Publications
464 results found
Chen QZ, Harding SE, Rai R, et al., 2014, Myocardial tissue engineering, Tissue Engineering Using Ceramics and Polymers: Second Edition, Pages: 387-413, ISBN: 9780857097125
In the pursuit of engineering a functional myocardium, studies integrating highly innovative research in stem cells and biomaterials development have opened new frontiers. Stringent properties like biocompatibility, ability to foster cells, biodegradability and mechanical compliance have been the norm for material selection. Fabrication techniques implemented individually or in conjunction have been used to fabricate scaffolds that best mimic the anisotropic nature of the cardiac extracellular matrix (ECM). Such biomimetic scaffolds provide topographical cues for contact guidance to align cells required to achieve synchronous electrophysiological coupling of the engineered myocardium to the native heart. Various functionalization strategies in tandem with scaffold fabrication designs and bioreactor studies have been undertaken to achieve vascularization in the engineered cardiac construct.
Sikkel MB, Hayward C, MacLeod KT, et al., 2014, SERCA2a gene therapy in heart failure: an anti-arrhythmic positive inotrope, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 171, Pages: 38-54, ISSN: 0007-1188
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- Citations: 30
Schobesberger S, Tokar S, Bhargava A, et al., 2013, Alteration in b2-ARs Dependent cAMP Signalling Linked to Post Infarction Remodelling of T-tubules in Rat Cardiomyocytes, Scientific Sessions and Resuscitation Science Symposium of the American-Heart-Association, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Harding S, 2013, Heart failure therapy: drugs, genes or stem cells?: by Caroline Telfer., Future Cardiol, Vol: 9, Pages: 629-633
Professor Sian Harding talks to Caroline Telfer, Assistant Commissioning Editor. Professor Sian Harding obtained her PhD in Pharmacology from King's College, London (UK) in 1981. She became Professor of Cardiac Pharmacology at the National Heart and Lung Institute, a division of the Imperial College Faculty of Medicine, in 2002. Her work has been funded by the British Heart Foundation, the Wellcome Trust, the Medical Research Council, the Biochemical and Biophysical Research Council, the The National Centre for the Replacement, Refinement and Reduction of Animals in Research, Pfizer, GlaxoSmithKline and SmithKline Beecham. Harding is former president of the European Section of the International Society for Heart Research and has organized international cardiovascular science meetings for this society, as well as for the European Society of Cardiology. She is the principal investigator for the first UK gene therapy trial aimed at improving cardiac contractility, organized jointly at Harefield and Papworth Hospitals. Harding is a member of the Nuffield Council on Bioethics and the Medical Research Coucil Regenerative Medicine Research Committee, and Director of a recently awarded British Heart Foundation Cardiovascular Regenerative Medicine Centre. She has been elected Fellow of the American Heart Association, European Society of Cardiology, International Society for Heart Research, Society of Biology and British Society of Pharmacology.
Song X, Xie M, Hofmann F, et al., 2013, Residual stresses in Linear Friction Welding of aluminium alloys, Materials & Design, Vol: 50, Pages: 360-369, ISSN: 0261-3069
Foldes G, Matsa E, Kriston-Vizi J, et al., 2013, Key differences in hypertrophic signalling between hESC- and hIPSC-derived cardiomyocytes, Congress of the European-Society-of-Cardiology (ESC), Publisher: OXFORD UNIV PRESS, Pages: 279-279, ISSN: 0195-668X
Rees PSC, Davidson SM, Harding SE, et al., 2013, The Mitochondrial Permeability Transition Pore as a Target for Cardioprotection in Hypertrophic Cardiomyopathy, CARDIOVASCULAR DRUGS AND THERAPY, Vol: 27, Pages: 235-237, ISSN: 0920-3206
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- Citations: 5
