Imperial College London
Portrait Picture

ProfessorSteveMarston

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor
 
 
 
//

Contact

 

+44 (0)20 7594 2732s.marston Website

 
 
//

Location

 

433ICTEM buildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Dos:2017:10.1007/s12551-017-0305-3,
author = {Dos, Remedios CG and Lal, SP and Li, A and McNamara, J and Keogh, A and Macdonald, PS and Cooke, R and Ehler, E and Knöll, R and Marston, SB and Stelzer, J and Granzier, H and Bezzina, C and van, Dijk S and De, Man F and Stienen, GJM and Odeberg, J and Pontén, F and Linke, WA and Linke, W and van, der Velden J},
doi = {10.1007/s12551-017-0305-3},
journal = {Biophys Rev},
pages = {431--441},
title = {The Sydney Heart Bank: improving translational research while eliminating or reducing the use of animal models of human heart disease.},
url = {http://dx.doi.org/10.1007/s12551-017-0305-3},
volume = {9},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The Sydney Heart Bank (SHB) is one of the largest human heart tissue banks in existence. Its mission is to provide high-quality human heart tissue for research into the molecular basis of human heart failure by working collaboratively with experts in this field. We argue that, by comparing tissues from failing human hearts with age-matched non-failing healthy donor hearts, the results will be more relevant than research using animal models, particularly if their physiology is very different from humans. Tissue from heart surgery must generally be used soon after collection or it significantly deteriorates. Freezing is an option but it raises concerns that freezing causes substantial damage at the cellular and molecular level. The SHB contains failing samples from heart transplant patients and others who provided informed consent for the use of their tissue for research. All samples are cryopreserved in liquid nitrogen within 40 min of their removal from the patient, and in less than 5-10 min in the case of coronary arteries and left ventricle samples. To date, the SHB has collected tissue from about 450 failing hearts (>15,000 samples) from patients with a wide range of etiologies as well as increasing numbers of cardiomyectomy samples from patients with hypertrophic cardiomyopathy. The Bank also has hearts from over 120 healthy organ donors whose hearts, for a variety of reasons (mainly tissue-type incompatibility with waiting heart transplant recipients), could not be used for transplantation. Donor hearts were collected by the St Vincent's Hospital Heart and Lung transplantation team from local hospitals or within a 4-h jet flight from Sydney. They were flushed with chilled cardioplegic solution and transported to Sydney where they were quickly cryopreserved in small samples. Failing and/or donor samples have been used by more than 60 research teams around the world, and have resulted in more than 100 research papers. The tissues most commonly reques
AU  - Dos,Remedios CG
AU  - Lal,SP
AU  - Li,A
AU  - McNamara,J
AU  - Keogh,A
AU  - Macdonald,PS
AU  - Cooke,R
AU  - Ehler,E
AU  - Knöll,R
AU  - Marston,SB
AU  - Stelzer,J
AU  - Granzier,H
AU  - Bezzina,C
AU  - van,Dijk S
AU  - De,Man F
AU  - Stienen,GJM
AU  - Odeberg,J
AU  - Pontén,F
AU  - Linke,WA
AU  - Linke,W
AU  - van,der Velden J
DO  - 10.1007/s12551-017-0305-3
EP  - 441
PY  - 2017///
SN  - 1867-2450
SP  - 431
TI  - The Sydney Heart Bank: improving translational research while eliminating or reducing the use of animal models of human heart disease.
T2  - Biophys Rev
UR  - http://dx.doi.org/10.1007/s12551-017-0305-3
UR  - https://www.ncbi.nlm.nih.gov/pubmed/28808947
VL  - 9
ER  -
Download