Imperial College London

ProfessorSebastianJohnston

Faculty of MedicineNational Heart & Lung Institute

Asthma UK Clinical Chair
 
 
 
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Contact

 

+44 (0)20 7594 3764s.johnston

 
 
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Assistant

 

Mr Christophe Tytgat +44 (0)20 7594 3849

 
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Location

 

343Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Publication Type
Year
to

527 results found

Hanratty CE, Matthews JG, Arron JR, Choy DF, Pavord ID, Bradding P, Brightling CE, Chaudhuri R, Cowan DC, Djukanovic R, Gallagher N, Fowler SJ, Hardman TC, Harrison T, Holweg CT, Howarth PH, Lordan J, Mansur AH, Menzies-Gow A, Mosesova S, Niven RM, Robinson DS, Shaw DE, Walker S, Woodcock A, Heaney LGet al., 2018, A randomised pragmatic trial of corticosteroid optimization in severe asthma using a composite biomarker algorithm to adjust corticosteroid dose versus standard care: study protocol for a randomised trial, TRIALS, Vol: 19, ISSN: 1745-6215

JOURNAL ARTICLE

Dhariwal J, Wong E, Trujillo-Torralbo B, Jackson DJ, Johnston SLet al., 2018, Poor Baseline Asthma Control Is Associated with Greater Virus Load Following Rhinovirus Infection, International Conference of the American-Thoracic-Society, Publisher: AMER THORACIC SOC, ISSN: 1073-449X

CONFERENCE PAPER

Ritchie AI, Singanayagam A, Wiater E, Edwards MR, Montminy M, Johnston SLet al., 2018, beta(2)-agonists enhance asthma-relevant inflammatory mediators in human airway epithelial cells, American Journal of Respiratory Cell and Molecular Biology, Vol: 58, Pages: 128-132, ISSN: 1044-1549

JOURNAL ARTICLE

Edwards MR, Walton RP, Jackson DJ, Feleszko W, Skevaki C, Jartti T, Makrinoti H, Nikonova A, Shilovskiy IP, Schwarze J, Johnston SL, Khaitov MRet al., 2018, The potential of anti-infectives and immunomodulators as therapies for asthma and asthma exacerbations, ALLERGY, Vol: 73, Pages: 50-63, ISSN: 0105-4538

JOURNAL ARTICLE

Tang HHF, Teo SM, Belgrave DCM, Evans M, Jackson D, Brozynska M, Kusel MMH, Johnston S, Gern J, Lemanske R, Simpson A, Custovic A, Sly P, Holt P, Holt K, Inouye Met al., 2017, Non-parametric mixture models identify trajectories of childhood immune development relevant to asthma and allergy

Events in early life contribute to subsequent risk of asthma; however, the causes and trajectories of childhood wheeze are heterogeneous and do not always result in asthma. Similarly, not all atopic individuals develop wheeze, and vice versa. The reasons for these differences are unclear. Using unsupervised model-based cluster analysis, we identified latent clusters within a prospective birth cohort with deep immunological and respiratory phenotyping. We characterised each cluster in terms of immunological profile and disease risk, and replicated our results in external cohorts from the UK and USA. We discovered three distinct trajectories, one of which is a high-risk "atopic" cluster with increased propensity for allergic diseases throughout childhood. Atopy contributes varyingly to later wheeze depending on cluster membership. Our findings demonstrate the utility of unsupervised analysis in elucidating heterogeneity in asthma pathogenesis and provide a foundation for improving management and prevention of childhood asthma.

