Imperial College London
Portrait Picture

Professor Ramesh Wigneshweraraj

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
//

Contact

 

+44 (0)20 7594 1867s.r.wig

 
 
//

Location

 

4.40BFlowers buildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Switzer:2018:10.1099/mic.0.000683,
author = {Switzer, A and Evangelopoulos, D and Figueira, R and Carvalho, L and Brown, D and Wigneshweraraj, S},
doi = {10.1099/mic.0.000683},
journal = {Microbiology},
pages = {1457--1470},
title = {A novel regulatory factor affecting the transcription of methionine biosynthesis genes in Escherichia coli experiencing sustained nitrogen starvation},
url = {http://dx.doi.org/10.1099/mic.0.000683},
volume = {164},
year = {2018}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The initial adaptive transcriptional response to nitrogen (N) starvation in Escherichia coli involves large-scale alterations to the transcriptome mediated by the transcriptional activator, NtrC. One of these NtrC-activated genes is yeaG, which encodes a conserved bacterial kinase. Although it is known that YeaG is required for optimal survival under sustained N starvation, the molecular basis by which YeaG benefits N starved E. coli remains elusive. By combining transcriptomics with targeted metabolomics analyses, we demonstrate that the methionine biosynthesis pathway becomes transcriptionally dysregulated in ΔyeaG bacteria experiencing sustained N starvation. It appears the ability of MetJ, the master transcriptional repressor of methionine biosynthesis genes, to effectively repress transcription of genes under its control is compromised in ΔyeaG bacteria under sustained N starvation, resulting in transcriptional derepression of MetJ-regulated genes. Although the aberrant biosynthesis does not appear to be a contributing factor for the compromised viability of ΔyeaG bacteria experiencing sustained N starvation, this study identifies YeaG as a novel regulatory factor in E. coli affecting the transcription of methionine biosynthesis genes under sustained N starvation.
AU  - Switzer,A
AU  - Evangelopoulos,D
AU  - Figueira,R
AU  - Carvalho,L
AU  - Brown,D
AU  - Wigneshweraraj,S
DO  - 10.1099/mic.0.000683
EP  - 1470
PY  - 2018///
SN  - 1350-0872
SP  - 1457
TI  - A novel regulatory factor affecting the transcription of methionine biosynthesis genes in Escherichia coli experiencing sustained nitrogen starvation
T2  - Microbiology
UR  - http://dx.doi.org/10.1099/mic.0.000683
UR  - https://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000683
UR  - http://hdl.handle.net/10044/1/60836
VL  - 164
ER  -
Download