Imperial College London
Alternate

ProfessorSebastianJohnston

Faculty of Medicine, National Heart & Lung Institute

Asthma UK Clinical Chair
 
 
 
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Contact

 

+44 (0)7931 376 544s.johnston

 
 
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Assistant

 

Mr Christophe Tytgat +44 (0)20 7594 3849

 
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Location

 

343Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dhariwal:2017:10.1164/rccm.201609-1846OC,
author = {Dhariwal, J and Cameron, A and Trujillo-Torralbo, MB and Del, Rosario A and Bakhsoliani, E and Paulsen, M and Jackson, DJ and Edwards, MR and Rana, BM and Cousins, DJ and Hansel, TT and Johnston, SL and Walton, RP and MRC-GSK, strategic alliance consortium},
doi = {10.1164/rccm.201609-1846OC},
journal = {American Journal of Respiratory and Critical Care Medicine},
pages = {1586--1596},
title = {Mucosal type 2 innate lymphoid cells are a key component of the allergic response to aeroallergen},
url = {http://dx.doi.org/10.1164/rccm.201609-1846OC},
volume = {195},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - RATIONALE: Newly characterised type 2 innate lymphoid cells display potent type 2 effector functionality, however their contribution to allergic airways inflammation and asthma is poorly understood. Mucosal biopsy used to characterise the airway mucosa is invasive, poorly tolerated and does not allow sequential sampling. OBJECTIVES: To assess the role of type 2 innate lymphoid cells during nasal allergen challenge in subjects with allergic rhinitis, using novel non-invasive methodology. METHODS: We used a human experimental allergen challenge model, with flow cytometric analysis of nasal curettage samples, to assess the recruitment of type 2 innate lymphoid cells and granulocytes to the upper airways of atopic and healthy subjects following allergen provocation. Soluble mediators in the nasal lining fluid were measured using nasosorption. MEASUREMENTS AND MAIN RESULTS: Following allergen challenge, atopic subjects displayed rapid induction of upper airway symptoms, an enrichment of type 2 innate lymphoid cells, eosinophils and neutrophils, along with increased production of interleukin-5, prostaglandin D2, and eosinophil and T-helper type 2 cell chemokines compared to healthy subjects. The most pronounced type 2 innate lymphoid cell recruitment was observed in patients with elevated serum IgE and airway eosinophilia. CONCLUSIONS: The rapid recruitment of type 2 innate lymphoid cells to the upper airways of allergic rhinitis patients, and their association with key type 2 mediators, highlights their likely important role in the early allergic response to aeroallergen in the airways. The novel methodology described herein enables the analysis of rare cell populations from non-invasive, serial tissue sampling.
AU  - Dhariwal,J
AU  - Cameron,A
AU  - Trujillo-Torralbo,MB
AU  - Del,Rosario A
AU  - Bakhsoliani,E
AU  - Paulsen,M
AU  - Jackson,DJ
AU  - Edwards,MR
AU  - Rana,BM
AU  - Cousins,DJ
AU  - Hansel,TT
AU  - Johnston,SL
AU  - Walton,RP
AU  - MRC-GSK,strategic alliance consortium
DO  - 10.1164/rccm.201609-1846OC
EP  - 1596
PY  - 2017///
SN  - 1535-4970
SP  - 1586
TI  - Mucosal type 2 innate lymphoid cells are a key component of the allergic response to aeroallergen
T2  - American Journal of Respiratory and Critical Care Medicine
UR  - http://dx.doi.org/10.1164/rccm.201609-1846OC
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28085492
UR  - https://www.atsjournals.org/doi/10.1164/rccm.201609-1846OC
UR  - http://hdl.handle.net/10044/1/44042
VL  - 195
ER  -
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