Imperial College London

ProfessorSebastianJohnston

Faculty of MedicineNational Heart & Lung Institute

Asthma UK Clinical Chair
 
 
 
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Contact

 

+44 (0)20 7594 3764s.johnston

 
 
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Assistant

 

Mr Christophe Tytgat +44 (0)20 7594 3849

 
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Location

 

343Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Moskwa:2018:10.4168/aair.2018.10.2.144,
author = {Moskwa, S and Piotrowski, W and Marczak, J and Pawelczyk, M and Lewandowska-Polak, A and Jarzebska, M and Brauncajs, M and Globinska, A and Gorski, P and Papadopoulos, NG and Edwards, MR and Johnston, SL and Kowalski, ML},
doi = {10.4168/aair.2018.10.2.144},
journal = {ALLERGY ASTHMA & IMMUNOLOGY RESEARCH},
pages = {144--154},
title = {Innate Immune Response to Viral Infections in Primary Bronchial Epithelial Cells is Modified by the Atopic Status of Asthmatic Patients},
url = {http://dx.doi.org/10.4168/aair.2018.10.2.144},
volume = {10},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - PurposeIn order to gain an insight into determinants of reported variability in immune responses to respiratory viruses in human bronchial epithelial cells (HBECs) from asthmatics, the responses of HBEC to viral infections were evaluated in HBECs from phenotypically heterogeneous groups of asthmatics and in healthy controls.MethodsHBECs were obtained during bronchoscopy from 10 patients with asthma (6 atopic and 4 non-atopic) and from healthy controls (n=9) and grown as undifferentiated cultures. HBECs were infected with parainfluenza virus (PIV)-3 (MOI 0.1) and rhinovirus (RV)-1B (MOI 0.1), or treated with medium alone. The cell supernatants were harvested at 8, 24, and 48 hours. IFN-α, CXCL10 (IP-10), and RANTES (CCL5) were analyzed by using Cytometric Bead Array (CBA), and interferon (IFN)-β and IFN-λ1 by ELISA. Gene expression of IFNs, chemokines, and IFN-regulatory factors (IRF-3 and IRF-7) was determined by using quantitative PCR.ResultsPIV3 and RV1B infections increased IFN-λ1 mRNA expression in HBECs from asthmatics and healthy controls to a similar extent, and virus-induced IFN-λ1 expression correlated positively with IRF-7 expression. Following PIV3 infection, IP-10 protein release and mRNA expression were significantly higher in asthmatics compared to healthy controls (median 36.03-fold). No differences in the release or expression of RANTES, IFN-λ1 protein and mRNA, or IFN-α and IFN-β mRNA between asthmatics and healthy controls were observed. However, when asthmatics were divided according to their atopic status, HBECs from atopic asthmatics (n=6) generated significantly more IFN-λ1 protein and demonstrated higher IFN-α, IFN-β, and IRF-7 mRNA expressions in response to PIV3 compared to non-atopic asthmatics (n=4) and healthy controls (n=9). In response to RV1B infection, IFN-β mRNA expression was lower (12.39-fold at 24 hours and 19.37-fold at 48 hours) in non-atopic asthmatics com
AU - Moskwa,S
AU - Piotrowski,W
AU - Marczak,J
AU - Pawelczyk,M
AU - Lewandowska-Polak,A
AU - Jarzebska,M
AU - Brauncajs,M
AU - Globinska,A
AU - Gorski,P
AU - Papadopoulos,NG
AU - Edwards,MR
AU - Johnston,SL
AU - Kowalski,ML
DO - 10.4168/aair.2018.10.2.144
EP - 154
PY - 2018///
SN - 2092-7355
SP - 144
TI - Innate Immune Response to Viral Infections in Primary Bronchial Epithelial Cells is Modified by the Atopic Status of Asthmatic Patients
T2 - ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
UR - http://dx.doi.org/10.4168/aair.2018.10.2.144
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000428003400007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/60328
VL - 10
ER -