Imperial College London

ProfessorSebastianJohnston

Faculty of MedicineNational Heart & Lung Institute

Asthma UK Clinical Chair
 
 
 
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Contact

 

+44 (0)20 7594 3764s.johnston

 
 
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Assistant

 

Mr Christophe Tytgat +44 (0)20 7594 3849

 
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Location

 

343Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Publication Type
Year
to

546 results found

Lan F, Zhang N, Wang DX, Krysko O, Holtappels G, Papadopoulos NG, Johnston SL, Bachert Cet al., 2014, Staphyloccoccus aureus elicit epithelial cell-derived cytokine productions in human nasal tissue, European-Academy-of-Allergy-and-Clinical-Immunology Congress, Publisher: WILEY-BLACKWELL, Pages: 169-169, ISSN: 0105-4538

Conference paper

Kupczyk M, Dahlen B, Sterk PJ, Nizankowska-Mogilnicka E, Papi A, Bel EH, Chanez P, Howarth PH, Holgate ST, Brusselle G, Siafakas NM, Gjomarkaj M, Dahlen S-Eet al., 2014, Stability of phenotypes defined by physiological variables and biomarkers in adults with asthma, ALLERGY, Vol: 69, Pages: 1198-1204, ISSN: 0105-4538

Journal article

Jayaraman A, Jackson DJ, Message SD, Pearson RM, Aniscenko J, Caramori G, Mallia P, Papi A, Shamji B, Edwards M, Westwick J, Hansel T, Stanciu LA, Johnston SL, Bartlett NWet al., 2014, IL-15 complexes induce NK- and T-cell responses independent of type I IFN signaling during rhinovirus infection, Publisher: NATURE PUBLISHING GROUP

Working paper

Yang ZB, Wan XL, Yang WR, Jiang SZ, Zhang GG, Johnston SL, Chi Fet al., 2014, Effects of naturally mycotoxin-contaminated corn on nutrient and energy utilization of ducks fed diets with or without Calibrin-A, POULTRY SCIENCE, Vol: 93, Pages: 2199-2209, ISSN: 0032-5791

Journal article

Bousquet J, Addis A, Adcock I, Agache I, Agusti A, Alonso A, Annesi-Maesano I, Anto JM, Bachert C, Baena-Cagnani CE, Bai C, Baigenzhin A, Barbara C, Barnes PJ, Bateman ED, Beck L, Bedbrook A, Bel EH, Benezet O, Bennoor KS, Benson M, Bernabeu-Wittel M, Bewick M, Bindslev-Jensen C, Blain H, Blasi F, Bonini M, Bonini S, Boulet LP, Bourdin A, Bourret R, Bousquet PJ, Brightling CE, Briggs A, Brozek J, Buhl R, Bush A, Caimmi D, Calderon M, Calverley P, Camargos PA, Camuzat T, Canonica GW, Carlsen KH, Casale TB, Cazzola M, Sarabia AMC, Cesario A, Chen YZ, Chkhartishvili E, Chavannes NH, Chiron R, Chuchalin A, Chung KF, Cox L, Crooks G, Crooks MG, Cruz AA, Custovic A, Dahl R, Dahlen SE, De Blay F, Dedeu T, Deleanu D, Demoly P, Devillier P, Didier A, Dinh-Xuan AT, Djukanovic R, Dokic D, Douagui H, Dubakiene R, Eglin S, Elliot F, Emuzyte R, Fabbri L, Wagner AF, Fletcher M, Fokkens WJ, Fonseca J, Franco A, Frith P, Furber A, Gaga M, Garces J, Garcia-Aymerich J, Gamkrelidze A, Gonzales-Diaz S, Gouzi F, Guzman MA, Haahtela T, Harrison D, Hayot M, Heaney LG, Heinrich J, Hellings PW, Hooper J, Humbert M, Hyland M, Iaccarino G, Jakovenko D, Jardim JR, Jeandel C, Jenkins C, Johnston SL, Jonquet O, Joos G, Jung KS, Kalayci O, Karunanithi S, Keil T, Khaltaev N, Kolek V, Kowalski ML, Kull I, Kuna P, Kvedariene V, Le LT, Carlsen KCL, Louis R, MacNee W, Mair A, Majer I, Manning P, Keenoy EDM, Masjedi MR, Meten E, Melo-Gomes E, Menzies-Gow A, Mercier G, Mercier J, Michel JP, Miculinic N, Mihaltan F, Milenkovic B, Molimard M, Momas I, Montilla-Santana A, Morais-Almeida M, Morgan M, N'Diaye M, Nafti S, Nekam K, Neou A, Nicod L, O'Hehir R, Ohta K, Paggiaro P, Palkonen S, Palmer S, Papadopoulos NG, Papi A, Passalacqua G, Pavord I, Pigearias B, Plavec D, Postma DS, Price D, Rabe KF, Pontal FR, Redon J, Rennard S, Roberts J, Robine JM, Roca J, Roche N, Rodenas F, Roggeri A, Rolland C, Rosado-Pinto J, Ryan D, Samolinski B, Sanchez-Borges M, Schunemann HJ, Sheikh A, Shields M, Siafakas N, Sibilleet al., 2014, Integrated care pathways for airway diseases (AIRWAYS-ICPs), EUROPEAN RESPIRATORY JOURNAL, Vol: 44, Pages: 304-323, ISSN: 0903-1936

