Imperial College London
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ProfessorShiraneeSriskandan

Faculty of Medicine, Department of Infectious Disease

Professor of Infectious Diseases
 
 
 
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Contact

 

s.sriskandan

 
 
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Assistant

 

Ms Teyanna Gaeta +44 (0)20 3313 1943

 
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Location

 

8N21ACWBCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Edwards:2018:10.1038/s41598-018-24216-2,
author = {Edwards, RJ and Pyzio, M and Gierula, M and Turner, CE and Abdul-Salam, VB and Sriskandan, S},
doi = {10.1038/s41598-018-24216-2},
journal = {Scientific Reports},
title = {Proteomic analysis at the sites of clinical infection with invasive Streptococcus pyogenes},
url = {http://dx.doi.org/10.1038/s41598-018-24216-2},
volume = {8},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Invasive Streptococcus pyogenes infections are rare, with often-unexplained severity. Prompt diagnosis is desirable, as deaths can occur rapidly following onset and there is an increased, but preventable, risk to contacts.  Here, proteomic analyses of clinical samples from invasive human S. pyogenes infections were undertaken to determine if novel diagnostic targets could be detected, and to augment our understanding of disease pathogenesis. Fluid samples from 17 patients with confirmed invasive S. pyogenes infection (empyema, septic arthritis, necrotising fasciitis) were analysed by proteomics for streptococcal and human proteins; 16/17 samples had detectable S. pyogenes DNA. Nineteen unique S. pyogenes proteins were identified in just 6/17 samples, and 15 of these were found in a single pleural fluid sample including streptococcal inhibitor of complement, trigger factor, and phosphoglycerate kinase. In contrast, 469 human proteins were detected in patient fluids, 177 (38%) of which could be identified as neutrophil proteins, including alpha enolase and lactotransferrin which, together, were found in all 17 samples. Our data suggest that streptococcal proteins are difficult to detect in infected fluid samples. A vast array of human proteins associated with leukocyte activity are, however, present in samples that deserve further evaluation as potential biomarkers of infection.
AU  - Edwards,RJ
AU  - Pyzio,M
AU  - Gierula,M
AU  - Turner,CE
AU  - Abdul-Salam,VB
AU  - Sriskandan,S
DO  - 10.1038/s41598-018-24216-2
PY  - 2018///
SN  - 2045-2322
TI  - Proteomic analysis at the sites of clinical infection with invasive Streptococcus pyogenes
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-018-24216-2
UR  - http://hdl.handle.net/10044/1/58441
VL  - 8
ER  -
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