Imperial College London
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ProfessorShiraneeSriskandan

Faculty of Medicine, Department of Infectious Disease

Professor of Infectious Diseases
 
 
 
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Contact

 

s.sriskandan

 
 
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Assistant

 

Ms Teyanna Gaeta +44 (0)20 3313 1943

 
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Location

 

8N21ACWBCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Afshar:2017:10.1080/21505594.2017.1325070,
author = {Afshar, B and Turner, CE and Lamagni, TL and Smith, K and Al-Shahib, A and Underwood, A and Holden, MTG and Efstratiou, A and Sriskandan, S},
doi = {10.1080/21505594.2017.1325070},
journal = {Virulence},
pages = {1390--1400},
title = {Enhanced nasopharyngeal infection and shedding associated with an epidemic lineage of emm3 group A Streptococcus},
url = {http://dx.doi.org/10.1080/21505594.2017.1325070},
volume = {8},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: A group A Streptococcus (GAS) lineage of genotype emm3, sequence type 15 (ST15) was associated with a 6 month upsurge in invasive GAS disease in the UK. The epidemic lineage (Lineage C) had lost 2 typical emm3 prophages, Φ315.1 and Φ315.2 associated with the superantigen ssa, but gained a different prophage (ΦUK-M3.1) associated with a different superantigen, speC and a DNAse spd1. Methods and Results: The presence of speC and spd1 in Lineage C ST15 strains enhanced both in vitro mitogenic and DNase activities over non-Lineage C ST15 strains. Invasive disease models in Galleria mellonella and SPEC-sensitive transgenic mice, revealed no difference in overall invasiveness of Lineage C ST15 strains compared with non-Lineage C ST15 strains, consistent with clinical and epidemiological analysis. Lineage C strains did however markedly prolong murine nasal infection with enhanced nasal and airborne shedding compared with non-Lineage C strains. Deletion of speC or spd1 in 2 Lineage C strains identified a possible role for spd1 in airborne shedding from the murine nasopharynx. Conclusions: Nasopharyngeal infection and shedding of Lineage C strains was enhanced compared with non-Lineage C strains and this was, in part, mediated by the gain of the DNase spd1 through prophage acquisition.
AU  - Afshar,B
AU  - Turner,CE
AU  - Lamagni,TL
AU  - Smith,K
AU  - Al-Shahib,A
AU  - Underwood,A
AU  - Holden,MTG
AU  - Efstratiou,A
AU  - Sriskandan,S
DO  - 10.1080/21505594.2017.1325070
EP  - 1400
PY  - 2017///
SN  - 2150-5608
SP  - 1390
TI  - Enhanced nasopharyngeal infection and shedding associated with an epidemic lineage of emm3 group A Streptococcus
T2  - Virulence
UR  - http://dx.doi.org/10.1080/21505594.2017.1325070
UR  - http://hdl.handle.net/10044/1/48335
VL  - 8
ER  -
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