Imperial College London
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Professor Ramesh Wigneshweraraj

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 1867s.r.wig

 
 
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Location

 

4.40BFlowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tabib-Salazar:2018:10.1073/pnas.1800429115,
author = {Tabib-Salazar, A and Liu, B and Declan, B and Burchell, L and Qimron, U and Matthews, S and Wigneshweraraj, S},
doi = {10.1073/pnas.1800429115},
journal = {Proceedings of the National Academy of Sciences},
pages = {E5353--E5362},
title = {T7 phage factor required for managing RpoS in Escherichia coli},
url = {http://dx.doi.org/10.1073/pnas.1800429115},
volume = {115},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - T7 development in Escherichia coli requires the inhibition of the housekeepingform of the bacterial RNA polymerase (RNAP), Eσ70, by two T7 proteins: Gp2and Gp5.7. While the biological role of Gp2 is well understood, that of Gp5.7remains to be fully deciphered. Here, we present results from functional andstructural analyses to reveal that Gp5.7 primarily serves to inhibit EσS, thepredominant form of the RNAP in the stationary phase of growth, whichaccumulates in exponentially growing E. coli as a consequence of buildup ofguanosine pentaphosphate ((p)ppGpp) during T7 development. We furtherdemonstrate a requirement of Gp5.7 for T7 development in E. coli cells in thestationary phase of growth. Our finding represents a paradigm for how somelytic phages have evolved distinct mechanisms to inhibit the bacterialtranscription machinery to facilitate phage development in bacteria in theexponential and stationary phases of growth.
AU  - Tabib-Salazar,A
AU  - Liu,B
AU  - Declan,B
AU  - Burchell,L
AU  - Qimron,U
AU  - Matthews,S
AU  - Wigneshweraraj,S
DO  - 10.1073/pnas.1800429115
EP  - 5362
PY  - 2018///
SN  - 0027-8424
SP  - 5353
TI  - T7 phage factor required for managing RpoS in Escherichia coli
T2  - Proceedings of the National Academy of Sciences
UR  - http://dx.doi.org/10.1073/pnas.1800429115
UR  - https://www.pnas.org/content/115/23/E5353/tab-article-info
UR  - http://hdl.handle.net/10044/1/59116
VL  - 115
ER  -
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