Imperial College London

Prof Steve Matthews

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Chemical and Structural Biology
 
 
 
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Contact

 

+44 (0)20 7594 5315s.j.matthews Website

 
 
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Location

 

602Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

210 results found

Zhang P, Wang Z, Zhao S, Wang Y, Matthews S, Liu Bet al., 2019, 1H, 13C and 15N NMR assignments of Bacillus subtilis bacteriophage SPO1 protein Gp46., Biomol NMR Assign, Vol: 13, Pages: 245-247

Bacterial antibiotic resistance is a serious threat to public health and bacteriophage therapy is an alternative for antibiotics in the era of multidrug resistance. While phage draws attention in fighting bacterial infection and is used in protein display to study macromolecular interactions, the molecular machinery of the host invasion mechanism remains largely unclear for many bacteriophages. Despite recent studies on T4 and T7 phages of Gram-negative model organism Escherichia coli revealing many interesting features of their invasive strategies, the studies on Gram-positive bacterial phages still lag far behind their counterparts. SPO1 is a lytic phage of model organism Bacillus subtilis and one of the best studied Gram-positive bacterial phages. SPO1 features a unique Host Takeover Module coding for 24 proteins which show little similarity to any previously known proteins. Gp46, located in this module, is an acidic protein that is produced by SPO1 presumably during the host takeover event. Here we describe the complete resonance assignment of Gp46 as the basis for the first structure determination of SPO1 phage protein and further mechanism study.

JOURNAL ARTICLE

Miliara X, Tatsuta T, Berry J-L, Rouse SL, Solak K, Chorev DS, Wu D, Robinson CV, Matthews S, Langer Tet al., 2019, Structural determinants of lipid specificity within Ups/PRELI lipid transfer proteins, NATURE COMMUNICATIONS, Vol: 10, ISSN: 2041-1723

JOURNAL ARTICLE

Berry J-L, Gurung I, Anonsen JH, Spielman I, Harper E, Hall AMJ, Goosens VJ, Raynaud C, Koomey M, Biais N, Matthews S, Pelicic Vet al., 2019, Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacterium Streptococcus sanguinis., J Biol Chem

Type IV pili (Tfp) are functionally versatile filaments, widespread in prokaryotes, that belong to a large class of filamentous nanomachines known as type IV filaments (Tff). Although Tfp have been extensively studied in several Gram-negative pathogens where they function as key virulence factors, many aspects of their biology remain poorly understood. Here, we performed a global biochemical and structural analysis of Tfp in a recently emerged Gram-positive model, Streptococcus sanguinis In particular, we focused on the five pilins and pilin-like proteins involved in Tfp biology in S. sanguinis We found that the two major pilins, PilE1 and PilE2, (i) follow widely conserved principles for processing by the prepilin peptidase PilD and for assembly into filaments; (ii) display only one of the post-translational modifications frequently found in pilins, i.e. a methylated N-terminus; (iii) are found in the same hetero-polymeric filaments; and (iv) are not functionally equivalent. The 3D structure of PilE1, solved by NMR, revealed a classical pilin fold with a highly unusual flexible C-terminus. Intriguingly, PilE1 more closely resembles pseudopilins forming shorter Tff than bona fide Tfp-forming major pilins, underlining the evolutionary relatedness among different Tff. Finally, we show that S. sanguinis Tfp contain a low abundance of three additional proteins processed by PilD, the minor pilins PilA, PilB, and PilC. These findings provide the first global biochemical and structural picture of a Gram-positive Tfp and have fundamental implications for our understanding of a widespread class of filamentous nanomachines.

JOURNAL ARTICLE

Tabib-Salazar A, Mulvenna N, Severinov K, Matthews SJ, Wigneshweraraj Set al., 2019, Xenogeneic Regulation of the Bacterial Transcription Machinery., J Mol Biol

The parasitic life cycle of viruses involves the obligatory subversion of the host's macromolecular processes for efficient viral progeny production. Viruses that infect bacteria, bacteriophages (phages), are no exception and have evolved sophisticated ways to control essential biosynthetic machineries of their bacterial prey to benefit phage development. The xenogeneic regulation of bacterial cell function is a poorly understood area of bacteriology. The activity of the bacterial transcription machinery, the RNA polymerase (RNAP), is often regulated by a variety of mechanisms involving small phage-encoded proteins. In this review, we provide a brief overview of known phage proteins that interact with the bacterial RNAP and compare how two prototypical phages of Escherichia coli, T4 and T7, use small proteins to "puppeteer" the bacterial RNAP to ensure a successful infection.

