Imperial College London

ProfessorSteveMarston

Faculty of MedicineNational Heart & Lung Institute

(Non-Clinical) Professor in Cardiovascular Biochemistry
 
 
 
//

Contact

 

+44 (0)20 7594 2732s.marston Website

 
 
//

Location

 

433ICTEM buildingHammersmith Campus

//

Summary

 

Publications

Publication Type
Year
to

188 results found

Gusev NB, Bukach OV, Marston SB, 2005, Structure, properties, and probable physiological role of small heat shock protein with molecular mass 20 kD (Hsp20, HspB6), BIOCHEMISTRY-MOSCOW, Vol: 70, Pages: 629-637, ISSN: 0006-2979

Journal article

Holohan S-JP, Marston SB, 2005, Force-velocity relationship of single actin filament interacting with immobilised myosin measured by electromagnetic technique., IEE Proc Nanobiotechnol, Vol: 152, Pages: 113-120, ISSN: 1478-1581

The effect of applying an external load to actin filaments moving in the in vitro motility assay is studied. Bead-tailed actin filaments were made by polymerising actin onto 2.8 microm diameter Dynabeads conjugated with gelsolin-G actin. These were introduced into a motility cell coated with 100 microg/ml rabbit fast skeletal myosin in the presence of ATP and 0.5% methylcellulose. The motility cell was inserted between the pole-pieces of an electromagnet and the fluorescent beads and filaments were observed. The force-current relationship of the electromagnet was determined from the velocity of free beads in viscous solution and Stokes' equation. The magnet produced up to 6 pN force on the Dynabeads at 1 A. Many bead-tailed actin filaments stuck to the surface, but the beads that did move moved at the same speed as unloaded f-actin in the same cell. Bead-tailed filaments slowed down under an increasing magnetic load, eventually stalled and then slid backward under increasing load before detaching from the surface. Single-filament force-velocity curves were constructed and a stalling force of about 0.6 pN/mm of actin filament estimated.

Journal article

Bukach OV, Marston SB, Gusev NB, 2005, Small heat shock protein with apparent molecular mass 20 kDa (Hsp20, HspB6) is not a genuine actin-binding protein, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 26, Pages: 175-181, ISSN: 0142-4319

Journal article

Chernik IS, Panasenko OO, Li Y, Marston SB, Gusev NBet al., 2004, pH-induced changes of the structure of small heat shock proteins with molecular mass 24/27 kDa (HspB1), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 324, Pages: 1199-1203, ISSN: 0006-291X

Journal article

Kim MV, Seit-Nebi AS, Marston SB, Gusev NBet al., 2004, Some properties of human small heat shock protein Hsp22 (H11 or HspB8), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 315, Pages: 796-801, ISSN: 0006-291X

Journal article

Marston S, Mirza M, Abdulrazzak H, Sewry Cet al., 2004, Functional characterisation of a mutant actin (Met132Val) from a patient with nemaline myopathy, NEUROMUSCULAR DISORDERS, Vol: 14, Pages: 167-174, ISSN: 0960-8966

Journal article

Bukach OV, Seit-Nebi AS, Marston SB, Gusev NBet al., 2004, Some properties of human small heat shock protein Hsp20 (HspB6), EUROPEAN JOURNAL OF BIOCHEMISTRY, Vol: 271, Pages: 291-302, ISSN: 0014-2956

Journal article

Marston SB, Redwood CS, 2003, Modulation of thin filament activation by breakdown or isoform switching of thin filament proteins - Physiological and pathological implications, CIRCULATION RESEARCH, Vol: 93, Pages: 1170-1178, ISSN: 0009-7330

Journal article

Panasenko OO, Kim MV, Marston SB, Gusev NBet al., 2003, Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin, EUROPEAN JOURNAL OF BIOCHEMISTRY, Vol: 270, Pages: 892-901, ISSN: 0014-2956

Journal article

Ansari S, El-Mezgueldi M, Marston S, 2003, Cooperative inhibition of actin filaments in the absence of tropomyosin, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 24, Pages: 513-520, ISSN: 0142-4319

Journal article

Marston S, 2003, Random walks with thin filaments: application of in vitro motility assay to the study of actomyosin regulation, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 24, Pages: 149-156, ISSN: 0142-4319

Journal article

Panasenko OO, Seit-Nebi A, Bukach OV, Marston SB, Gusev NBet al., 2002, Structure and properties of avian small heat shock protein with molecular weight 25 kDa, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1601, Pages: 64-74, ISSN: 1570-9639

Journal article

Robinson P, Mirza M, Knott A, Abdulrazzak H, Willott R, Marston S, Watkins H, Redwood Cet al., 2002, Alterations in thin filament regulation induced by a human cardiac troponin T mutant that causes dilated cardiomyopathy are distinct from those induced by troponin T mutants that cause hypertrophic cardiomyopathy, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 277, Pages: 40710-40716, ISSN: 0021-9258

Journal article

Marston SB, Ingwall JS, Glueck SB, 2002, Calcium, contractions, and tropomyosin Focus on "Divergent abnormal muscle relaxation by hypertrophic cardiomyopathy and nemaline myopathy mutant tropomyosins", PHYSIOLOGICAL GENOMICS, Vol: 9, Pages: 57-58, ISSN: 1094-8341

Journal article

Gusev NB, Bogatcheva NV, Marston SB, 2002, Structure and properties of small heat shock proteins (sHsp) and their interaction with cytoskeleton proteins, BIOCHEMISTRY-MOSCOW, Vol: 67, Pages: 511-519, ISSN: 0006-2979

