78 results found
Lee PP, Lobato-Marquez D, Pramanik N, et al., 2017, Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723
Liew N, Moya MJM, Wierzbicki CJ, et al., 2017, Chytrid fungus infection in zebrafish demonstrates that the pathogen can parasitize non-amphibian vertebrate hosts, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723
Lobato-Marquez D, Mostowy S, 2017, Salmonella ubiquitination: ARIH1 enters the fray, EMBO REPORTS, Vol: 18, Pages: 1476-1477, ISSN: 1469-221X
Mazon-Moya MJ, Willis AR, Torraca V, et al., 2017, Septins restrict inflammation and protect zebrafish larvae from Shigella infection, PLOS PATHOGENS, Vol: 13, ISSN: 1553-7366
McCarthy RR, Mazon-Moya MJ, Moscoso JA, et al., 2017, Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion, NATURE MICROBIOLOGY, Vol: 2, ISSN: 2058-5276
Mesquita FS, Brito C, Mazon Moya MJ, et al., 2017, Endoplasmic reticulum chaperone Gp96 controls actomyosin dynamics and protects against pore-forming toxins, EMBO REPORTS, Vol: 18, Pages: 303-318, ISSN: 1469-221X
Ribet D, Boscaini S, Cauvin C, et al., 2017, SUMOylation of human septins is critical for septin filament bundling and cytokinesis., J Cell Biol, Vol: 216, Pages: 4041-4052
Septins are cytoskeletal proteins that assemble into nonpolar filaments. They are critical in diverse cellular functions, acting as scaffolds for protein recruitment and as diffusion barriers for subcellular compartmentalization. Human septins are encoded by 13 different genes and are classified into four groups based on sequence homology (SEPT2, SEPT3, SEPT6, and SEPT7 groups). In yeast, septins were among the first proteins reported to be modified by SUMOylation, a ubiquitin-like posttranslational modification. However, whether human septins could be modified by small ubiquitin-like modifiers (SUMOs) and what roles this modification may have in septin function remains unknown. In this study, we first show that septins from all four human septin groups can be covalently modified by SUMOs. We show in particular that endogenous SEPT7 is constitutively SUMOylated during the cell cycle. We then map SUMOylation sites to the C-terminal domain of septins belonging to the SEPT6 and SEPT7 groups and to the N-terminal domain of septins from the SEPT3 group. We finally demonstrate that expression of non-SUMOylatable septin variants from the SEPT6 and SEPT7 groups leads to aberrant septin bundle formation and defects in cytokinesis after furrow ingression. Altogether, our results demonstrate a pivotal role for SUMOylation in septin filament bundling and cell division.
Torraca V, Mostowy S, 2017, Zebrafish Infection: From Pathogenesis to Cell Biology., Trends Cell Biol
The study of host-pathogen interactions has illuminated fundamental research avenues in both infection and cell biology. Zebrafish (Danio rerio) larvae are genetically tractable, optically accessible, and present a fully functional innate immune system with macrophages and neutrophils that mimic their mammalian counterparts. A wide variety of pathogenic bacteria have been investigated using zebrafish models, providing unprecedented resolution of the cellular response to infection in vivo. In this review, we illustrate how zebrafish models have contributed to our understanding of cellular microbiology by providing an in vivo platform to study host-pathogen interactions from the single cell to whole animal level. We also highlight discoveries made from zebrafish infection that hold great promise for translation into novel therapies for humans.
Klionsky DJ, Abdelmohsen K, Abe A, et al., 2016, Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition), AUTOPHAGY, Vol: 12, Pages: 1-222, ISSN: 1554-8627
Krokowski S, Lobato-Márquez D, Mostowy S, 2016, Mitochondria promote septin assembly into cages that entrap Shigella for autophagy., Autophagy
Septins are cytoskeletal proteins implicated in cytokinesis and host-pathogen interactions. During macroautophagy/autophagy of Shigella flexneri, septins assemble into cage-like structures to entrap actin-polymerizing bacteria and restrict their dissemination. How septins assemble to entrap bacteria is not fully known. We discovered that mitochondria support septin cage assembly to promote autophagy of Shigella. Consistent with roles for the cytoskeleton in mitochondrial dynamics, we showed that DNM1L/DRP1 (dynamin 1 like) can interact with septins to enhance mitochondrial fission. Remarkably, Shigella fragment mitochondria and escape from septin cage entrapment in order to avoid autophagy. These results uncover a close relationship between mitochondria and septin assembly, and identify a new role for mitochondria in bacterial autophagy.