Gorelik J, Wright PT, Lyon AR, et al., 2013, Spatial control of the βAR system in heart failure: the transverse tubule and beyond, CARDIOVASCULAR RESEARCH, Vol: 98, Pages: 216-224, ISSN: 0008-6363
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- Citations: 36
Sikkel MB, Collins TP, Rowlands C, et al., 2013, Triple mode of action of flecainide in catecholaminergic polymorphic ventricular tachycardia: reply, CARDIOVASCULAR RESEARCH, Vol: 98, Pages: 327-328, ISSN: 0008-6363
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- Citations: 10
Sikkel MB, Collins TP, Rowlands C, et al., 2013, Flecainide reduces Ca<SUP>2+</SUP> spark and wave frequency via inhibition of the sarcolemmal sodium current, CARDIOVASCULAR RESEARCH, Vol: 98, Pages: 286-296, ISSN: 0008-6363
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- Citations: 64
Sikkel M, Rowlands C, Harding S, et al., 2013, REDUCING SARCOLEMMAL SODIUM CURRENT DECREASES SPONTANEOUS SR CA2+RELEASE, Annual Conference of the British-Cardiovascular-Society (BCS), Publisher: BMJ PUBLISHING GROUP, Pages: A120-A120, ISSN: 1355-6037
Mills A, Harding S, Lyon A, et al., 2013, MiR-1 and SERCA2a gene therapy in the failing heart, Conference of the British-Society-for-Gene-and-Cell-Therapy (BSGCT), Publisher: MARY ANN LIEBERT INC, Pages: A34-A34, ISSN: 1043-0342
Foldes G, Matsa E, Kriston-Vizi J, et al., 2013, Key differences in hypertrophic signalling in hESC-and hIPSC-derived cardiomyocytes, Conference of the British-Society-for-Gene-and-Cell-Therapy (BSGCT), Publisher: MARY ANN LIEBERT INC, Pages: A9-A9, ISSN: 1043-0342
Khan JM, Lyon AR, Harding SE, 2013, The case for induced pluripotent stem cell-derived cardiomyocytes in pharmacological screening, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 169, Pages: 304-317, ISSN: 0007-1188
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- Citations: 53
Reed DM, Kirkby NS, Foldes G, et al., 2013, Prostacyclin release pathways in stem cell derived endothelial cells, Experimental Biology 2013
Reed DM, Foldes G, Gashaw HH, et al., 2013, Conditioning of human embryonic stem cell-derived endothelial cells with PBMCs confers TLR4 sensing in co-culture conditions, Experimental Biology 2013
Gillis RB, Adams GG, Wolf B, et al., 2013, Molecular weight distribution analysis by ultracentrifugation: Adaptation of a new approach for mucins, 2nd International Polysaccharide Conference on Polysaccharides as Source of Advanced and Sustainable Products (EPNOE), Publisher: ELSEVIER SCI LTD, Pages: 178-183, ISSN: 0144-8617
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- Citations: 13
Rao C, Prodromakis T, Kolker L, et al., 2013, The effect of microgrooved culture substrates on calcium cycling of cardiac myocytes derived from human induced pluripotent stem cells, Biomaterials, Vol: 34, Pages: 2399-2411, ISSN: 0142-9612
Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) have been widely proposed as in vitro models of myocardial physiology and disease. A significant obstacle, however, is their immature phenotype. We hypothesised that Ca2+ cycling of iPSC-CM is influenced by culture conditions and can be manipulated to obtain a more mature cellular behaviour. To test this hypothesis we seeded iPSC-CM onto fibronectin coated microgrooved polydimethylsiloxane (PDMS) scaffolds fabricated using photolithography, or onto unstructured PDMS membrane. After two weeks in culture, the structure and function of iPSC-CM were studied. PDMS microgrooved culture substrates brought about cellular alignment (p < 0.0001) and more organised sarcomere. The Ca2+ cycling properties of iPSC-CM cultured on these substrates were significantly altered with a shorter time to peak amplitude (p = 0.0002 at 1 Hz), and more organised sarcoplasmic reticulum (SR) Ca2+ release in response to caffeine (p < 0.0001), suggesting improved SR Ca2+ cycling. These changes were not associated with modifications in gene expression. Whilst structured tissue culture may make iPSC-CM more representative of adult myocardium, further construct development and characterisation is required to optimise iPSC-CM as a model of adult myocardium.