Journal article

Patchell VB, Vorotnikov AV, Gao Y, Low DG, Evans JS, Fattoum A, El-Mezgueldi M, Marston SB, Levine BAet al., 2002, Phosphorylation of the minimal inhibitory region at the C-terminus of caldesmon alters its structural and actin binding properties, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, Vol: 1596, Pages: 121-130, ISSN: 0167-4838

Journal article

Knott A, Purcell I, Marston S, 2002, In vitro motility analysis of thin filaments from failing and non-failing human heart: Troponin from failing human hearts induces slower filament sliding and higher Ca2+ sensitivity, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 34, Pages: 469-482, ISSN: 0022-2828

Journal article

Burton D, Abdulrazzak H, Knott A, Elliott K, Redwood C, Watkins H, Marston S, Ashley Cet al., 2002, Two mutations in troponin I that cause hypertrophic cardiomyopathy have contrasting effects on cardiac muscle contractility, BIOCHEMICAL JOURNAL, Vol: 362, Pages: 443-451, ISSN: 0264-6021

Journal article

Gusev NB, Bogatcheva NV, Marston S, 2002, Structure and properties of small heat-shock proteins (sHsp) and their interactions with cytoskeletal proteins, Biokhimiia, Vol: 67, Pages: 613-623, ISSN: 0320-9725

Journal article

Borovikov YS, Avrova SV, Vikhoreva NN, Vikhorev PG, Ermakov VS, Copeland O, Marston SBet al., 2001, C-terminal actin-binding sites of smooth muscle caldesmon switch actin between conformational states, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol: 33, Pages: 1151-1159, ISSN: 1357-2725

Journal article

Goncharova EA, Shirinsky VP, Shevelev AY, Marston SB, Vorotnikov AVet al., 2001, Actomyosin cross-linking by caldesmon in non-muscle cells, FEBS LETTERS, Vol: 497, Pages: 113-117, ISSN: 0014-5793

Journal article

Medvedeva MV, Djemuchadze DR, Watterson DM, Marston SB, Gusev NBet al., 2001, Replacement of Lys-75 of calmodulin affects its interaction with smooth muscle caldesmon, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, Vol: 1544, Pages: 143-150, ISSN: 0167-4838

Journal article

Marston SB, Hodgkinson JL, 2001, Cardiac and skeletal myopathies: can genotype explain phenotype?, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 22, Pages: 1-4, ISSN: 0142-4319

Journal article

El Mezgueldi M, Marston SB, 2001, Caldesmon, Pages: 428-430, ISBN: 9780471374947

Book chapter

Bing W, Knott A, Redwood C, Esposito G, Purcell I, Watkins H, Marston Set al., 2000, Effect of hypertrophic cardiomyopathy mutations in human cardiac muscle alpha-tropomyosin (Asp175Asn and Glu180Gly) on the regulatory properties of human cardiac troponin determined by in vitro motility assay, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 32, Pages: 1489-1498, ISSN: 0022-2828

Journal article

Notarianni G, Gusev N, Lafitte D, Hill TJ, Cooper HS, Derrick PJ, Marston SBet al., 2000, A novel Ca2+ binding protein associated with caldesmon in Ca2+-regulated smooth muscle thin filaments: evidence for a structurally altered form of calmodulin, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 21, Pages: 537-549, ISSN: 0142-4319

Journal article

Redwood C, Lohmann K, Bing W, Esposito GM, Elliott K, Abdulrazzak H, Knott A, Purcell I, Marston S, Watkins Het al., 2000, Investigation of a truncated cardiac troponin T that causes familial hypertrophic cardiomyopathy - Ca2+ regulatory properties of reconstituted thin filaments depend on the ratio of mutant to wild-type protein, CIRCULATION RESEARCH, Vol: 86, Pages: 1146-1152, ISSN: 0009-7330

Journal article

Vikhorev PG, Avrova SV, Ermakov VS, Copeland O, Marston SB, Borovikov ISet al., 2000, [Effect of the C-terminal actin-binding sites of caldesmon on the interaction of actin with myosin]., Tsitologiia, Vol: 42, Pages: 1069-1074, ISSN: 0041-3771

TRITC-phalloidin or FITC-labeled F-actin of ghost muscle fibers was bound to tropomyosin and C-terminal recombinant fragments of caldesmon CaDH1 (residues 506-793) or CaDH2 (residues 683-767). After that the fibers were decorated with myosin subfragment 1. In the absence of caldesmon fragments, subfragment 1 interaction with F-actin caused changes in parameters of polarized fluorescence, that were typical of "strong" binding of myosin heads to F-actin and of the "switched on" conformational state of actin. CaDH1 inhibited, whereas CaDH2 activated the effect of subfragment 1. It is suggested that C-terminal part of caldesmon may modulate the transition of F-actin subunits from the "switched on" to the "switched off" state.

Journal article

Gao Y, Patchell VB, Huber PAJ, Copeland O, El-Mezgueldi M, Fattoum A, Calas B, Thorsted PB, Marston SB, Levine BAet al., 1999, The interface between caldesmon domain 4b and subdomain 1 of actin studied by nuclear magnetic resonance spectroscopy, BIOCHEMISTRY, Vol: 38, Pages: 15459-15469, ISSN: 0006-2960

Journal article

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.

Request URL: http://wlsprd.imperial.ac.uk:80/respub/WEB-INF/jsp/search-html.jsp Request URI: /respub/WEB-INF/jsp/search-html.jsp Query String: limit=30&id=00155869&person=true&page=4&respub-action=search.html