Imperial College London

Prof Steve Matthews

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Chemical and Structural Biology
 
 
 
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Contact

 

+44 (0)20 7594 5315s.j.matthews Website

 
 
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Location

 

Room 602Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

190 results found

Jia Y, Benjamin S, Liu Q, Xu Y, Dogga SK, Liu J, Matthews S, Soldati-Favre Det al., 2017, Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1865, Pages: 208-219, ISSN: 1570-9639

JOURNAL ARTICLE

Jonsson R, Liu B, Struve C, Yang Y, Jorgensen R, Xu Y, Jenssen H, Krogfelt KA, Matthews Set al., 2017, Structural and functional studies of Escherichia coli aggregative adherence fimbriae (AAF/V) reveal a deficiency in extracellular matrix binding, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1865, Pages: 304-311, ISSN: 1570-9639

JOURNAL ARTICLE

Rouse SL, Hawthorne W, Berry J, Matthews Set al., 2017, Structural and Mechanistic Insights into Transport of Functional Amyloid Subunits across the Pseudomonas Outer Membrane, 61st Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 188A-188A, ISSN: 0006-3495

CONFERENCE PAPER

Rouse SL, Hawthorne WJ, Berry J-L, Chorev DS, Ionescu SA, Lambert S, Stylianou F, Ewert W, Mackie U, Morgan RML, Otzen D, Herbst F-A, Nielsen PH, Dueholm M, Bayley H, Robinson CV, Hare S, Matthews Set al., 2017, A new class of hybrid secretion system is employed in Pseudomonas amyloid biogenesis, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723

JOURNAL ARTICLE

Tabib-Salazar A, Liu B, Shadrin A, Burchell L, Wang Z, Wang Z, Goren MG, Yosef I, Qimron U, Severinov K, Matthews SJ, Wigneshweraraj Set al., 2017, Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein, NUCLEIC ACIDS RESEARCH, Vol: 45, Pages: 7697-7707, ISSN: 0305-1048

JOURNAL ARTICLE

Wang S-T, Lin Y, Todorova N, Xu Y, Mazo M, Rana S, Leonardo V, Amdursky N, Spicer CD, Alexander BD, Edwards AA, Matthews SJ, Yarovsky I, Stevens MMet al., 2017, Facet-Dependent Interactions of Islet Amyloid Polypeptide with Gold Nanoparticles: Implications for Fibril Formation and Peptide-Induced Lipid Membrane Disruption, CHEMISTRY OF MATERIALS, Vol: 29, Pages: 1550-1560, ISSN: 0897-4756

JOURNAL ARTICLE

Berry J-L, Xu Y, Ward PN, Lea SM, Matthews SJ, Pelicic Vet al., 2016, A Comparative Structure/Function Analysis of Two Type IV Pilin DNA Receptors Defines a Novel Mode of DNA Binding, STRUCTURE, Vol: 24, Pages: 926-934, ISSN: 0969-2126

JOURNAL ARTICLE

Brown DR, Sheppard CM, Burchell L, Matthews S, Wigneshweraraj Set al., 2016, The Xp10 Bacteriophage Protein P7 Inhibits Transcription by the Major and Major Variant Forms of the Host RNA Polymerase via a Common Mechanism, JOURNAL OF MOLECULAR BIOLOGY, Vol: 428, Pages: 3911-3919, ISSN: 0022-2836

JOURNAL ARTICLE

Grundy GJ, Polo LM, Zeng Z, Rulten SL, Hoch NC, Paomephan P, Xu Y, Sweet SM, Thorne AW, Oliver AW, Matthews SJ, Pearl LH, Caldecott KWet al., 2016, PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2), NATURE COMMUNICATIONS, Vol: 7, ISSN: 2041-1723

JOURNAL ARTICLE

Hawthorne W, Rouse S, Sewell L, Matthews SJet al., 2016, Structural insights into functional amyloid inhibition in Gram -ve bacteria, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 44, Pages: 1643-1649, ISSN: 0300-5127

JOURNAL ARTICLE

Lee W-C, Matthews S, Garnett JA, 2016, Crystal structure and analysis of HdaB: The enteroaggregative Escherichia coli AAF/IV pilus tip protein, PROTEIN SCIENCE, Vol: 25, Pages: 1898-1905, ISSN: 0961-8368

JOURNAL ARTICLE

Leen EN, Sorgeloos F, Correia S, Chaudhry Y, Cannac F, Pastore C, Xu Y, Graham SC, Matthews SJ, Goodfellow IG, Curry Set al., 2016, A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation., PLOS Pathogens, Vol: 12, ISSN: 1553-7366

