Imperial College London
Dr Samuel Marguerat

DrSamuelMarguerat

Faculty of Natural Sciences, Department of Mathematics

Visiting Researcher
 
 
 
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Contact

 

+44 (0)20 3313 8331samuel.marguerat Website

 
 
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Location

 

5003CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Marguerat:2014:10.4161/rna.29196,
author = {Marguerat, S and Lawler, K and Brazma, A and Baehler, J},
doi = {10.4161/rna.29196},
journal = {RNA Biology},
pages = {702--714},
title = {Contributions of transcription and mRNA decay to gene expression dynamics of fission yeast in response to oxidative stress},
url = {http://dx.doi.org/10.4161/rna.29196},
volume = {11},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The cooperation of transcriptional and post-transcriptional levels of control to shape gene regulation is only partially understood. Here we show that a combination of two simple and non-invasive genomic techniques, coupled with kinetic mathematical modeling, affords insight into the intricate dynamics of RNA regulation in response to oxidative stress in the fission yeast Schizosaccharomyces pombe. This study reveals a dominant role of transcriptional regulation in response to stress, but also points to the first minutes after stress induction as a critical time when the coordinated control of mRNA turnover can support the control of transcription for rapid gene regulation. In addition, we uncover specialized gene expression strategies associated with distinct functional gene groups, such as simultaneous transcriptional repression and mRNA destabilization for genes encoding ribosomal proteins, delayed mRNA destabilization with varying contribution of transcription for ribosome biogenesis genes, dominant roles of mRNA stabilization for genes functioning in protein degradation, and adjustment of both transcription and mRNA turnover during the adaptation to stress. We also show that genes regulated independently of the bZIP transcription factor Atf1p are predominantly controlled by mRNA turnover, and identify putative cis-regulatory sequences that are associated with different gene expression strategies during the stress response. This study highlights the intricate and multi-faceted interplay between transcription and RNA turnover during the dynamic regulatory response to stress.
AU  - Marguerat,S
AU  - Lawler,K
AU  - Brazma,A
AU  - Baehler,J
DO  - 10.4161/rna.29196
EP  - 714
PY  - 2014///
SN  - 1547-6286
SP  - 702
TI  - Contributions of transcription and mRNA decay to gene expression dynamics of fission yeast in response to oxidative stress
T2  - RNA Biology
UR  - http://dx.doi.org/10.4161/rna.29196
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000341971700009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.tandfonline.com/doi/full/10.4161/rna.29196
UR  - http://hdl.handle.net/10044/1/77137
VL  - 11
ER  -
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