Publications
198 results found
Jacques A, Copeland O, Messer A, et al., 2008, Myosin binding protein C phosphorylation in normal, hypertrophic and failing human heart muscle., J Mol Cell Cardiol, Vol: 45, Pages: 209-216
Phosphorylation of myosin binding protein C (MyBP-C) was investigated in intraventricular septum samples taken from patients with hypertrophic cardiomyopathy undergoing surgical septal myectomy. These samples were compared with donor heart muscle, as a well-characterised control tissue, and with end-stage failing heart muscle. MyBP-C was partly purified from myofibrils using a modification of the phosphate-EDTA extraction of Hartzell and Glass. MyBP-C was separated by SDS-PAGE and stained for phosphoproteins using Pro-Q Diamond followed by total protein staining using Coomassie Blue. Relative phosphorylation level was determined from the ratio of Pro-Q Diamond to Coomassie Blue staining of MyBP-C bands as measured by densitometry. We compared 9 myectomy samples and 9 failing heart samples with 9 donor samples. MyBP-C phosphorylation in pathological muscle was lower than in donor (myectomy 40+/-2% of donor, P<0.0001; failing 45+/-3% of donor, P<0.0001). 6 myectomy samples were identified with MYBPC3 mutations, one with MYH7 mutation and two remained unknown, but there was no correlation between MYBPC3 mutation and MyBP-C phosphorylation level. In order to determine the number of phosphorylated sites in human cardiac MyBP-C samples, we phosphorylated the recombinant MyBP-C fragment, C0-C2 (1-453) with PKA using (gamma32)P-ATP up to 3.5 mol Pi/mol C0-C2. This measurement of phosphorylation was used to calibrate measurements of phosphorylation in SDS-PAGE using Pro-Q Diamond stain. The level of phosphorylation in donor heart MyBP-C was calculated to be 4.6+/-0.6 mol Pi/mol and 2.0+/-0.3 mol Pi/mol in myectomy samples. We conclude that MyBP-C is a highly phosphorylated protein in vivo and that diminished MyBP-C phosphorylation is a feature of both end-stage heart failure and hypertrophic cardiomyopathy.
Kasakov AS, Bukach OV, Seit-Nebi AS, et al., 2007, Effect of mutations in the β5-β7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11), FEBS JOURNAL, Vol: 274, Pages: 5628-5642, ISSN: 1742-464X
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- Citations: 35
Clarke NF, Ilkovski B, Cooper S, et al., 2007, The pathogenesis of <i>ACTA1</i>-related congenital fiber type disproportion, ANNALS OF NEUROLOGY, Vol: 61, Pages: 552-561, ISSN: 0364-5134
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- Citations: 45
Mirza M, Robinson P, Kremneva E, et al., 2007, The effect of mutations in α-tropomyosin (E40K and E54K) that cause familial dilated cardiomyopathy on the regulatory mechanism of cardiac muscle thin filaments, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 282, Pages: 13487-13497, ISSN: 0021-9258
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- Citations: 64
Chernik IS, Seit-Nebi AS, Marston SB, et al., 2007, Small heat shock protein Hsp20 (HspB6) as a partner of 14-3-3γ, MOLECULAR AND CELLULAR BIOCHEMISTRY, Vol: 295, Pages: 9-17, ISSN: 0300-8177
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- Citations: 64
Messer AE, Marston SB, Jacques AM, 2007, Troponin phosphorylation and regulatory function in human heart muscle: dephosphorylation of Ser23/24 on troponin I could account for the contractile defect in end-stage heart failure, Journal of Molecular and Cellular Cardiology, Vol: 42, Pages: 247-259
Kim MV, Kasakov AS, Seit-Nebi AS, et al., 2006, Structure and properties of K141E mutant of small heat shock protein HSP22 (HspB8, H11) that is expressed in human neuromuscular disorders, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, Vol: 454, Pages: 32-41, ISSN: 0003-9861
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- Citations: 39
D'Amico A, Graziano C, Pacileo G, et al., 2006, Fatal hypertrophic cardiomyopathy and nemaline myopathy associated with ACTA1 K336E mutation, NEUROMUSCULAR DISORDERS, Vol: 16, Pages: 548-552, ISSN: 0960-8966
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- Citations: 60
Alahyan M, Webb MR, Marston SB, et al., 2006, The mechanism of smooth muscle caldesmon-tropomyosin inhibition of the elementary steps of the actomyosin ATPase, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 19433-19448
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- Citations: 17
Mirza M, Marston S, Willott R, et al., 2005, Dilated cardiomyopathy mutations in three thin filament regulatory proteins result in a common functional phenotype, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 280, Pages: 28498-28506
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- Citations: 128
Holohan S-JP, Marston SB, 2005, Force-velocity relationship of single actin filament interacting with immobilised myosin measured by electromagnetic technique., IEE Proc Nanobiotechnol, Vol: 152, Pages: 113-120, ISSN: 1478-1581
The effect of applying an external load to actin filaments moving in the in vitro motility assay is studied. Bead-tailed actin filaments were made by polymerising actin onto 2.8 microm diameter Dynabeads conjugated with gelsolin-G actin. These were introduced into a motility cell coated with 100 microg/ml rabbit fast skeletal myosin in the presence of ATP and 0.5% methylcellulose. The motility cell was inserted between the pole-pieces of an electromagnet and the fluorescent beads and filaments were observed. The force-current relationship of the electromagnet was determined from the velocity of free beads in viscous solution and Stokes' equation. The magnet produced up to 6 pN force on the Dynabeads at 1 A. Many bead-tailed actin filaments stuck to the surface, but the beads that did move moved at the same speed as unloaded f-actin in the same cell. Bead-tailed filaments slowed down under an increasing magnetic load, eventually stalled and then slid backward under increasing load before detaching from the surface. Single-filament force-velocity curves were constructed and a stalling force of about 0.6 pN/mm of actin filament estimated.
