Imperial College London
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Professor Ramesh Wigneshweraraj

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 1867s.r.wig

 
 
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Location

 

4.40BFlowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Switzer:2018:10.1101/254623,
author = {Switzer, A and Evangelopoulos, D and Figueira, R and de, Carvalho LPS and Brown, DR and Wigneshweraraj, S},
doi = {10.1101/254623},
title = {A role for a conserved kinase in the transcriptional control of methionine biosynthesis in<i>Escherichia coli</i>experiencing sustained nitrogen starvation},
url = {http://dx.doi.org/10.1101/254623},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>ABSTRACT</jats:title><jats:p>The initial adaptive transcriptional response to nitrogen (N) starvation in<jats:italic>Escherichia coli</jats:italic>involves large-scale alterations to the transcriptome mediated by the transcription activator, NtrC. One of the NtrC-activated genes is<jats:italic>yeaG</jats:italic>, which encodes a conserved bacterial kinase. Although it is known that YeaG is required for optimal survival under sustained N starvation, the molecular basis by which YeaG benefits N starved<jats:italic>E. coli</jats:italic>remains elusive. By combining transcriptomics with targeted metabolomics analyses, we demonstrate that the methionine biosynthesis pathway becomes transcriptionally dysregulated in<jats:italic>ΔyeaG</jats:italic>bacteria experiencing sustained N starvation. This results in the aberrant and energetically costly biosynthesis of methionine and associated metabolites under sustained N starvation with detrimental consequences to cell viability. It appears the activity of the master transcriptional repressor of methionine biosynthesis genes, MetJ, is compromised in<jats:italic>ΔyeaG</jats:italic>bacteria under sustained N starvation, resulting in transcriptional derepression of MetJ-regulated genes. The results suggest that YeaG is a novel regulatory factor and functions as a molecular brake in the transcriptional control of both the NtrC-regulon and methionine biosynthesis genes in<jats:italic>E. coli</jats:italic>experiencing sustained N starvation.</jats:p>
AU  - Switzer,A
AU  - Evangelopoulos,D
AU  - Figueira,R
AU  - de,Carvalho LPS
AU  - Brown,DR
AU  - Wigneshweraraj,S
DO  - 10.1101/254623
PY  - 2018///
TI  - A role for a conserved kinase in the transcriptional control of methionine biosynthesis in<i>Escherichia coli</i>experiencing sustained nitrogen starvation
UR  - http://dx.doi.org/10.1101/254623
ER  -
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