Imperial College London

ProfessorSivarameshWigneshweraraj

Faculty of MedicineDepartment of Medicine

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 1867s.r.wig

 
 
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Location

 

4.40BFlowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sarkar:2017:nar/gkx733,
author = {Sarkar, P and Switzer, A and Peters, C and Pogliano, J and Wigneshweraraj, S},
doi = {nar/gkx733},
journal = {Nucleic Acids Res},
title = {Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.},
url = {http://dx.doi.org/10.1093/nar/gkx733},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Many bacterial adaptive responses to changes in growth conditions due to biotic and abiotic factors involve reprogramming of gene expression at the transcription level. The bacterial RNA polymerase (RNAP), which catalyzes transcription, can thus be considered as the major mediator of cellular adaptive strategies. But how do bacteria respond if a stress factor directly compromises the activity of the RNAP? We used a phage-derived small protein to specifically perturb bacterial RNAP activity in exponentially growing Escherichia coli. Using cytological profiling, tracking RNAP behavior at single-molecule level and transcriptome analysis, we reveal that adaptation to conditions that directly perturb bacterial RNAP performance can result in a biphasic growth behavior and thereby confer the 'adapted' bacterial cells an enhanced ability to tolerate diverse antibacterial stresses. The results imply that while synthetic transcriptional rewiring may confer bacteria with the intended desirable properties, such approaches may also collaterally allow them to acquire undesirable traits.
AU - Sarkar,P
AU - Switzer,A
AU - Peters,C
AU - Pogliano,J
AU - Wigneshweraraj,S
DO - nar/gkx733
PY - 2017///
TI - Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.
T2 - Nucleic Acids Res
UR - http://dx.doi.org/10.1093/nar/gkx733
UR - https://www.ncbi.nlm.nih.gov/pubmed/28977482
UR - http://hdl.handle.net/10044/1/50382
ER -