WORKING PAPER

Finney LJ, Belchamber K, Kemp S, Donaldson G, Mallia P, Johnston SL, Wedzicha JAet al., 2017, HUMAN RHINOVIRUS IMPAIRS PHAGOCYTOSIS OF HAEMOPHILUS INFLUENZAE IN ALVEOLAR MACROPHAGES IN CHRONIC OBSTRUCTIVE PULMONARY DISEASE, Winter Meeting of the British-Thoracic-Society, Publisher: BMJ PUBLISHING GROUP, Pages: A112-A112, ISSN: 0040-6376

CONFERENCE PAPER

Teo SM, Tang HHF, Mok D, Judd L, Watts S, Pham K, Holt B, Kusel M, Serralha M, Troy N, Bochkov Y, Grindle K, Lemanske R, Johnston S, Gern J, Sly P, Holt P, Holt K, Inouye Met al., 2017, Dynamics of the upper airway microbiome in the pathogenesis of asthma-associated persistent wheeze in preschool children, Biorxiv

Repeated cycles of infection-associated lower airway inflammation drives the pathogenesis of persistent wheezing disease in children. Tracking these events across a birth cohort during their first five years, we demonstrate that >80% of infectious events indeed involve viral pathogens, but are accompanied by a shift in the nasopharyngeal microbiome (NPM) towards dominance by a small range of pathogenic bacterial genera. Unexpectedly, this change in NPM frequently precedes the appearance of viral pathogens and acute symptoms. In non-sensitized children these events are associated only with "transient wheeze" that resolves after age three. In contrast, in children developing early allergic sensitization, they are associated with ensuing development of persistent wheeze, which is the hallmark of the asthma phenotype. This suggests underlying pathogenic interactions between allergic sensitization and anti-bacterial mechanisms.

JOURNAL ARTICLE

Toussaint M, Jackson DJ, Swieboda D, Guedán A, Tsourouktsoglou T-D, Ching YM, Radermecker C, Makrinioti H, Aniscenko J, Edwards MR, Solari R, Farnir F, Papayannopoulos V, Bureau F, Marichal T, Johnston SLet al., 2017, Corrigendum: Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation., Nat Med, Vol: 23, Pages: 1384-1384

This corrects the article DOI: 10.1038/nm.4332.

JOURNAL ARTICLE

Vasileiou E, Sheikh A, Butler C, El Ferkh K, von Wissmann B, McMenamin J, Ritchie L, Schwarze J, Papadopoulos NG, Johnstone SL, Tian L, Simpson CRet al., 2017, Effectiveness of Influenza Vaccines in Asthma: A Systematic Review and Meta-Analysis, CLINICAL INFECTIOUS DISEASES, Vol: 65, Pages: 1388-1395, ISSN: 1058-4838

JOURNAL ARTICLE

Edwards MR, Strong K, Cameron A, Walton RP, Jackson DJ, Johnston SLet al., 2017, Viral infections in allergy and immunology: How allergic inflammation influences viral infections and illness, JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol: 140, Pages: 909-920, ISSN: 0091-6749

JOURNAL ARTICLE

Farne HA, Johnston SL, 2017, Immune mechanisms of respiratory viral infections in asthma, CURRENT OPINION IN IMMUNOLOGY, Vol: 48, Pages: 31-37, ISSN: 0952-7915

JOURNAL ARTICLE

Hellings PW, Fokkens WJ, Bachert C, Akdis CA, Bieber T, Agache I, Bernal-Sprekelsen M, Canonica GW, Gevaert P, Joos G, Lund V, Muraro A, Onerci M, Zuberbier T, Pugin B, Seys SF, Bousquet Jet al., 2017, Positioning the principles of precision medicine in care pathways for allergic rhinitis and chronic rhinosinusitis - A EUFOREA-ARIA-EPOS-AIRWAYS ICP statement, ALLERGY, Vol: 72, Pages: 1297-1305, ISSN: 0105-4538

JOURNAL ARTICLE

Upton N, Jackson DJ, Nikonova AA, Hingley-Wilson S, Khaitov M, del Rosario A, Traub S, Trujillo-Torralbo M-B, Habibi M, Elkin SL, Kon OM, Edwards MR, Mallia P, Footitt J, Macintyre J, Stanciu LA, Johnston SL, Sykes Aet al., 2017, Rhinovirus induction of fractalkine (CX3CL1) in airway and peripheral blood mononuclear cells in asthma, PLOS ONE, Vol: 12, ISSN: 1932-6203

