Imperial College London
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ProfessorJ SimonKroll

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor,Paediatrics&Molecular Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3695s.kroll

 
 
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Assistant

 

Dr Robert Boyle +44 (0)20 7594 3990

 
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Location

 

245Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Shaw:2016:10.1097/MPG.0000000000001233,
author = {Shaw, AG and Black, N and Rushd, A and Sim, K and Randell, P and Kroll, JS and Epstein, J},
doi = {10.1097/MPG.0000000000001233},
journal = {Journal of Pediatric Gastroenterology and Nutrition},
pages = {230--237},
title = {Assessing the colonic microbiota in children:  effects of sample site and bowel preparation},
url = {http://dx.doi.org/10.1097/MPG.0000000000001233},
volume = {64},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ObjectivesInflammatory bowel disease (IBD) states are associated with gastrointestinal dysbiosis. Mucosal biopsy sampling, retrieving the bacterial community that most directly interacts with the host, is an invasive procedure, and we hypothesize may be sufficiently approximated by other sampling methods. We investigate the relatedness of samples obtained by different methods and the effects of bowel preparation on the gastrointestinal community in a paediatric population. MethodsWe recruited a cohort of patients undergoing colonoscopy, collecting serial samples via differing methods (rectal swabs, biopsies and faecal matter/luminal contents) pre-bowel preparation, during colonoscopy and post-colonoscopy. Next generation sequencing was used to determine the structure of the microbial community. ResultsThe microbial community in luminal contents collected during colonoscopy was found to be more similar to that of mucosal biopsies than rectal swabs. Community traits of the mucosal biopsies could be used to segregate IBD patients from other patients, and the similarity of the communities in the luminal contents was sufficient for the segregation to be reproduced. Microbial communities sampled by rectal swabs and pre-bowel preparation faeces were less similar to mucosal biopsies. Bowel preparation was found to have no significant long term effects on the microbial community, despite the transient effects evident during colonoscopy. ConclusionsA clinically relevant description of the mucosal microbial community can be obtained via the non-invasive collection of luminal contents after bowel cleansing. Bowel preparation in a paediatric population results in no consistent sustained alterations to the gastrointestinal microbiota.
AU  - Shaw,AG
AU  - Black,N
AU  - Rushd,A
AU  - Sim,K
AU  - Randell,P
AU  - Kroll,JS
AU  - Epstein,J
DO  - 10.1097/MPG.0000000000001233
EP  - 237
PY  - 2016///
SN  - 1536-4801
SP  - 230
TI  - Assessing the colonic microbiota in children:  effects of sample site and bowel preparation
T2  - Journal of Pediatric Gastroenterology and Nutrition
UR  - http://dx.doi.org/10.1097/MPG.0000000000001233
UR  - http://hdl.handle.net/10044/1/30856
VL  - 64
ER  -
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