Translation initiation is a critical early step in the replication cycle of the positive-sense, single-stranded RNA genome of noroviruses, a major cause of gastroenteritis in humans. Norovirus RNA, which has neither a 5´ m7G cap nor an internal ribosome entry site (IRES), adopts an unusual mechanism to initiate protein synthesis that relies on interactions between the VPg protein covalently attached to the 5´-end of the viral RNA and eukaryotic initiation factors (eIFs) in the host cell. For murine norovirus (MNV) we previously showed that VPg binds to the middle fragment of eIF4G (4GM; residues 652-1132). Here we have used pull-down assays, fluorescence anisotropy, and isothermal titration calorimetry (ITC) to demonstrate that a stretch of ~20 amino acids at the C terminus of MNV VPg mediates direct and specific binding to the HEAT-1 domain within the 4GM fragment of eIF4G. Our analysis further reveals that the MNV C terminus binds to eIF4G HEAT-1 via a motif that is conserved in all known noroviruses. Fine mutagenic mapping suggests that the MNV VPg C terminus may interact with eIF4G in a helical conformation. NMR spectroscopy was used to define the VPg binding site on eIF4G HEAT-1, which was confirmed by mutagenesis and binding assays. We have found that this site is non-overlapping with the binding site for eIF4A on eIF4G HEAT-1 by demonstrating that norovirus VPg can form ternary VPg-eIF4G-eIF4A complexes. The functional significance of the VPg-eIF4G interaction was shown by the ability of fusion proteins containing the C-terminal peptide of MNV VPg to inhibit in vitro translation of norovirus RNA but not cap- or IRES-dependent translation. These observations define important structural details of a functional interaction between norovirus VPg and eIF4G and reveal a binding interface that might be exploited as a target for antiviral therapy.

JOURNAL ARTICLE

Liang X, Liu B, Zhu F, Scannapieco FA, Haase EM, Matthews S, Wu Het al., 2016, A distinct sortase SrtB anchors and processes a streptococcal adhesin AbpA with a novel structural property, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Miliara X, Matthews S, 2016, Structural comparison of yeast and human intra-mitochondrial lipid transport systems, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 44, Pages: 479-485, ISSN: 0300-5127

JOURNAL ARTICLE

Rasheed M, Garnett J, Perez-Dorado I, Muhl D, Filloux A, Matthews Set al., 2016, Crystal structure of the CupB6 adhesive tip from the chaperone-usher family of pili from Pseudomonas aeruginosa, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1864, Pages: 1500-1505, ISSN: 1570-9639

JOURNAL ARTICLE

Rouse SL, Hawthorne WJ, Lambert S, Morgan ML, Hare SA, Matthews Set al., 2016, Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 72, Pages: 892-896, ISSN: 2053-230X

JOURNAL ARTICLE

Taglialegna A, Navarro S, Ventura S, Garnett JA, Matthews S, Penades JR, Lasa I, Valle Jet al., 2016, Staphylococcal Bap Proteins Build Amyloid Scaffold Biofilm Matrices in Response to Environmental Signals, PLOS PATHOGENS, Vol: 12, ISSN: 1553-7366

JOURNAL ARTICLE

Taylor JD, Hawthorne WJ, Lo J, Dear A, Jain N, Meisl G, Andreasen M, Fletcher C, Koch M, Darvill N, Scull N, Escalera-Maurer A, Sefer L, Wenman R, Lambert S, Jean J, Xu Y, Turner B, Kazarian SG, Chapman MR, Bubeck D, de Simone A, Knowles TPJ, Matthews SJet al., 2016, Electrostatically-guided inhibition of Curli amyloid nucleation by the CsgC-like family of chaperones, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Taylor JD, Taylor G, Hare SA, Matthews SJet al., 2016, Structures of the DfsB Protein Family Suggest a Cationic, Helical Sibling Lethal Factor Peptide, JOURNAL OF MOLECULAR BIOLOGY, Vol: 428, Pages: 554-560, ISSN: 0022-2836

JOURNAL ARTICLE

Benjamin S, Williams F, Kerry L, Matthews Set al., 2015, NMR assignment of the immune mapped protein 1 (IMP1) homologue from Plasmodium falciparum, BIOMOLECULAR NMR ASSIGNMENTS, Vol: 9, Pages: 393-395, ISSN: 1874-2718

JOURNAL ARTICLE

Curry S, Leen EN, Sorgeloos F, Correia S, Chaudhry Y, Cannac F, Pastore C, Xu Y, Graham SC, Matthews SJ, Goodfellow IGet al., 2015, A conserved interaction between a C-terminal motif in Norovirus VPg and the HEAT-1 domain of eIF4G is essential for translation initiation, Publisher: bioRxiv