JOURNAL ARTICLE

Haldar K, Bafadhel M, Lau K, Berg A, Kwambana B, Kebadze T, Ramsheh MY, Barker B, Haldar P, Johnston S, Ketley JM, Brightling CE, Barer MRet al., 2017, Microbiome balance in sputum determined by PCR stratifies COPD exacerbations and shows potential for selective use of antibiotics, PLOS ONE, Vol: 12, ISSN: 1932-6203

JOURNAL ARTICLE

Jha A, Jarvis H, Fraser C, Openshaw PJMet al., 2017, Respiratory Syncytial Virus

Respiratory syncytial virus (RSV) infection has an estimated global incidence of 33 million cases in children younger than 5 years, with 10% requiring hospital admission and up to 199,000 dying of the disease. There is growing evidence that severe infantile RSV bronchiolitis, a condition characterised by an inflammatory reaction to the virus, is associated with later childhood wheeze in some vulnerable children; however, a direct causal relationship with asthma has not yet been established. It is also increasingly recognised as a cause of morbidity and mortality in those with underlying airway disease, immunocompromise and frail elderly persons. Novel molecular based diagnostic tools are becoming established but treatment largely remains supportive, with palivizumab being the only licensed agent currently available for passive prophylaxis of selected pre-term infants. Whilst effective treatments remain elusive, there is optimism about the testing of novel antiviral drugs and the development of vaccines that may induce long-lasting immunity without the risk of disease augmentation.

BOOK CHAPTER

Dhariwal J, Cameron A, Trujillo-Torralbo M-B, del Rosario A, Bakhsoliani E, Paulsen M, Jackson DJ, Edwards MR, Rana BMJ, Cousins DJ, Hansel TT, Johnston SL, Walton RPet al., 2017, Mucosal Type 2 Innate Lymphoid Cells Are a Key Component of the Allergic Response to Aeroallergens, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 195, Pages: 1586-1596, ISSN: 1073-449X

JOURNAL ARTICLE

Petrova NV, Emelyanova AG, Gorbunov EA, Edwards MR, Walton RP, Bartlett NW, Aniscenko J, Gogsadze L, Bakhsoliani E, Khaitov MR, Johnston SL, Tarasov SA, Epstein OIet al., 2017, Efficacy of novel antibody-based drugs against rhinovirus infection: In vitro and in vivo results, ANTIVIRAL RESEARCH, Vol: 142, Pages: 185-192, ISSN: 0166-3542

JOURNAL ARTICLE

Toussaint M, Jackson DJ, Swieboda D, Guedan A, Tsourouktsoglou T-D, Ching YM, Radermecker C, Makrinioti H, Aniscenko J, Edwards MR, Solari R, Farnir F, Papayannopoulos V, Bureau F, Marichal T, Johnston SLet al., 2017, Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation, NATURE MEDICINE, Vol: 23, Pages: 681-+, ISSN: 1078-8956

JOURNAL ARTICLE

Prakash R, Hallett IC, Wong SF, Johnston SL, O'Donoghue EM, McAtee PA, Seal AG, Atkinson RG, Schroeder Ret al., 2017, Cell separation in kiwifruit without development of a specialised detachment zone, BMC PLANT BIOLOGY, Vol: 17, ISSN: 1471-2229

JOURNAL ARTICLE

Toussaint M, Swieboda D, Guedan A, Jackson DJ, Tsourouktsoglou D, Ching YM, Radermeker C, Makrinioti H, Aniescenko J, Edwards ME, Solari R, Farnir F, Papayannopoulos V, Bureau F, Marichal T, Johnston SLet al., NETosis and associated host DNA orchestrate rhinovirus-induced type 2 allergic asthma exacerbation, Nature Medicine, ISSN: 1546-170X

Respiratory viral infections represent the most common cause of allergic asthma exacerbations. Amplification of type 2 immunity is strongly implicated in asthma exacerbation, but how virus infection boosts type 2 responses during exacerbation is poorly understood. We report a significant correlation between release of host double stranded DNA (dsDNA) following rhinovirus infection and exacerbation of type 2 allergic inflammation and disease severity in patients. In a mouse model, we show that rhinovirus infection triggers neutrophil extracellular traps (NETs) formation and host dsDNA release. We further demonstrate that inhibiting NETosis by blocking neutrophil elastase or degrading NETs with DNase protects mice from type 2 allergic asthma exacerbations. Furthermore, injection of host dsDNA alone is sufficient to recapitulate many features of rhinovirus-induced type 2 immune responses and asthma pathology. Thus, NETosis and host dsDNA contribute to exacerbation pathogenesis and may represent potential targets for novel treatments of rhinovirus-induced asthma exacerbations.