Roether JA, Jawad H, Rai R, et al., 2013, Polymers for Myocardial Tissue Engineering, POLYMERIC BIOMATERIALS: MEDICINAL AND PHARMACEUTICAL APPLICATIONS, VOL 2, Editors: Popa, Publisher: CRC PRESS-TAYLOR & FRANCIS GROUP, Pages: 369-388, ISBN: 978-1-4200-9468-8
Wright PT, Diakonov I, Nikolaev VO, et al., 2012, Myocardial Gradients in Caveolar Number Modulate Cardiomyocyte Contractile Response to Specific β2AR Stimulation, CIRCULATION, Vol: 126, ISSN: 0009-7322
Wright PT, Nikolaev VO, Tokar S, et al., 2012, Caveolin3 Depletion Modifies β2AR-cAMP Responses at Both Cellular and Sub-Cellular Levels in Cardiomyocytes, CIRCULATION, Vol: 126, ISSN: 0009-7322
Mioulane M, Foldes G, Ali NN, et al., 2012, Development of high content imaging methods for cell death detection in human pluripotent stem cell-derived cardiomyocytes, Journal of Cardiovascular Translational Research, Vol: 5, Pages: 593-604, ISSN: 1937-5387
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) are being investigated as a new source of cardiac cells for drug safety assessment. We developed a novel scalable high content microscopy-based method for the detection of cell death in hPSC-CM that can serve for future predictive in vitro cardio-toxicological screens. Using rat neonatal ventricular cardiomyocytes (RVNC) or hPSC-CM, assays for nuclear remodelling, mitochondrial status, apoptosis and necrosis were designed using a combination of fluorescent dyes and antibodies on an automated microscopy platform. This allowed the observation of a chelerythrine-induced concentration-dependent apoptosis to necrosis switch and time-dependent progression of early apoptotic cells towards a necrotic-like phenotype. Susceptibility of hPSC-CM to chelerythrine-stimulated apoptosis varied with time after differentiation, but at most time points, hPSC-CM were more resistant than RVNC. This simple and scalable humanized high-content assay generates accurate cardiotoxicity profiles that can serve as a base for further assessment of cardioprotective strategies and drug safety.
Harding SE, 2012, Frontiers in cardiovascular biology: London 2012 - a scientific 'olympiad'., Future Cardiol, Vol: 8, Pages: 689-691
Imperial College London (UK) was the showcase for the second in the 'Frontiers in Cardiovascular Biology' series, a biennial meeting of the European Society of Cardiology Council on basic cardiovascular sciences, held from 30 March to 1 April 2012. The aim of this series is to bring researchers together to learn the very latest findings in cardiac and vascular sciences, and to see state-of-the-art and developing technologies that could impact cardiovascular research. Five keynote lectures, 25 scientific symposia and two translational lunchtime symposia were grouped around the central themes of bioimaging, degeneration and regeneration, and inflammation.
Paur H, Wright PT, Sikkel MB, et al., 2012, High levels of circulating epinephrine trigger apical cardiodepression in a β2-adrenergic receptor/Gi-dependent manner: a new model of Takotsubo cardiomyopathy., Circulation Journal, Vol: 6, Pages: 697-706
Mioulane M, Foldes G, Harding S, 2012, Cardiomyocytes from Human Pluripotent Stem Cells Predict Chemotherapy-Induced Cardiotoxicity, Basic Cardiovascular Sciences Scientific Session, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7330
Sikkel MB, Collins TP, O'Gara P, et al., 2012, Elemental SR Ca2+release in failing human cardiomyocytes - a comparison with failing rat cardiomyocytes and inhibition by flecainide, Congress of the European-Society-of-Cardiology (ESC), Publisher: OXFORD UNIV PRESS, Pages: 1045-1045, ISSN: 0195-668X
Reed DM, Foldes G, Harding SE, et al., 2012, Stem cell derived endothelial cells for cardiovascular disease; a therapeutic perspective., British Journal of Clinical Pharmacology
Wright PT, Pannell LMK, Nikolaev VO, et al., 2012, Imaging regional differences in beta-2 adrenergic receptor function at the cellular level, BSCR/BAS Joint Spring Meeting
Shah M, Sikkel MB, Desplantez T, et al., 2012, Flecainide reduces calcium transient amplitude in rat cardiomyocytes with an absence of a significant reduction in l-type calcium current, 2nd Congress of the European-Society-of-Cardiology Council on Basic Cardiovascular Science - Frontiers in Cardiovascular Biology, Publisher: OXFORD UNIV PRESS, Pages: S108-S108, ISSN: 0008-6363
Foldes G, Bagyura Z, Lendvai Z, et al., 2012, Transplantation of human embryonic stem cell-derived endothelial cells into rats: a new approach for vascular regeneration, 2nd Congress of the European-Society-of-Cardiology Council on Basic Cardiovascular Science - Frontiers in Cardiovascular Biology, Publisher: OXFORD UNIV PRESS, Pages: S16-S17, ISSN: 0008-6363
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