Journal article

Wong EHC, Porter JD, Edwards MR, Johnston SLet al., 2014, The role of macrolides in asthma: current evidence and future directions, LANCET RESPIRATORY MEDICINE, Vol: 2, Pages: 657-670, ISSN: 2213-2600

Journal article

Ic-Jusufagic AS, Belgrave D, Pickles A, Telcian AG, Bakhsoliani E, Sykes A, Simpson A, Johnston SL, Custovic Aet al., 2014, Assessing the association of early life antibiotic prescription with asthma exacerbations, impaired antiviral immunity, and genetic variants in 17q21: a population-based birth cohort study, LANCET RESPIRATORY MEDICINE, Vol: 2, Pages: 621-630, ISSN: 2213-2600

Journal article

Djukanovic R, Harrison T, Johnston SL, Gabbay F, Wark P, Thomson NC, Niven R, Singh D, Reddel HK, Davies DE, Marsden R, Boxall C, Dudley S, Plagnol V, Holgate ST, Monk Pet al., 2014, The Effect of Inhaled IFN-beta on Worsening of Asthma Symptoms Caused by Viral Infections A Randomized Trial, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 190, Pages: 145-154, ISSN: 1073-449X

Journal article

Drysdale SB, Prendergast M, Alcazar M, Wilson T, Smith M, Zuckerman M, Broughton S, Rafferty GF, Johnston SL, Hodemaekers HM, Janssen R, Bont L, Greenough Aet al., 2014, Genetic predisposition of RSV infection-related respiratory morbidity in preterm infants, EUROPEAN JOURNAL OF PEDIATRICS, Vol: 173, Pages: 905-912, ISSN: 0340-6199

Journal article

Rohde G, Message SD, Haas JJ, Kebadze T, Parker H, Laza-Stanca V, Khaitov MR, Kon OM, Stanciu LA, Mallia P, Edwards MR, Johnston SLet al., 2014, CXC chemokines and antimicrobial peptides in rhinovirus-induced experimental asthma exacerbations, CLINICAL AND EXPERIMENTAL ALLERGY, Vol: 44, Pages: 930-939, ISSN: 0954-7894

Journal article

Drysdale SB, Alcazar M, Wilson T, Smith M, Zuckerman M, Lauinger IL, Tong CYW, Broughton S, Rafferty GF, Johnston SL, Greenough Aet al., 2014, Respiratory outcome of prematurely born infants following human rhinovirus A and C infections, EUROPEAN JOURNAL OF PEDIATRICS, Vol: 173, Pages: 913-919, ISSN: 0340-6199

Journal article

Khaitov MR, Shilovskiy IP, Nikonova AA, Shershakova NN, Kamyshnikov OY, Babakhin AA, Zverev VV, Johnston SL, Khaitov RMet al., 2014, Small Interfering RNAs Targeted to Interleukin-4 and Respiratory Syncytial Virus Reduce Airway Inflammation in a Mouse Model of Virus-Induced Asthma Exacerbation, HUMAN GENE THERAPY, Vol: 25, Pages: 642-650, ISSN: 1043-0342

Journal article

Hatchwell L, Girkin J, Dun MD, Morten M, Verrills N, Toop HD, Morris JC, Johnston SL, Foster PS, Collison A, Mattes Jet al., 2014, Salmeterol attenuates chemotactic responses in rhinovirus-induced exacerbation of allergic airways disease by modulating protein phosphatase 2A, JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol: 133, Pages: 1720-1727, ISSN: 0091-6749

Journal article

Suri R, Mallia P, Martin JE, Footitt J, Zhu J, Trujillo-Torralbo M-B, Johnston SL, Grigg Jet al., 2014, Bronchial platelet-activating factor receptor in chronic obstructive pulmonary disease, RESPIRATORY MEDICINE, Vol: 108, Pages: 898-904, ISSN: 0954-6111