Translation initiation is a critical early step in the replication cycle of the positive-sense, single-stranded RNA genome of noroviruses, a major cause of gastroenteritis in humans. Norovirus RNA, which has neither a 5 ́ m7G cap nor an internal ribosome entry site (IRES), adopts an unusual mechanism to initiate protein synthesis that relies on interactions between the VPg protein covalently attached to the 5 ́-end of the viral RNA and eukaryotic initiation factors (eIFs) in the host cell. For murine norovirus (MNV) we previously showed that VPg binds to the middle fragment of eIF4G (4GM; residues 652-1132). Here we have used pull-down assays, fluorescence anisotropy, and isothermal titration calorimetry (ITC) to demonstrate that a stretch of ~20 amino acids at the C terminus of MNV VPg mediates direct and specific binding to the HEAT-1 domain within the 4GM fragment of eIF4G. Our analysis further reveals that the MNV C-terminus binds to eIF4G HEAT-1 via a motif that is conserved in all known noroviruses. Fine mutagenic mapping suggests that the MNV VPg C terminus may interact with eIF4G in a helical conformation. NMR spectroscopy was used to define the VPg binding site on eIF4G HEAT-1, which was confirmed by mutagenesis and binding assays. We have found that this site is non-overlapping with the binding site for eIF4A on eIF4G HEAT-1 by demonstrating that norovirus VPg can form ternary VPg-eIF4G-eIF4A complexes. The functional significance of the VPg-eIF4G interaction was shown by the ability of fusion proteins containing the C- terminal peptide of MNV VPg to inhibit translation of norovirus RNA but not cap- or IRES-dependent translation. These observations define important structural details of a functional interaction between norovirus VPg and eIF4G and reveal a binding interface that might be exploited as a target for antiviral therapy.

REPORT

Evans ML, Chorell E, Taylor JD, Aden J, Gotheson A, Li F, Koch M, Sefer L, Matthews SJ, Wittung-Stafshede P, Almqvist F, Chapman MRet al., 2015, The Bacterial Curli System Possesses a Potent and Selective Inhibitor of Amyloid Formation, MOLECULAR CELL, Vol: 57, Pages: 445-455, ISSN: 1097-2765

JOURNAL ARTICLE

Garnett JA, Diallo M, Matthews SJ, 2015, Purification, crystallization and preliminary X-ray diffraction analysis of the Escherichia coli common pilus chaperone EcpB, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 71, Pages: 676-679, ISSN: 2053-230X

JOURNAL ARTICLE

Garnett JA, Muhl D, Douse CH, Hui K, Busch A, Omisore A, Yang Y, Simpson P, Marchant J, Waksman G, Matthews S, Filloux Aet al., 2015, Structure-function analysis reveals that the Pseudomonas aeruginosa Tps4 two-partner secretion system is involved in CupB5 translocation, PROTEIN SCIENCE, Vol: 24, Pages: 670-687, ISSN: 0961-8368

JOURNAL ARTICLE

Huynh M-H, Liu B, Henry M, Liew L, Matthews SJ, Carruthers VBet al., 2015, Structural Basis of Toxoplasma gondii MIC2-associated Protein Interaction with MIC2, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 290, Pages: 1432-1441, ISSN: 0021-9258

JOURNAL ARTICLE

Liu B, Zhu F, Wu H, Matthews Set al., 2015, NMR assignment of the amylase-binding protein A from Streptococcus parasanguinis, BIOMOLECULAR NMR ASSIGNMENTS, Vol: 9, Pages: 173-175, ISSN: 1874-2718

JOURNAL ARTICLE

Miliara X, Garnett JA, Tatsuta T, Abid Ali F, Baldie H, Perez-Dorado I, Simpson P, Yague E, Langer T, Matthews Set al., 2015, Structural insight into the TRIAP1/PRELI-like domain family of mitochondrial phospholipid transfer complexes, EMBO REPORTS, Vol: 16, Pages: 824-835, ISSN: 1469-221X

JOURNAL ARTICLE

Pakharukova N, Garnett JA, Tuittila M, Paavilainen S, Diallo M, Xu Y, Matthews SJ, Zavialov AVet al., 2015, Structural Insight into Archaic and Alternative Chaperone-Usher Pathways Reveals a Novel Mechanism of Pilus Biogenesis, PLOS PATHOGENS, Vol: 11, ISSN: 1553-7366

JOURNAL ARTICLE

Pinzan CF, Sardinha-Silva A, Almeida F, Lai L, Lopes CD, Lourenco EV, Panunto-Castelo A, Matthews S, Roque-Barreira MCet al., 2015, Vaccination with Recombinant Microneme Proteins Confers Protection against Experimental Toxoplasmosis in Mice, PLOS ONE, Vol: 10, ISSN: 1932-6203

JOURNAL ARTICLE

Polo LM, Grundy G, Rulten S, Xu Y, Matthews S, Caldecot K, Oliver A, Pearl Let al., 2015, New structural insights into PARP3 function, 40th Congress of the Federation-of-European-Biochemical-Societies (FEBS) - The Biochemical Basis of Life, Publisher: WILEY-BLACKWELL, Pages: 397-397, ISSN: 1742-464X

CONFERENCE PAPER

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