE

Berry J-L, Xu Y, Ward PN, Lea SM, Matthews SJ, Pelicic Vet al., 2016, A Comparative Structure/Function Analysis of Two Type IV Pilin DNA Receptors Defines a Novel Mode of DNA Binding, STRUCTURE, Vol: 24, Pages: 926-934, ISSN: 0969-2126

JOURNAL ARTICLE

Taglialegna A, Navarro S, Ventura S, Garnett JA, Matthews S, Penades JR, Lasa I, Valle Jet al., 2016, Staphylococcal Bap Proteins Build Amyloid Scaffold Biofilm Matrices in Response to Environmental Signals, PLOS PATHOGENS, Vol: 12, ISSN: 1553-7366

JOURNAL ARTICLE

Taylor JD, Hawthorne WJ, Lo J, Dear A, Jain N, Meisl G, Andreasen M, Fletcher C, Koch M, Darvill N, Scull N, Escalera-Maurer A, Sefer L, Wenman R, Lambert S, Jean J, Xu Y, Turner B, Kazarian SG, Chapman MR, Bubeck D, de Simone A, Knowles TPJ, Matthews SJet al., 2016, Electrostatically-guided inhibition of Curli amyloid nucleation by the CsgC-like family of chaperones, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Miliara X, Matthews S, 2016, Structural comparison of yeast and human intra-mitochondrial lipid transport systems, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 44, Pages: 479-485, ISSN: 0300-5127

JOURNAL ARTICLE

Taylor JD, Taylor G, Hare SA, Matthews SJet al., 2016, Structures of the DfsB Protein Family Suggest a Cationic, Helical Sibling Lethal Factor Peptide, JOURNAL OF MOLECULAR BIOLOGY, Vol: 428, Pages: 554-560, ISSN: 0022-2836

JOURNAL ARTICLE

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