Krokowski S, Mostowy S, 2016, Interactions between Shigella flexneri and the Autophagy Machinery, FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, Vol: 6, ISSN: 2235-2988
Krokowski S, Mostowy S, 2016, Investigation of septins using infection by bacterial pathogens, SEPTINS, Vol: 136, Pages: 117-134, ISSN: 0091-679X
Larrouy-Maumus G, Clements A, Filloux A, et al., 2016, Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry, JOURNAL OF MICROBIOLOGICAL METHODS, Vol: 120, Pages: 68-71, ISSN: 0167-7012
Lobato-Marquez D, Mostowy S, 2016, Septins recognize micron-scale membrane curvature, JOURNAL OF CELL BIOLOGY, Vol: 213, Pages: 5-6, ISSN: 0021-9525
Pfanzelter J, Way M, Mostowy S, 2016, Septins suppress the release of vaccinia virus from infected cells., Annual Meeting of the American-Society-for-Cell-Biology (ASCB), Publisher: AMER SOC CELL BIOLOGY, ISSN: 1059-1524
Shah A, Kannambath S, Herbst S, et al., 2016, Calcineurin Orchestrates Lateral Transfer of Aspergillus fumigatus during Macrophage Cell Death, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 194, Pages: 1127-1139, ISSN: 1073-449X
Sirianni A, Krokowski S, Lobato-Marquez D, et al., 2016, Mitochondria mediate septin cage assembly to promote autophagy of Shigella, EMBO REPORTS, Vol: 17, Pages: 1029-1043, ISSN: 1469-221X
Torraca V, Mostowy S, 2016, Septins and Bacterial Infection., Front Cell Dev Biol, Vol: 4, ISSN: 2296-634X
Septins, a unique cytoskeletal component associated with cellular membranes, are increasingly recognized as having important roles in host defense against bacterial infection. A role for septins during invasion of Listeria monocytogenes into host cells was first proposed in 2002. Since then, work has shown that septins assemble in response to a wide variety of invasive bacterial pathogens, and septin assemblies can have different roles during the bacterial infection process. Here we review the interplay between septins and bacterial pathogens, highlighting septins as a structural determinant of host defense. We also discuss how investigation of septin assembly in response to bacterial infection can yield insight into basic cellular processes including phagocytosis, autophagy, and mitochondrial dynamics.
Willis A, Mazon-Moya M, Mostowy S, 2016, Investigation of septin biology in vivo using zebrafish, SEPTINS, Vol: 136, Pages: 221-241, ISSN: 0091-679X
Willis AR, Moore C, Mazon-Moya M, et al., 2016, Injections of Predatory Bacteria Work Alongside Host Immune Cells to Treat Shigella Infection in Zebrafish Larvae, CURRENT BIOLOGY, Vol: 26, Pages: 3343-3351, ISSN: 0960-9822
Gibbings D, Mostowy S, Jay F, et al., 2015, Corrigendum: Selective autophagy degrades DICER and AGO2 and regulates miRNA activity., Nat Cell Biol, Vol: 17
Gibbings D, Mostowy S, Jay F, et al., 2015, Selective autophagy degrades DICER and AGO2 and regulates miRNA activity (vol 14, pg 1314, 2012), NATURE CELL BIOLOGY, Vol: 17, Pages: 1088-1088, ISSN: 1465-7392
Herbst S, Shah A, Moya MM, et al., 2015, Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus, EMBO MOLECULAR MEDICINE, Vol: 7, Pages: 240-258, ISSN: 1757-4676
Mostowy S, Shenoy AR, 2015, The cytoskeleton in cell-autonomous immunity: structural determinants of host defence, NATURE REVIEWS IMMUNOLOGY, Vol: 15, Pages: 559-573, ISSN: 1474-1733
Shah A, Kannambath S, Herbst S, et al., 2015, 'THE KISS OF DEATH' - CALCINEURIN INHIBITORS PREVENT ACTIN-DEPENDENT LATERAL TRANSFER OF ASPERGILLUS FUMIGATUS IN NECROPTOTIC HUMAN MACROPHAGES, Winter Meeting of the British-Thoracic-Society, Publisher: BMJ PUBLISHING GROUP, Pages: A48-A48, ISSN: 0040-6376
Sirianni A, Mostowy S, 2015, Autophagy in the Infected Cell: Insights from Pathogenic Bacteria, Autophagy, Infection, and the Immune Response, Pages: 143-157, ISBN: 9781118677551
© 2015 by John Wiley & Sons, Inc. All rights reserved. Autophagy is an important defence mechanism to clear intracellular microbes. A wide variety of bacterial pathogens are targeted to autophagy; however, some have evolved mechanisms to avoid or manipulate the autophagy machinery for intracellular survival. An in-depth understanding of autophagy-bacteria interactions will therefore be critical to appreciate fully how the autophagy machinery can function in immunity and microbial clearance in vivo.
Mostowy S, 2014, Multiple Roles of the Cytoskeleton in Bacterial Autophagy, PLOS PATHOGENS, Vol: 10, ISSN: 1553-7366
Mostowy S, Bi E, Fuchtbauer E-M, et al., 2014, Highlight: The 5th International Workshop on Septin Biology, BIOLOGICAL CHEMISTRY, Vol: 395, Pages: 119-121, ISSN: 1431-6730
Moya MJM, Colucci-Guyon E, Mostowy S, 2014, Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
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