Imperial College London

ProfessorSebastianJohnston

Faculty of MedicineNational Heart & Lung Institute

Asthma UK Clinical Chair
 
 
 
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Contact

 

+44 (0)20 7594 3764s.johnston

 
 
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Assistant

 

Mr Christophe Tytgat +44 (0)20 7594 3849

 
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Location

 

343Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Toussaint:10.1038/nm.4332,
author = {Toussaint, M and Swieboda, D and Guedan, A and Jackson, DJ and Tsourouktsoglou, D and Ching, YM and Radermeker, C and Makrinioti, H and Aniescenko, J and Edwards, ME and Solari, R and Farnir, F and Papayannopoulos, V and Bureau, F and Marichal, T and Johnston, SL},
doi = {10.1038/nm.4332},
journal = {Nature Medicine},
title = {NETosis and associated host DNA orchestrate rhinovirus-induced type 2 allergic asthma exacerbation},
url = {http://dx.doi.org/10.1038/nm.4332},
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Respiratory viral infections represent the most common cause of allergic asthma exacerbations. Amplification of type 2 immunity is strongly implicated in asthma exacerbation, but how virus infection boosts type 2 responses during exacerbation is poorly understood. We report a significant correlation between release of host double stranded DNA (dsDNA) following rhinovirus infection and exacerbation of type 2 allergic inflammation and disease severity in patients. In a mouse model, we show that rhinovirus infection triggers neutrophil extracellular traps (NETs) formation and host dsDNA release. We further demonstrate that inhibiting NETosis by blocking neutrophil elastase or degrading NETs with DNase protects mice from type 2 allergic asthma exacerbations. Furthermore, injection of host dsDNA alone is sufficient to recapitulate many features of rhinovirus-induced type 2 immune responses and asthma pathology. Thus, NETosis and host dsDNA contribute to exacerbation pathogenesis and may represent potential targets for novel treatments of rhinovirus-induced asthma exacerbations.
AU - Toussaint,M
AU - Swieboda,D
AU - Guedan,A
AU - Jackson,DJ
AU - Tsourouktsoglou,D
AU - Ching,YM
AU - Radermeker,C
AU - Makrinioti,H
AU - Aniescenko,J
AU - Edwards,ME
AU - Solari,R
AU - Farnir,F
AU - Papayannopoulos,V
AU - Bureau,F
AU - Marichal,T
AU - Johnston,SL
DO - 10.1038/nm.4332
SN - 1546-170X
TI - NETosis and associated host DNA orchestrate rhinovirus-induced type 2 allergic asthma exacerbation
T2 - Nature Medicine
UR - http://dx.doi.org/10.1038/nm.4332
UR - http://hdl.handle.net/10044/1/44265
ER -