JOURNAL ARTICLE

Polo LM, Xu Y, Hornyak P, Garces F, Zeng Z, Hailstone R, Matthews SJ, Caldecott KW, Oliver AW, Pearl LHet al., 2019, Efficient Single-Strand Break Repair Requires Binding to Both Poly(ADP-Ribose) and DNA by the Central BRCT Domain of XRCC1, CELL REPORTS, Vol: 26, Pages: 573-+, ISSN: 2211-1247

JOURNAL ARTICLE

Andreasen M, Meisl G, Taylor JD, Michaels TCT, Levin A, Otzen DE, Chapman MR, Dobson CM, Matthews SJ, Knowles TPJet al., 2019, Physical Determinants of Amyloid Assembly in Biofilm Formation, MBIO, Vol: 10, ISSN: 2150-7511

JOURNAL ARTICLE

Chorev DS, Baker LA, Wu D, Beilsten-Edmands V, Rouse SL, Zeev-Ben-Mordehai T, Jiko C, Samsudin F, Gerle C, Khalid S, Stewart AG, Matthews SJ, Gruenewald K, Robinson CVet al., 2018, Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry, SCIENCE, Vol: 362, Pages: 829-+, ISSN: 0036-8075

JOURNAL ARTICLE

Pakharukova N, McKenna S, Tuittila M, Paavilainen S, Malmi H, Xu Y, Parilova O, Matthews S, Zavialov AVet al., 2018, Archaic and alternative chaperones preserve pilin folding energy by providing incomplete structural information, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 293, Pages: 17070-17080, ISSN: 0021-9258

JOURNAL ARTICLE

Rouse SL, Stylianou F, Wu HYG, Berry J-L, Sewell L, Morgan RML, Sauerwein AC, Matthews Set al., 2018, The FapF Amyloid Secretion Transporter Possesses an Atypical Asymmetric Coiled Coil, JOURNAL OF MOLECULAR BIOLOGY, Vol: 430, Pages: 3863-3871, ISSN: 0022-2836

JOURNAL ARTICLE

Rouse SL, Matthews SJ, Dueholm MS, 2018, Ecology and Biogenesis of Functional Amyloids in Pseudomonas, JOURNAL OF MOLECULAR BIOLOGY, Vol: 430, Pages: 3685-3695, ISSN: 0022-2836

JOURNAL ARTICLE

Darvill N, Dubois DJ, Rouse SL, Hammoudi P-M, Blake T, Benjamin S, Liu B, Soldati-Favre D, Matthews Set al., 2018, Structural Basis of Phosphatidic Acid Sensing by APH in Apicomplexan Parasites, STRUCTURE, Vol: 26, Pages: 1059-+, ISSN: 0969-2126

JOURNAL ARTICLE

Tabib-Salazar A, Liu B, Barker D, Burchell L, Qimron U, Matthews SJ, Wigneshwerararj Set al., 2018, T7 phage factor required for managing RpoS in Escherichia coli, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 115, Pages: E5353-E5362, ISSN: 0027-8424

JOURNAL ARTICLE

Liu B, Wang Z, Lan L, Yang Q, Zhang P, Shi L, Lang Y, Tabib-Salazar A, Wigneshweraraj S, Zhang J, Wang Y, Tang Y, Matthews S, Zhang Xet al., 2018, A Rapid Colorimetric Method to Visualize Protein Interactions, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 24, Pages: 6727-6731, ISSN: 0947-6539

JOURNAL ARTICLE

Gatta AT, Sauerwein AC, Zhuravleva A, Levine TP, Matthews Set al., 2018, Structural insights into a StART-like domain in Lam4 and its interaction with sterol ligands, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 495, Pages: 2270-2274, ISSN: 0006-291X

JOURNAL ARTICLE

Rouse SL, Hawthorne WJ, Berry J-L, Chorev DS, Ionescu SA, Lambert S, Stylianou F, Ewert W, Mackie U, Morgan RML, Otzen D, Herbst F-A, Nielsen PH, Dueholm M, Bayley H, Robinson CV, Hare S, Matthews Set al., 2017, A new class of hybrid secretion system is employed in Pseudomonas amyloid biogenesis, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723

JOURNAL ARTICLE

Tabib-Salazar A, Liu B, Shadrin A, Burchell L, Wang Z, Wang Z, Goren MG, Yosef I, Qimron U, Severinov K, Matthews SJ, Wigneshweraraj Set al., 2017, Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein, NUCLEIC ACIDS RESEARCH, Vol: 45, Pages: 7697-7707, ISSN: 0305-1048