Journal article

Zhu J, Message SD, Qiu Y, Mallia P, Kebadze T, Contoli M, Ward CK, Barnathan ES, Mascelli MA, Kon OM, Papi A, Stanciu LA, Jeffery PK, Johnston SLet al., 2014, Airway Inflammation and Illness Severity in Response to Experimental Rhinovirus Infection in Asthma, Chest, Vol: 145, Pages: 1219-1229, ISSN: 0012-3692

BackgroundThe nature of bronchial mucosal inflammation and its physiologic and clinical significance in rhinovirus-induced asthma exacerbations is unclear. We investigated bronchial mucosal inflammatory response and its association with physiologic and clinical outcomes in an experimental model of rhinovirus-induced asthma exacerbations.MethodsWe used immunohistochemistry methods to detect phenotypes of inflammatory cells infiltrating the bronchial mucosa before and after experimental rhinovirus infection in 10 subjects with asthma and 15 normal subjects.ResultsCompared with baseline, rhinovirus infection significantly increased the number of epithelial (P = .005) and subepithelial (P = .017) neutrophils in subjects with asthma only and subepithelial CD68+ macrophages in both subjects with asthma (P = .009) and normal subjects (P = .018) but more so in those with asthma (P = .021). Numbers of CD45+, CD68+, and CD20+ cells; neutrophils; and eosinophils at day 4 postinfection were positively associated with virus load (r = 0.50-0.72, P = .016-0.03). At acute infection in subjects with asthma, CD4+ cells correlated with chest symptom scores (r = 0.69, P = .029), the fall in the 10% fall in FEV1 (PC10) correlated with neutrophils (r = −0.89, P = .029), the PC10 correlated inversely with CD4+ (r = −0.67, P = .023) and CD8+ cells (r = −0.65, P = .03), the 20% fall in FEV1 was inversely associated with CD20+ cells (r = −0.65, P = .03), and higher epithelial CD8+ cell counts were significantly associated with a greater maximum fall in FEV1 (r = −0.72, P = .03), whereas higher subepithelial mast cell counts were significantly associated with a lower maximum percent fall in peak expiratory flow (r = 0.8, P = .024).ConclusionsIn subjects with asthma, rhinovirus infection induces bronchial mucosal neutrophilia and more severe monocyte/macrophage infiltration than in normal subjects. Airway neutrophils, eosinophils, and T and B lymphocytes during in

Journal article

Privolizzi R, Solari R, Johnston SL, McLean GRet al., 2014, The application of prophylactic antibodies for rhinovirus infections., Antivir Chem Chemother, Vol: 23, Pages: 173-177

Rhinoviruses are extremely common pathogens of the upper respiratory tract with adults experiencing on average 2-5 infections per year and children up to 12 infections. Although infections are not life threatening, except in cases of chronic lung disease where rhinoviruses are the major precipitant of acute exacerbations of disease, there is a high associated economic cost resulting from lost productivity due to absence from work or school. Treatment of infections focuses on symptom relief with anti-pyretics/analgesics as there are no antiviral therapies available and vaccine strategies face difficulties because of the large number of viral serotypes. Here, we assess the potential for prophylactic antibody intervention for these ubiquitous human pathogens.

Journal article

Adura PT, Reed E, Macintyre J, del Rosario A, Roberts J, Pestridge R, Beegan R, Boxall CB, Xiao C, Kebadze T, Aniscenko J, Cornelius V, Gern JE, Monk PD, Johnston SL, Djukanovic Ret al., 2014, Experimental rhinovirus 16 infection in moderate asthmatics on inhaled corticosteroids, EUROPEAN RESPIRATORY JOURNAL, Vol: 43, Pages: 1186-1189, ISSN: 0903-1936

Journal article

Reed DM, Foldes G, Gatheral T, Paschalaki KE, Lendvai Z, Bagyura Z, Nemeth T, Skopal J, Merkely B, Telcian AG, Gogsadze L, Edwards MR, Gough PJ, Bertin J, Johnston SL, Harding SE, Mitchell JAet al., 2014, Pathogen Sensing Pathways in Human Embryonic Stem Cell Derived-Endothelial Cells: Role of NOD1 Receptors, PLOS One, Vol: 9, ISSN: 1932-6203

