Imperial College London

ProfessorSimonSchultz

Faculty of EngineeringDepartment of Bioengineering

Professor of Neurotechnology
 
 
 
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Contact

 

+44 (0)20 7594 1533s.schultz Website

 
 
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Location

 

4.11Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Annecchino:2018:10.1177/2398212818776561,
author = {Annecchino, L and Schultz, SR},
doi = {10.1177/2398212818776561},
journal = {Brain and Neuroscience Advances},
pages = {1--16},
title = {Progress in automating patch clamp cellular physiology},
url = {http://dx.doi.org/10.1177/2398212818776561},
volume = {2},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Patch clamp electrophysiology has transformed research in the life sciences over the last few decades. Since theirinception, automatic patch clamp platforms have evolved considerably, demonstrating the capability to address bothvoltage and ligand gated channels, and showing the potential to play a pivotal role in drug discovery and biomedicalresearch. Unfortunately, the cell suspension assays to which early systems were limited cannot recreate biologicallyrelevant cellular environments, or capture higher-order aspects of synaptic physiology and network dynamics. In vivopatch clamp electrophysiology has the potential to yield more biologically complex information and be especially usefulin reverse engineering the molecular and cellular mechanisms of single-cell and network neuronal computation, whilecapturing important aspects of human disease mechanisms and possible therapeutic strategies. Unfortunately, it isa difficult procedure with a steep learning curve, which has restricted dissemination of the technique. Luckily, Invivo patch clamp electrophysiology seems particularly amenable to robotic automation. In this review, we documentthe development of automated patch clamp technology, from early systems based on multi-well plates through toautomated planar array platforms, and modern robotic platforms capable of performing two-photon targeted whole-cellelectrophysiological recordings in vivo.
AU - Annecchino,L
AU - Schultz,SR
DO - 10.1177/2398212818776561
EP - 16
PY - 2018///
SN - 2398-2128
SP - 1
TI - Progress in automating patch clamp cellular physiology
T2 - Brain and Neuroscience Advances
UR - http://dx.doi.org/10.1177/2398212818776561
UR - http://hdl.handle.net/10044/1/59232
VL - 2
ER -