JOURNAL ARTICLE

Otzen DE, Vad BS, Dueholm MS, Nielsen PH, Rouse SL, Matthews SJet al., 2017, Self-organizing amyloid in bacteria, 19th IUPAB Congress / 11th EBSA Congress, Publisher: SPRINGER, Pages: S341-S341, ISSN: 0175-7571

CONFERENCE PAPER

Otzen DE, Vad BS, Dueholm MS, Nielsen PH, Rouse SL, Matthews SJet al., 2017, Self-organizing amyloid in bacteria, 19th IUPAB Congress / 11th EBSA Congress, Publisher: SPRINGER, Pages: S98-S98, ISSN: 0175-7571

CONFERENCE PAPER

Jia Y, Benjamin S, Liu Q, Xu Y, Dogga SK, Liu J, Matthews S, Soldati-Favre Det al., 2017, "Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold" (vol 1865, pg 208, 2017), BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1865, Pages: 631-631, ISSN: 1570-9639

JOURNAL ARTICLE

Jonsson R, Liu B, Struve C, Yang Y, Jorgensen R, Xu Y, Jenssen H, Krogfelt KA, Matthews Set al., 2017, Structural and functional studies of Escherichia coli aggregative adherence fimbriae (AAF/V) reveal a deficiency in extracellular matrix binding, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1865, Pages: 304-311, ISSN: 1570-9639

JOURNAL ARTICLE

Wang S-T, Lin Y, Todorova N, Xu Y, Mazo M, Rana S, Leonardo V, Amdursky N, Spicer CD, Alexander BD, Edwards AA, Matthews SJ, Yarovsky I, Stevens MMet al., 2017, Facet-Dependent Interactions of Islet Amyloid Polypeptide with Gold Nanoparticles: Implications for Fibril Formation and Peptide-Induced Lipid Membrane Disruption, CHEMISTRY OF MATERIALS, Vol: 29, Pages: 1550-1560, ISSN: 0897-4756

JOURNAL ARTICLE

Rouse SL, Hawthorne W, Berry J, Matthews Set al., 2017, Structural and Mechanistic Insights into Transport of Functional Amyloid Subunits across the Pseudomonas Outer Membrane, 61st Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 188A-188A, ISSN: 0006-3495

CONFERENCE PAPER

Jia Y, Benjamin S, Liu Q, Xu Y, Dogga SK, Liu J, Matthews S, Soldati-Favre Det al., 2017, Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1865, Pages: 208-219, ISSN: 1570-9639

JOURNAL ARTICLE

Hawthorne W, Rouse S, Sewell L, Matthews SJet al., 2016, Structural insights into functional amyloid inhibition in Gram -ve bacteria, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 44, Pages: 1643-1649, ISSN: 0300-5127

JOURNAL ARTICLE

Rouse SL, Hawthorne WJ, Lambert S, Morgan ML, Hare SA, Matthews Set al., 2016, Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 72, Pages: 892-896, ISSN: 2053-230X

JOURNAL ARTICLE

Rasheed M, Garnett J, Perez-Dorado I, Muhl D, Filloux A, Matthews Set al., 2016, Crystal structure of the CupB6 adhesive tip from the chaperone-usher family of pili from Pseudomonas aeruginosa, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1864, Pages: 1500-1505, ISSN: 1570-9639

JOURNAL ARTICLE

Brown DR, Sheppard CM, Burchell L, Matthews S, Wigneshweraraj Set al., 2016, The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism, JOURNAL OF MOLECULAR BIOLOGY, Vol: 428, Pages: 3911-3919, ISSN: 0022-2836

JOURNAL ARTICLE

Lee W-C, Matthews S, Garnett JA, 2016, Crystal structure and analysis of HdaB: The enteroaggregative Escherichia coli AAF/IV pilus tip protein, PROTEIN SCIENCE, Vol: 25, Pages: 1898-1905, ISSN: 0961-8368

JOURNAL ARTICLE

Liang X, Liu B, Zhu F, Scannapieco FA, Haase EM, Matthews S, Wu Het al., 2016, A distinct sortase SrtB anchors and processes a streptococcal adhesin AbpA with a novel structural property, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Grundy GJ, Polo LM, Zeng Z, Rulten SL, Hoch NC, Paomephan P, Xu Y, Sweet SM, Thorne AW, Oliver AW, Matthews SJ, Pearl LH, Caldecott KWet al., 2016, PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2), NATURE COMMUNICATIONS, Vol: 7, ISSN: 2041-1723

JOURNAL ARTICLE

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