JOURNAL ARTICLE

Papadopoulos NG, Androutsopoulou A, Akdis C, Dahlen S-E, Djukanovic R, Edwards J, Garcia-Marcos L, Johnston SL, Kupczyk M, Martin TR, Myles D, Palkonen S, Powell P, Riley J, Walker Set al., 2017, Asthma research in Europe: a transformative agenda for innovation and competitiveness, EUROPEAN RESPIRATORY JOURNAL, Vol: 49, ISSN: 0903-1936

JOURNAL ARTICLE

Hansel TT, Tunstall T, Trujillo-Torralbo M-B, Shamji B, del-Rosario A, Dhariwal J, Kirk PDW, Stumpf MPH, Koopmann J, Telcian A, Aniscenko J, Gogsadze L, Bakhsoliani E, Stanciu L, Bartlett N, Edwards M, Walton R, Mallia P, Hunt TM, Hunt TL, Hunt DG, Westwick J, Edwards M, Kon OM, Jackson DJ, Johnston SLet al., 2017, A Comprehensive Evaluation of Nasal and Bronchial Cytokines and Chemokines Following Experimental Rhinovirus Infection in Allergic Asthma: Increased Interferons (IFN-gamma and IFN-lambda) and Type 2 Inflammation (IL-5 and IL-13), EBIOMEDICINE, Vol: 19, Pages: 128-138, ISSN: 2352-3964

JOURNAL ARTICLE

Guedan A, Swieboda D, Charles M, Toussaint M, Johnston SL, Asfor A, Panjwani A, Tuthill TJ, Danahay H, Raynham T, Mousnier A, Solari Ret al., 2017, Investigation of the Role of Protein Kinase D in Human Rhinovirus Replication, JOURNAL OF VIROLOGY, Vol: 91, ISSN: 0022-538X

JOURNAL ARTICLE

Edwards MR, Saglani S, Schwarze J, Skevaki C, Smith JA, Ainsworth B, Almond M, Andreakos E, Belvisi MG, Chung KF, Cookson W, Cullinan P, Hawrylowicz C, Lommatzsch M, Jackson D, Lutter R, Marsland B, Moffatt M, Thomas M, Virchow JC, Xanthou G, Edwards J, Walker S, Johnston SLet al., 2017, Addressing unmet needs in understanding asthma mechanisms, EUROPEAN RESPIRATORY JOURNAL, Vol: 49, ISSN: 0903-1936

JOURNAL ARTICLE

Naveed S-U-N, Clements D, Jackson DJ, Philp C, Billington CK, Soomro I, Reynolds C, Harrison TW, Johnston SL, Shaw DE, Johnson SRet al., 2017, Matrix Metalloproteinase-1 Activation Contributes to Airway Smooth Muscle Growth and Asthma Severity, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 195, Pages: 1000-1009, ISSN: 1073-449X

JOURNAL ARTICLE

Johnston SL, Mansur A, Chauhan A, 2017, Incorrect Conclusions Concerning Antibiotics and Asthma Exacerbation, JAMA INTERNAL MEDICINE, Vol: 177, Pages: 598-598, ISSN: 2168-6106

JOURNAL ARTICLE

da Silva J, Hilzendeger C, Moermans C, Schleich F, Henket M, Kebadze T, Mallia P, Edwards MR, Johnston SL, Louis Ret al., 2017, Raised interferon-beta, type 3 interferon and interferon-stimulated genes - evidence of innate immune activation in neutrophilic asthma, CLINICAL AND EXPERIMENTAL ALLERGY, Vol: 47, Pages: 313-323, ISSN: 0954-7894