Human embryonic stem cell-derived endothelial cells (hESC-EC), as well as other stem cell derived endothelial cells, have a range of applications in cardiovascular research and disease treatment. Endothelial cells sense Gram-negative bacteria via the pattern recognition receptors (PRR) Toll-like receptor (TLR)-4 and nucleotide-binding oligomerisation domain-containing protein (NOD)-1. These pathways are important in terms of sensing infection, but TLR4 is also associated with vascular inflammation and atherosclerosis. Here, we have compared TLR4 and NOD1 responses in hESC-EC with those of endothelial cells derived from other stem cells and with human umbilical vein endothelial cells (HUVEC). HUVEC, endothelial cells derived from blood progenitors (blood outgrowth endothelial cells; BOEC), and from induced pluripotent stem cells all displayed both a TLR4 and NOD1 response. However, hESC-EC had no TLR4 function, but did have functional NOD1 receptors. In vivo conditioning in nude rats did not confer TLR4 expression in hESC-EC. Despite having no TLR4 function, hESC-EC sensed Gram-negative bacteria, a response that was found to be mediated by NOD1 and the associated RIP2 signalling pathways. Thus, hESC-EC are TLR4 deficient but respond to bacteria via NOD1. This data suggests that hESC-EC may be protected from unwanted TLR4-mediated vascular inflammation, thus offering a potential therapeutic advantage.

Journal article

Mahmutovic-Persson I, Akbarshahi H, Bartlett NW, Glanville N, Johnston SL, Brandelius A, Uller Let al., 2014, Inhaled dsRNA and rhinovirus evoke neutrophilic exacerbation and lung expression of thymic stromal lymphopoietin in allergic mice with established experimental asthma, ALLERGY, Vol: 69, Pages: 348-358, ISSN: 0105-4538

Journal article

Sykes A, Macintyre J, Edwards MR, del Rosario A, Haas J, Gielen V, Kon OM, McHale M, Johnston SLet al., 2014, Rhinovirus-induced interferon production is not deficient in well controlled asthma, THORAX, Vol: 69, Pages: 240-246, ISSN: 0040-6376

Journal article

Makrinioti H, Toussaint M, Jackson DJ, Walton RP, Johnston SLet al., 2014, Role of interleukin 33 in respiratory allergy and asthma, LANCET RESPIRATORY MEDICINE, Vol: 2, Pages: 226-237, ISSN: 2213-2600

Journal article

Molyneaux P, Cox M, Willis-Owen S, Russell K, Mallia P, Russell A-M, Johnston S, Wells A, Cookson W, Maher T, Moffatt Met al., 2014, High Bacterial Load Predicts Poor Outcomes in Patients with Idiopathic Pulmonary Fibrosis

<jats:p>Background: Repetitive alveolar damage and aberrant repair may be important in the development of the fatal condition Idiopathic Pulmonary Fibrosis (IPF). The role played by microorganisms in this cycle is unknown. Methods: We consecutively enrolled patients diagnosed with IPF according to international criteria together with healthy smokers, non-smokers and subjects with moderate Chronic Obstructive Pulmonary Disease (COPD) as controls. Subjects underwent bronchoalveolar lavage (BAL) from which genomic DNA was isolated. The V3-V5 region of the bacterial 16S rRNA gene was amplified, allowing quantification of bacterial load and identification of communities by 16S rRNA qPCR and pyrosequencing. Results: Our 65 IPF patients had 3.9x109 copies of the 16S rRNA gene per ml of BAL, two-fold more than the 1.8x109 copies in 44 sex- and smoking-matched controls (P&lt;0.0001). Baseline BAL bacterial burden predicted Forced Vital Capacity (FVC) decline (P=0.02). Patients in the highest tertile of bacterial burden were at a higher risk of mortality compared to subjects in the lowest tertile (hazard ratio 4.59 (95% CI, 1.05-20); P=0.04). Sequencing yielded 912,883 high quality reads from all subjects. Operational Taxonomic Units (OTUs) representing Haemophilus, Streptococcus, Neisseria and Veillonella were 1.5 to 3.5 fold more abundant in cases than controls (P&lt;0.05). Regression analyses indicated that these specific OTUs as well as bacterial burden associated independently with IPF. Conclusions: IPF is characterised by an increased bacterial burden in BAL that predicts decline in lung function and death. Clinical trials of antimicrobial therapy may determine if microbial burden is causal or not in IPF progression.</jats:p>

Journal article

Majoor CJ, de Pol MAV, Kamphuisen PW, Meijers JCM, Molenkamp R, Wolthers KC, van der Poll T, Nieuwland R, Johnston SL, Sterk PJ, Bel EHD, Lutter R, van der Sluijs KFet al., 2014, Evaluation of coagulation activation after Rhinovirus infection in patients with asthma and healthy control subjects: an observational study, Respiratory Research, Vol: 15, ISSN: 1465-993X

