Imperial College London
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DrSophieRutschmann

Faculty of Medicine, Department of Immunology and Inflammation

Reader in Immunology
 
 
 
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Contact

 

s.rutschmann Website

 
 
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Location

 

9N4bHammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Jiang:2006:10.1073/pnas.0603804103,
author = {Jiang, Z and Georgel, P and Li, C and Choe, J and Crozat, K and Rutschmann, S and Du, X and Bigby, T and Mudd, S and Sovath, S and Wilson, IA and Olson, A and Beutler, B},
doi = {10.1073/pnas.0603804103},
journal = {Proc Natl Acad Sci U S A},
pages = {10961--10966},
title = {Details of Toll-like receptor:adapter interaction revealed by germ-line mutagenesis.},
url = {http://dx.doi.org/10.1073/pnas.0603804103},
volume = {103},
year = {2006}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The immunovariant N-ethyl-N-nitrosourea-induced mutations Pococurante (Poc) and Lackadaisical were found to alter MyD88, creating striking receptor-selective effects. Poc, in particular, prevented sensing of all MyD88-dependent Toll-like receptor (TLR) ligands except diacyl lipopeptides. Furthermore, Poc-site and classical BB loop mutations caused equivalent phenotypes when engrafted into any TLR/IL-1 receptor/resistance (TIR) domain. These observations, complemented by data from docking studies and site-directed mutagenesis, revealed that BB loops and Poc sites interact homotypically across the receptor:adapter signaling interface, whereas the C-terminal alpha(E)-helices support adapter:adapter and receptor:receptor oligomerization. We have thus defined the TIR domain surface that mediates association between TLRs and MyD88 and the surface required for MyD88 or TLR oligomerization. Moreover, MyD88 engages individual TLRs differently, suggesting the feasibility of selective pharmacologic TIR domain receptor blockade.
AU  - Jiang,Z
AU  - Georgel,P
AU  - Li,C
AU  - Choe,J
AU  - Crozat,K
AU  - Rutschmann,S
AU  - Du,X
AU  - Bigby,T
AU  - Mudd,S
AU  - Sovath,S
AU  - Wilson,IA
AU  - Olson,A
AU  - Beutler,B
DO  - 10.1073/pnas.0603804103
EP  - 10966
PY  - 2006///
SN  - 0027-8424
SP  - 10961
TI  - Details of Toll-like receptor:adapter interaction revealed by germ-line mutagenesis.
T2  - Proc Natl Acad Sci U S A
UR  - http://dx.doi.org/10.1073/pnas.0603804103
UR  - https://www.ncbi.nlm.nih.gov/pubmed/16832055
VL  - 103
ER  -
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