Imperial College London
Alternate

Prof Steve Matthews

Faculty of Natural Sciences, Department of Life Sciences

Professor of Chemical and Structural Biology
 
 
 
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Contact

 

+44 (0)20 7594 5315s.j.matthews Website

 
 
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Location

 

602Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Grundy:2016:10.1038/ncomms12404,
author = {Grundy, GJ and Polo, LM and Zeng, Z and Rulten, SL and Hoch, NC and Paomephan, P and Xu, Y and Sweet, SM and Thorne, AW and Oliver, AW and Matthews, SJ and Pearl, LH and Caldecott, KW},
doi = {10.1038/ncomms12404},
journal = {Nature Communications},
title = {PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2).},
url = {http://dx.doi.org/10.1038/ncomms12404},
volume = {7},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - PARP3 is a member of the ADP-ribosyl transferase superfamily that we show accelerates the repair of chromosomal DNA single-strand breaks in avian DT40 cells. Two-dimensional nuclear magnetic resonance experiments reveal that PARP3 employs a conserved DNA-binding interface to detect and stably bind DNA breaks and to accumulate at sites of chromosome damage. PARP3 preferentially binds to and is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation activity to a single-histone substrate. Although nicks in naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation of histone H2B specifically at Glu2. These data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time, the ribosylation of chromatin at a site-specific DNA single-strand break.
AU  - Grundy,GJ
AU  - Polo,LM
AU  - Zeng,Z
AU  - Rulten,SL
AU  - Hoch,NC
AU  - Paomephan,P
AU  - Xu,Y
AU  - Sweet,SM
AU  - Thorne,AW
AU  - Oliver,AW
AU  - Matthews,SJ
AU  - Pearl,LH
AU  - Caldecott,KW
DO  - 10.1038/ncomms12404
PY  - 2016///
SN  - 2041-1723
TI  - PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2).
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms12404
UR  - http://hdl.handle.net/10044/1/39696
VL  - 7
ER  -
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