Imperial College London
Alternate

ProfessorSaraRankin

Faculty of Medicine, National Heart & Lung Institute

Professor of Leukocyte and Stem Cell Biology
 
 
 
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Contact

 

+44 (0)20 7594 3172s.rankin

 
 
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Assistant

 

Ms Georgina Moss +44 (0)20 7594 2151

 
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Location

 

Office no. 351Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Iordachescu:2018:10.1002/adbi.201700156,
author = {Iordachescu, A and Amin, HD and Rankin, SM and Williams, RL and Yapp, C and Bannerman, A and Pacureanu, A and Addison, O and Hulley, PA and Grover, LM},
doi = {10.1002/adbi.201700156},
journal = {Advanced Biosystems},
title = {An In vitro model for the development of mature bone containing an osteocyte network},
url = {http://dx.doi.org/10.1002/adbi.201700156},
volume = {2},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bone is a dynamic tissue that remodels continuously in response to local mechanical and chemical stimuli. This process can also result in maladaptive ectopic bone in response to injury, yet pathological differences at the molecular and structural levels are poorly understood. A number of in vivo models exist but can often be too complex to allow isolation of factors which may stimulate disease progression. A selfstructuring model of bone formation is presented using a fibrin gel cast between two calcium phosphate ceramic anchors. Femoral periosteal cells, seeded into these structures, deposit an ordered matrix that closely resembles mature bone in terms of chemistry (collagen:mineral ratio) and structure, which is adapted over a period of one year in culture. Raman spectroscopy and Xray diffraction confirm that the mineral is hydroxyapatite associated with collagen. Secondharmonic imaging demonstrates that collagen is organized similarly to mature mouse femora. Remarkably, cells differentiated to the osteocyte phase are linked by canaliculi (as demonstrated with nanocomputed tomography) and remained viable over the full year of culture. It is demonstrated that novel drugs can prevent ossification in constructs. This model can be employed to study bone formation in an effort to encourage or prevent ossification in a range of pathologies.
AU  - Iordachescu,A
AU  - Amin,HD
AU  - Rankin,SM
AU  - Williams,RL
AU  - Yapp,C
AU  - Bannerman,A
AU  - Pacureanu,A
AU  - Addison,O
AU  - Hulley,PA
AU  - Grover,LM
DO  - 10.1002/adbi.201700156
PY  - 2018///
SN  - 2366-7478
TI  - An In vitro model for the development of mature bone containing an osteocyte network
T2  - Advanced Biosystems
UR  - http://dx.doi.org/10.1002/adbi.201700156
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000446967100002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/71853
VL  - 2
ER  -
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