Imperial College London

ProfessorSebastianJohnston

Faculty of MedicineNational Heart & Lung Institute

Asthma UK Clinical Chair
 
 
 
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Contact

 

+44 (0)20 7594 3764s.johnston

 
 
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Assistant

 

Mr Christophe Tytgat +44 (0)20 7594 3849

 
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Location

 

343Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Toussaint:2017:10.1038/nm.4332,
author = {Toussaint, M and Jackson, DJ and Swieboda, D and Guedan, A and Tsourouktsoglou, T-D and Ching, YM and Radermecker, C and Makrinioti, H and Aniscenko, J and Edwards, MR and Solari, R and Farnir, F and Papayannopoulos, V and Bureau, F and Marichal, T and Johnston, SL},
doi = {10.1038/nm.4332},
journal = {NATURE MEDICINE},
pages = {681--691},
title = {Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation},
url = {http://dx.doi.org/10.1038/nm.4332},
volume = {23},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Respiratory viral infections represent the most common cause of allergic asthma exacerbations. Amplification of the type-2 immune response is strongly implicated in asthma exacerbation, but how virus infection boosts type-2 responses is poorly understood. We report a significant correlation between the release of host double-stranded DNA (dsDNA) following rhinovirus infection and the exacerbation of type-2 allergic inflammation in humans. In a mouse model of allergic airway hypersensitivity, we show that rhinovirus infection triggers dsDNA release associated with the formation of neutrophil extracellular traps (NETs), known as NETosis. We further demonstrate that inhibiting NETosis by blocking neutrophil elastase or by degrading NETs with DNase protects mice from type-2 immunopathology. Furthermore, the injection of mouse genomic DNA alone is sufficient to recapitulate many features of rhinovirus-induced type-2 immune responses and asthma pathology. Thus, NETosis and its associated extracellular dsDNA contribute to the pathogenesis and may represent potential therapeutic targets of rhinovirus-induced asthma exacerbations.
AU - Toussaint,M
AU - Jackson,DJ
AU - Swieboda,D
AU - Guedan,A
AU - Tsourouktsoglou,T-D
AU - Ching,YM
AU - Radermecker,C
AU - Makrinioti,H
AU - Aniscenko,J
AU - Edwards,MR
AU - Solari,R
AU - Farnir,F
AU - Papayannopoulos,V
AU - Bureau,F
AU - Marichal,T
AU - Johnston,SL
DO - 10.1038/nm.4332
EP - 691
PY - 2017///
SN - 1078-8956
SP - 681
TI - Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation
T2 - NATURE MEDICINE
UR - http://dx.doi.org/10.1038/nm.4332
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000402768000011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/49463
VL - 23
ER -