Imperial College London
Alternate

ProfessorStephenSmith

Faculty of Engineering, Department of Civil and Environmental Engineering

Professor of Bioresource Systems
 
 
 
//

Contact

 

+44 (0)20 7594 6051s.r.smith

 
 
//

Assistant

 

Miss Judith Barritt +44 (0)20 7594 5967

 
//

Location

 

229Skempton BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Karkashan:2015:10.1016/j.watres.2014.10.003,
author = {Karkashan, A and Khallaf, B and Morris, J and Thurbon, N and Rouch, D and Smith, SR and Deighton, M},
doi = {10.1016/j.watres.2014.10.003},
journal = {Water Research},
pages = {533--544},
title = {Comparison of methodologies for enumerating and detecting the viability of Ascaris eggs in sewage sludge by standard incubation-microscopy, the BacLight Live/Dead viability assay and other vital dyes},
url = {http://dx.doi.org/10.1016/j.watres.2014.10.003},
volume = {68},
year = {2015}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The aim of this study was to evaluate the Live/Dead BacLight viability kit as a method for enumerating viable eggs of Ascaris suum in sewage sludge as a surrogate for the human roundworm. The number and viability status of eggs of A. suum were accurately measured directly in sewage sludge samples by the BacLight method, compared to the conventional incubation-microscopy procedure. BacLight stains were not toxic to A. suum eggs, in contrast to some conventional vital dyes which disrupted viable eggs. The method was effective for the direct examination of eggs in heavily contaminated samples or seeded sludge containing ∼200 eggs/g DS in sludge with 5% DS content. However, a recovery method would be necessary to examine samples with small numbers of eggs, for instance in sludge from regions where the prevalence of infection with Ascaris lumbricoides is low. The BacLight technique may therefore be an effective alternative to conventional incubation-microscopy for enumerating Ascaris eggs in contaminated field samples or to validate sludge treatment processes by examining decay rates of inoculated A. suum eggs in laboratory simulations. Most field samples would require recovery from an appropriate number of composite samples prior to vital staining.
AU  - Karkashan,A
AU  - Khallaf,B
AU  - Morris,J
AU  - Thurbon,N
AU  - Rouch,D
AU  - Smith,SR
AU  - Deighton,M
DO  - 10.1016/j.watres.2014.10.003
EP  - 544
PY  - 2015///
SN  - 0043-1354
SP  - 533
TI  - Comparison of methodologies for enumerating and detecting the viability of Ascaris eggs in sewage sludge by standard incubation-microscopy, the BacLight Live/Dead viability assay and other vital dyes
T2  - Water Research
UR  - http://dx.doi.org/10.1016/j.watres.2014.10.003
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000347756900048&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S0043135414006988
VL  - 68
ER  -
Download