BackgroundAsthma exacerbations are frequently triggered by rhinovirus infections. Both asthma and respiratory tract infection can activate haemostasis. Therefore we hypothesized that experimental rhinovirus-16 infection and asthmatic airway inflammation act in synergy on the haemostatic balance.Methods28 patients (14 patients with mild allergic asthma and 14 healthy non-allergic controls) were infected with low-dose rhinovirus type 16. Venous plasma and bronchoalveolar lavage fluid (BAL fluid) were obtained before and 6 days after infection to evaluate markers of coagulation activation, thrombin-antithrombin complexes, von Willebrand factor, plasmin-antiplasmin complexes, plasminogen activator inhibitor type-1, endogenous thrombin potential and tissue factor-exposing microparticles by fibrin generation test, in plasma and/or BAL fluid. Data were analysed by nonparametric tests (Wilcoxon, Mann Whitney and Spearman correlation).Results13 patients with mild asthma (6 females, 19-29 y) and 11 healthy controls (10 females, 19-31 y) had a documented Rhinovirus-16 infection. Rhinovirus-16 challenge resulted in a shortening of the fibrin generation test in BAL fluid of asthma patients (t = -1: 706 s vs. t = 6: 498 s; p = 0.02), but not of controls (t = -1: 693 s vs. t = 6: 636 s; p = 0.65). The fold change in tissue factor-exposing microparticles in BAL fluid inversely correlated with the fold changes in eosinophil cationic protein and myeloperoxidase in BAL fluid after virus infection (r = -0.517 and -0.528 resp., both p = 0.01).Rhinovirus-16 challenge led to increased plasminogen activator inhibitor type-1 levels in plasma in patients with asthma (26.0 ng/mL vs. 11.5 ng/mL in healthy controls, p = 0.04). Rhinovirus-16 load in BAL showed a linear correlation with the fold change in endogenous thrombin potential, plasmin-antiplasmin complexes and pla

Journal article

Jayaraman A, Bartlett N, Johnston SL, 2014, Innate and Adaptive Lymphocyte Responses In a Mouse Model Of Rhinovirus-Induced Asthma Exacerbation, Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI), Publisher: MOSBY-ELSEVIER, Pages: AB135-AB135, ISSN: 0091-6749

Conference paper

Kupczyk M, ten Brinke A, Sterk PJ, Bel EH, Papi A, Chanez P, Nizankowska-Mogilnicka E, Gjomarkaj M, Gaga M, Brusselle G, Dahlen B, Dahlen S-Eet al., 2014, Frequent exacerbators - a distinct phenotype of severe asthma, CLINICAL AND EXPERIMENTAL ALLERGY, Vol: 44, Pages: 212-221, ISSN: 0954-7894

Journal article

Gunawardana N, Finney L, Johnston SL, Mallia Pet al., 2014, Experimental rhinovirus infection in COPD: Implications for antiviral therapies, ANTIVIRAL RESEARCH, Vol: 102, Pages: 95-105, ISSN: 0166-3542

Journal article

Toussaint M, Singanayagam A, Johnston SL, Bartlett Net al., 2014, Role Of Interleukine-33 In Rhinovirus-Induced Allergic Asthma Exacerbation, Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI), Publisher: MOSBY-ELSEVIER, Pages: AB52-AB52, ISSN: 0091-6749

Conference paper

Mallia P, Message SD, Contoli M, Gray K, Telcian A, Laza-Stanca V, Papi A, Stanciu LA, Elkin S, Kon OM, Johnson M, Johnston SLet al., 2014, Lymphocyte subsets in experimental rhinovirus infection in chronic obstructive pulmonary disease, RESPIRATORY MEDICINE, Vol: 108, Pages: 78-85, ISSN: 0954-6111

Journal article

Majoor CJ, Van Der Pol MA, Kamphumen P, Meuers JCM, Van Der Poll T, Molenkamp R, Wolthers KC, Nieuwland R, Johnston SL, Sterk PJ, Bel E, Lulled R, Van Der Sluus KFet al., 2014, Hemostatic Activity Is Associated With Rhinovirus Load In Upper And Lower Airways In Asthma, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 189, ISSN: 1073-449X

Journal article

Jackson DJ, Shamji B, Trujillo-Torralbo M-B, Walton RP, Bartlett NW, Edwards MR, Mallia P, Edwards M, Westwick J, Johnston SLet al., 2014, Prostaglandin D2 Is Induced During Rhinovirus-Induced Asthma Exacerbations And Related To Exacerbation Severity In Vivo, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 189, ISSN: 1073-449X

Journal article

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