Gusev NB, Bukach OV, Marston SB, 2005, Structure, properties, and probable physiological role of small heat shock protein with molecular mass 20 kD (Hsp20, HspB6), BIOCHEMISTRY-MOSCOW, Vol: 70, Pages: 629-637, ISSN: 0006-2979
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- Citations: 32
Bukach OV, Marston SB, Gusev NB, 2005, Small heat shock protein with apparent molecular mass 20 kDa (Hsp20, HspB6) is not a genuine actin-binding protein, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 26, Pages: 175-181, ISSN: 0142-4319
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- Citations: 14
Chernik IS, Panasenko OO, Li Y, et al., 2004, pH-induced changes of the structure of small heat shock proteins with molecular mass 24/27 kDa (HspB1), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 324, Pages: 1199-1203, ISSN: 0006-291X
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- Citations: 23
Kim MV, Seit-Nebi AS, Marston SB, et al., 2004, Some properties of human small heat shock protein Hsp22 (H11 or HspB8), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 315, Pages: 796-801, ISSN: 0006-291X
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- Citations: 54
Marston S, Mirza M, Abdulrazzak H, et al., 2004, Functional characterisation of a mutant actin (Met132Val) from a patient with nemaline myopathy, NEUROMUSCULAR DISORDERS, Vol: 14, Pages: 167-174, ISSN: 0960-8966
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- Citations: 21
Bukach OV, Seit-Nebi AS, Marston SB, et al., 2004, Some properties of human small heat shock protein Hsp20 (HspB6), EUROPEAN JOURNAL OF BIOCHEMISTRY, Vol: 271, Pages: 291-302, ISSN: 0014-2956
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- Citations: 100
Marston SB, Redwood CS, 2003, Modulation of thin filament activation by breakdown or isoform switching of thin filament proteins - Physiological and pathological implications, CIRCULATION RESEARCH, Vol: 93, Pages: 1170-1178, ISSN: 0009-7330
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- Citations: 63
Panasenko OO, Kim MV, Marston SB, et al., 2003, Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin, EUROPEAN JOURNAL OF BIOCHEMISTRY, Vol: 270, Pages: 892-901, ISSN: 0014-2956
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- Citations: 38
Ansari S, El-Mezgueldi M, Marston S, 2003, Cooperative inhibition of actin filaments in the absence of tropomyosin, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 24, Pages: 513-520, ISSN: 0142-4319
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- Citations: 7
Marston S, 2003, Random walks with thin filaments:: application of <i>in vitro</i> motility assay to the study of actomyosin regulation, JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, Vol: 24, Pages: 149-156, ISSN: 0142-4319
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- Citations: 16
Panasenko OO, Seit-Nebi A, Bukach OV, et al., 2002, Structure and properties of avian small heat shock protein with molecular weight 25 kDa, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Vol: 1601, Pages: 64-74, ISSN: 1570-9639
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- Citations: 18
Robinson P, Mirza M, Knott A, et al., 2002, Alterations in thin filament regulation induced by a human cardiac troponin T mutant that causes dilated cardiomyopathy are distinct from those induced by troponin T mutants that cause hypertrophic cardiomyopathy, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 277, Pages: 40710-40716, ISSN: 0021-9258
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- Citations: 108
Marston SB, Ingwall JS, Glueck SB, 2002, <i>Calcium, contractions, and tropomyosin</i> Focus on "Divergent abnormal muscle relaxation by hypertrophic cardiomyopathy and nemaline myopathy mutant tropomyosins", PHYSIOLOGICAL GENOMICS, Vol: 9, Pages: 57-58, ISSN: 1094-8341
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- Citations: 4
Gusev NB, Bogatcheva NV, Marston SB, 2002, Structure and properties of small heat shock proteins (sHsp) and their interaction with cytoskeleton proteins, BIOCHEMISTRY-MOSCOW, Vol: 67, Pages: 511-519, ISSN: 0006-2979
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- Citations: 139
Patchell VB, Vorotnikov AV, Gao Y, et al., 2002, Phosphorylation of the minimal inhibitory region at the C-terminus of caldesmon alters its structural and actin binding properties, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, Vol: 1596, Pages: 121-130, ISSN: 0167-4838
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- Citations: 15
Knott A, Purcell I, Marston S, 2002, <i>In vitro</i> motility analysis of thin filaments from failing and non-failing human heart:: Troponin from failing human hearts induces slower filament sliding and higher Ca<SUP>2+</SUP> sensitivity, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 34, Pages: 469-482, ISSN: 0022-2828
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- Citations: 33
Burton D, Abdulrazzak H, Knott A, et al., 2002, Two mutations in troponin I that cause hypertrophic cardiomyopathy have contrasting effects on cardiac muscle contractility, BIOCHEMICAL JOURNAL, Vol: 362, Pages: 443-451, ISSN: 0264-6021
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- Citations: 30
Gusev NB, Bogatcheva NV, Marston S, 2002, Structure and properties of small heat-shock proteins (sHsp) and their interactions with cytoskeletal proteins, Biokhimiia, Vol: 67, Pages: 613-623, ISSN: 0320-9725
Borovikov YS, Avrova SV, Vikhoreva NN, et al., 2001, C-terminal actin-binding sites of smooth muscle caldesmon switch actin between conformational states, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol: 33, Pages: 1151-1159, ISSN: 1357-2725
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- Citations: 10
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