Imperial College London

ProfessorSebastianJohnston

Faculty of MedicineNational Heart & Lung Institute

Asthma UK Clinical Chair
 
 
 
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Contact

 

+44 (0)20 7594 3764s.johnston

 
 
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Assistant

 

Mr Christophe Tytgat +44 (0)20 7594 3849

 
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Location

 

343Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Greiller:2019:10.1016/j.jsbmb.2018.11.013,
author = {Greiller, CL and Suri, R and Jolliffe, DA and Kebadze, T and Hirsman, AG and Griffiths, CJ and Johnston, SL and Martineau, AR},
doi = {10.1016/j.jsbmb.2018.11.013},
journal = {Journal of Steroid Biochemistry and Molecular Biology},
pages = {152--159},
title = {Vitamin D attenuates rhinovirus-induced expression of intercellular adhesion molecule-1 (ICAM-1) and platelet-activating factor receptor (PAFR) in respiratory epithelial cells},
url = {http://dx.doi.org/10.1016/j.jsbmb.2018.11.013},
volume = {187},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Human rhinoviruses commonly cause upper respiratory infections, which may be complicated by secondary bacterial infection. Vitamin D replacement reduces risk of acute respiratory infections in vitamin D-deficient individuals, but the mechanisms by which such protection is mediated are incompletely understood. We therefore conducted experiments to characterise the influence of the major circulating metabolite 25-hydroxyvitamin D (25[OH]D) and the active metabolite 1,25-dihydroxyvitamin D (1,25[OH]2D) on responses of a respiratory epithelial cell line (A549 cells) to infection with a major group human rhinovirus (RV-16). Pre-treatment of A549 respiratory epithelial cells with a physiological concentration (10-7M) of 25(OH)D induced transient resistance to infection with RV-16 and attenuated RV-16-induced expression of the genes encoding intercellular adhesion molecule 1 (ICAM-1, a cell surface glycoprotein that acts as the cellular receptor for major group rhinoviruses) and platelet-activating factor receptor (PAFR, a G-protein coupled receptor implicated in adhesion of Streptococcus pneumoniae to respiratory epithelial cells). These effects were associated with enhanced expression of the genes encoding the NF-κB inhibitor IκBα and the antimicrobial peptide cathelicidin LL-37. Our findings suggest possible mechanisms by which vitamin D may enhance resistance to rhinovirus infection and reduce risk of secondary bacterial infection in vitamin D-deficient individuals.
AU - Greiller,CL
AU - Suri,R
AU - Jolliffe,DA
AU - Kebadze,T
AU - Hirsman,AG
AU - Griffiths,CJ
AU - Johnston,SL
AU - Martineau,AR
DO - 10.1016/j.jsbmb.2018.11.013
EP - 159
PY - 2019///
SN - 0960-0760
SP - 152
TI - Vitamin D attenuates rhinovirus-induced expression of intercellular adhesion molecule-1 (ICAM-1) and platelet-activating factor receptor (PAFR) in respiratory epithelial cells
T2 - Journal of Steroid Biochemistry and Molecular Biology
UR - http://dx.doi.org/10.1016/j.jsbmb.2018.11.013
UR - https://www.ncbi.nlm.nih.gov/pubmed/30476590
UR - http://hdl.handle.net/10044/1/66512
VL - 187
ER -