Imperial College London

ProfessorSebastianJohnston

Faculty of MedicineNational Heart & Lung Institute

Asthma UK Clinical Chair
 
 
 
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Contact

 

+44 (0)20 7594 3764s.johnston

 
 
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Assistant

 

Mr Christophe Tytgat +44 (0)20 7594 3849

 
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Location

 

343Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Farne:2018:10.3389/fcimb.2018.00432,
author = {Farne, H and Groves, H and Gill, S and stokes, I and Mccolloch, S and karoly, E and Trujillo-Torralbo, M and Johnston, S and Mallia, P and Tregoning, J},
doi = {10.3389/fcimb.2018.00432},
journal = {Frontiers in Cellular and Infection Microbiology},
title = {Comparative metabolomic sampling of upper and lower airways by four different methods to identify biochemicals that may support bacterial growth},
url = {http://dx.doi.org/10.3389/fcimb.2018.00432},
volume = {8},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Bacteria need nutrients from the host environment to survive, yet we know little about which biochemicals are present in the airways (the metabolome), which of these biochemicals are essential for bacterial growth and how they change with airway disease. The aims of this pilot study were to develop and compare methodologies for sampling the upper and lower airway metabolomes and to identify biochemicals present in the airways that could potentially support bacterial growth. Eight healthy human volunteers were sampled by four methods: two standard approaches - nasal lavage and induced sputum, and two using a novel platform, synthetic adsorptive matrix (SAM) strips—nasosorption and bronchosorption. Collected samples were analyzed by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectroscopy (UPLC-MS/MS). Five hundred and eighty-one biochemicals were recovered from the airways belonging to a range of metabolomic super-pathways. We observed significant differences between the sampling approaches. Significantly more biochemicals were recovered when SAM strips were used, compared to standard sampling techniques. A range of biochemicals that could support bacterial growth were detected in the different samples. This work demonstrates for the first time that SAM strips are a highly effective method for sampling the airway metabolome. This work will assist further studies to understand how changes in the airway metabolome affect bacterial infection in patients with underlying airway disease.
AU - Farne,H
AU - Groves,H
AU - Gill,S
AU - stokes,I
AU - Mccolloch,S
AU - karoly,E
AU - Trujillo-Torralbo,M
AU - Johnston,S
AU - Mallia,P
AU - Tregoning,J
DO - 10.3389/fcimb.2018.00432
PY - 2018///
SN - 2235-2988
TI - Comparative metabolomic sampling of upper and lower airways by four different methods to identify biochemicals that may support bacterial growth
T2 - Frontiers in Cellular and Infection Microbiology
UR - http://dx.doi.org/10.3389/fcimb.2018.00432
UR - http://hdl.handle.net/10044/1/66599
VL - 8
ER -