Imperial College London


Faculty of EngineeringDepartment of Bioengineering

Professor of Neurotechnology



+44 (0)20 7594 1533s.schultz Website




4.11Royal School of MinesSouth Kensington Campus






BibTex format

author = {Quicke, P and Song, C and McKimm, EJ and Milosevic, MM and Howe, CL and Neil, M and Schultz, SR and Antic, SD and Foust, AJ and Knopfel, T},
doi = {10.3389/fncel.2019.00039},
journal = {Frontiers in Cellular Neuroscience},
title = {Single-neuron level one-photon voltage imaging with sparsely targeted genetically encoded voltage indicators},
url = {},
volume = {13},
year = {2019}

RIS format (EndNote, RefMan)

AB - Voltage imaging of many neurons simultaneously at single-cell resolution is hampered by the difficulty of detecting small voltage signals from overlapping neuronal processes in neural tissue. Recent advances in genetically encoded voltage indicator (GEVI) imaging have shown single-cell resolution optical voltage recordings in intact tissue through imaging naturally sparse cell classes, sparse viral expression, soma restricted expression, advanced optical systems, or a combination of these. Widespread sparse and strong transgenic GEVI expression would enable straightforward optical access to a densely occurring cell type, such as cortical pyramidal cells. Here we demonstrate that a recently described sparse transgenic expression strategy can enable single-cell resolution voltage imaging of cortical pyramidal cells in intact brain tissue without restricting expression to the soma. We also quantify the functional crosstalk in brain tissue and discuss optimal imaging rates to inform future GEVI experimental design.
AU - Quicke,P
AU - Song,C
AU - McKimm,EJ
AU - Milosevic,MM
AU - Howe,CL
AU - Neil,M
AU - Schultz,SR
AU - Antic,SD
AU - Foust,AJ
AU - Knopfel,T
DO - 10.3389/fncel.2019.00039
PY - 2019///
SN - 1662-5102
TI - Single-neuron level one-photon voltage imaging with sparsely targeted genetically encoded voltage indicators
T2 - Frontiers in Cellular Neuroscience
UR -
UR -
VL - 13
ER -