Imperial College London
Portrait Picture

ProfessorJ SimonKroll

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor,Paediatrics&Molecular Infectious Diseases
 
 
 
//

Contact

 

+44 (0)20 7594 3695s.kroll

 
 
//

Assistant

 

Dr Robert Boyle +44 (0)20 7594 3990

 
//

Location

 

245Wright Fleming WingSt Mary's Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Kiu:2019:10.3390/toxins11090543,
author = {Kiu, R and Sim, K and Shaw, A and Cornwell, E and Pickard, D and Kroll, JS and Hall, LJ},
doi = {10.3390/toxins11090543},
journal = {Toxins},
pages = {1--14},
title = {Genomic analysis of clostridium perfringens BEC/CPILE-positive, toxinotype D and E strains isolated from healthy children},
url = {http://dx.doi.org/10.3390/toxins11090543},
volume = {11},
year = {2019}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Clostridium perfringens toxinotype D, toxinotype E, and gastroenteritis-linked BEC/CPILE-positive strains have never been reported in healthy children. We isolated, whole-genome sequenced and bioinformatically characterised three C. perfringens isolates—type D (IQ1), type E (IQ2) and BEC/CPILE-positive (IQ3), recovered from the stools of three healthy two-year-olds, which were further compared to 128 C. perfringens genomes available from NCBI. The analysis uncovered a previously under-described putative toxin gene alv (alveolysin) encoded by isolates IQ2 and IQ3, which appeared to be a clade-specific trait associated with strains from domestic animals. A plasmid analysis indicated that the iota-toxin was encoded on a near-intact previously described plasmid pCPPB-1 in type E strain IQ2. The BEC genes becA and becB were carried on a near-identical pCPOS-1 plasmid previously associated with Japanese gastroenteritis outbreaks. Furthermore, a close phylogenetic relatedness was inferred between the French C. perfringens type E isolates cp515.17 and newly sequenced IQ2, suggesting geographical links. This study describes novel C. perfringens isolates from healthy individuals which encode important toxin genes, indicating the potential spread of these veterinary and clinically important strains and mobile genetic elements, and highlights areas for future research.
AU  - Kiu,R
AU  - Sim,K
AU  - Shaw,A
AU  - Cornwell,E
AU  - Pickard,D
AU  - Kroll,JS
AU  - Hall,LJ
DO  - 10.3390/toxins11090543
EP  - 14
PY  - 2019///
SN  - 2072-6651
SP  - 1
TI  - Genomic analysis of clostridium perfringens BEC/CPILE-positive, toxinotype D and E strains isolated from healthy children
T2  - Toxins
UR  - http://dx.doi.org/10.3390/toxins11090543
UR  - https://www.mdpi.com/2072-6651/11/9/543
UR  - http://hdl.handle.net/10044/1/73556
VL  - 11
ER  -
Download