JOURNAL ARTICLE

Bousquet J, Farrell J, Crooks G, Hellings P, Bel EH, Bewick M, Chavannes NH, de Sousa JC, Cruz AA, Haahtela T, Joos G, Khaltaev N, Malva J, Muraro A, Nogues M, Palkonen S, Pedersen S, Robalo-Cordeiro C, Samolinski B, Strandberg T, Valiulis A, Yorgancioglu A, Zuberbier T, Bedbrook A, Aberer W, Adachi M, Agusti A, Akdis CA, Akdis M, Ankri J, Alonso A, Annesi-Maesano I, Ansotegui IJ, Anto JM, Arnavielhe S, Arshad H, Bai C, Baiardini I, Bachert C, Baigenzhin AK, Barbara C, Bateman ED, Beghe B, Ben Kheder A, Bennoor KS, Benson M, Bergmann KC, Bieber T, Bindslev-Jensen C, Bjermer L, Blain H, Blasi F, Boner AL, Bonini M, Bonini S, Bosnic-Anticevitch S, Boulet LP, Bourret R, Bousquet PJ, Braido F, Briggs AH, Brightling CE, Brozek J, Buhl R, Burney PG, Bush A, Caballero-Fonseca F, Caimmi D, Calderon MA, Calverley PM, Camargos PAM, Canonica GW, Camuzat T, Carlsen KH, Carr W, Carriazo A, Casale T, Sarabia AMC, Chatzi L, Chen YZ, Chiron R, Chkhartishvili E, Chuchalin AG, Chung KF, Ciprandi G, Cirule I, Cox L, Costa DJ, Custovic A, Dahl R, Dahlen SE, Darsow U, De Carlo G, De Blay F, Dedeu T, Deleanu D, Keenoy EDM, Demoly P, Denburg JA, Devillier P, Didier A, Dinh-Xuan AT, Djukanovic R, Dokic D, Douagui H, Dray G, Dubakiene R, Durham SR, Dykewicz MS, El-Gamal Y, Emuzyte R, Fabbri LM, Fletcher M, Fiocchi A, Wagner AF, Fonseca J, Fokkens WJ, Forastiere F, Frith P, Gaga M, Gamkrelidze A, Garces J, Garcia-Aymerich J, Gemicioglu B, Gereda JE, Diaz SG, Gotua M, Grisle I, Grouse L, Gutter Z, Guzman MA, Heaney LG, Hellquist-Dahl B, Henderson D, Hendry A, Heinrich J, Heve D, Horak F, Hourihane JOB, Howarth P, Humbert M, Hyland ME, Illario M, Ivancevich JC, Jardim JR, Jares EJ, Jeandel C, Jenkins C, Johnston SL, Jonquet O, Julge K, Jung KS, Just J, Kaidashev I, Khaitov MR, Kalayci O, Kalyoncu AF, Keil T, Keith PK, Klimek L, N'Goran BK, Kolek V, Koppelman GH, Kowalski ML, Kull I, Kuna P, Kvedariene V, Lambrecht B, Lau S, Larenas-Linnemann D, Laune D, Le LTT, Lieberman P, Lipworth B, Li J Cet al., 2017, Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing (Action Plan B3: Area 5) (vol 6, 29, 2016), CLINICAL AND TRANSLATIONAL ALLERGY, Vol: 7, ISSN: 2045-7022

JOURNAL ARTICLE

Aab A, Wirz O, van de Veen W, Sollner S, Stanic B, Ruckert B, Aniscenko J, Edwards MR, Johnston SL, Papadopoulos NG, Rebane A, Akdis CA, Akdis Met al., 2017, Human rhinoviruses enter and induce proliferation of B lymphocytes, ALLERGY, Vol: 72, Pages: 232-243, ISSN: 0105-4538

JOURNAL ARTICLE

Singanayagam A, Ritchie AI, Johnston SL, 2017, Role of microbiome in the pathophysiology and disease course of asthma, CURRENT OPINION IN PULMONARY MEDICINE, Vol: 23, Pages: 41-47, ISSN: 1070-5287

JOURNAL ARTICLE

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