Publications

BibTex format

@article{Saint:2019:10.1038/s41564-018-0330-4,
author = {Saint, M and Bertaux, F and Tang, W and Sun, X-M and Game, L and Köferle, A and Bähler, J and Shahrezaei, V and Marguerat, S},
doi = {10.1038/s41564-018-0330-4},
journal = {Nature Microbiology},
pages = {480--491},
title = {Single-cell imaging and RNA sequencing reveal patterns of gene expression heterogeneity during fission yeast growth and adaptation},
url = {http://dx.doi.org/10.1038/s41564-018-0330-4},
volume = {4},
year = {2019}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Phenotypic cell-to-cell variability is a fundamental determinant of microbial fitness that contributes to stress adaptation and drug resistance. Gene expression heterogeneity underpins this variability but is challenging to study genome-wide. Here we examine the transcriptomes of >2,000 single fission yeast cells exposed to various environmental conditions by combining imaging, single-cell RNA sequencing and Bayesian true count recovery. We identify sets of highly variable genes during rapid proliferation in constant culture conditions. By integrating single-cell RNA sequencing and cell-size data, we provide insights into genes that are regulated during cell growth and division, including genes whose expression does not scale with cell size. We further analyse the heterogeneity of gene expression during adaptive and acute responses to changing environments. Entry into the stationary phase is preceded by a gradual, synchronized adaptation in gene regulation that is followed by highly variable gene expression when growth decreases. Conversely, sudden and acute heat shock leads to a stronger, coordinated response and adaptation across cells. This analysis reveals that the magnitude of global gene expression heterogeneity is regulated in response to different physiological conditions within populations of a unicellular eukaryote.
AU  - Saint,M
AU  - Bertaux,F
AU  - Tang,W
AU  - Sun,X-M
AU  - Game,L
AU  - Köferle,A
AU  - Bähler,J
AU  - Shahrezaei,V
AU  - Marguerat,S
DO  - 10.1038/s41564-018-0330-4
EP  - 491
PY  - 2019///
SN  - 2058-5276
SP  - 480
TI  - Single-cell imaging and RNA sequencing reveal patterns of gene expression heterogeneity during fission yeast growth and adaptation
T2  - Nature Microbiology
UR  - http://dx.doi.org/10.1038/s41564-018-0330-4
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30718845
UR  - http://hdl.handle.net/10044/1/66903
VL  - 4
ER  -

Download

DrSamuelMarguerat

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)