Imperial College London
Portrait Picture

ProfessorJ SimonKroll

Faculty of Medicine, Department of Infectious Disease

Emeritus Professor,Paediatrics&Molecular Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3695s.kroll

 
 
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Assistant

 

Dr Robert Boyle +44 (0)20 7594 3990

 
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Location

 

245Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rose:2017:10.7717/peerj.2928,
author = {Rose, G and Shaw, AG and Sim, K and Wooldridge, DJ and Li, M-S and Gharbia, S and Misra, RV and Kroll, J},
doi = {10.7717/peerj.2928},
journal = {PeerJ},
title = {Antibiotic resistance potential of the healthy preterm infant gut microbiome},
url = {http://dx.doi.org/10.7717/peerj.2928},
volume = {5},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: Few studies have investigated the gut microbiome of infants, fewer still preterm infants. In this study we sought to quantify and interrogate the resistome within a cohort of premature infants using shotgun metagenomic sequencing. We describe the gut microbiomes from preterm but healthy infants, characterising the taxonomic diversity identified and frequency of antibiotic resistance genes detected.Results: Dominant clinically important species identified within the microbiomes included C. perfringens, K. pneumoniae and members of the Staphylococci and Enterobacter genera. Screening at the gene level we identified an average 13 genes per preterm infant, ranging across 8 different antibiotic classes, including aminoglycosides and fluoroquinolones. Some antibiotic resistance genes were associated with clinically relevant bacteria, including the identification of mecA and high levels of Staphylococci within some infants. We were able to demonstrate that in a third of the infants the S. aureus identified was unrelated using MLST or metagenome assembly, but low abundance prevented such analysis within the remaining samples.Conclusions: We found that the healthy preterm infant gut microbiomes in this study harboured a significant diversity of antibiotic resistance genes. This broad picture of resistances and the wider taxonomic diversity identified raises further caution to the use of antibiotics without consideration of the resident microbial communities.
AU  - Rose,G
AU  - Shaw,AG
AU  - Sim,K
AU  - Wooldridge,DJ
AU  - Li,M-S
AU  - Gharbia,S
AU  - Misra,RV
AU  - Kroll,J
DO  - 10.7717/peerj.2928
PY  - 2017///
SN  - 2167-8359
TI  - Antibiotic resistance potential of the healthy preterm infant gut microbiome
T2  - PeerJ
UR  - http://dx.doi.org/10.7717/peerj.2928
UR  - http://hdl.handle.net/10044/1/43565
VL  - 5
ER  -
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