Imperial College London
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ProfessorSianHarding

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor of Cardiac Pharmacology
 
 
 
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Contact

 

+44 (0)20 7594 3009sian.harding Website

 
 
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Location

 

435ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Yang:2017:hmg/ddx140,
author = {Yang, C and Xu, Y and Yu, M and Lee, D and Alharti, S and Hellen, N and Shaik, NA and Banaganapalli, B and Mohamoud, HSA and Elango, R and Przyborski, S and Tenin, G and Williams, S and O'Sullivan, J and Al-Radi, OO and Atta, J and Harding, SE and Keavney, B and Lako, M and Armstrong, L},
doi = {hmg/ddx140},
journal = {Human Molecular Genetics},
pages = {3031--3045},
title = {Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis.},
url = {http://dx.doi.org/10.1093/hmg/ddx140},
volume = {26},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Hypoplastic left heart syndrome (HLHS) is among the most severe forms of congenital heart disease. Although the consensus view is that reduced flow through the left heart during development is a key factor in the development of the condition, the molecular mechanisms leading to hypoplasia of left heart structures are unknown. We have generated induced pluripotent stem cells (iPSC) from five HLHS patients and two unaffected controls, differentiated these to cardiomyocytes and identified reproducible in vitro cellular and functional correlates of the HLHS phenotype. Our data indicate that HLHS-iPSC have a reduced ability to give rise to mesodermal, cardiac progenitors and mature cardiomyocytes and an enhanced ability to differentiate to smooth muscle cells. HLHS-iPSC-derived cardiomyocytes are characterised by a lower beating rate, disorganised sarcomeres and sarcoplasmic reticulum and a blunted response to isoprenaline. Whole exome sequencing of HLHS fibroblasts identified deleterious variants in NOTCH receptors and other genes involved in the NOTCH signalling pathway. Our data indicate that the expression of NOTCH receptors was significantly downregulated in HLHS-iPSC-derived cardiomyocytes alongside NOTCH target genes confirming downregulation of NOTCH signalling activity. Activation of NOTCH signalling via addition of Jagged peptide ligand during the differentiation of HLHS-iPSC restored their cardiomyocyte differentiation capacity and beating rate and suppressed the smooth muscle cell formation. Together, our data provide firm evidence for involvement of NOTCH signalling in HLHS pathogenesis, reveal novel genetic insights important for HLHS pathology and shed new insights into the role of this pathway during human cardiac development.
AU  - Yang,C
AU  - Xu,Y
AU  - Yu,M
AU  - Lee,D
AU  - Alharti,S
AU  - Hellen,N
AU  - Shaik,NA
AU  - Banaganapalli,B
AU  - Mohamoud,HSA
AU  - Elango,R
AU  - Przyborski,S
AU  - Tenin,G
AU  - Williams,S
AU  - O'Sullivan,J
AU  - Al-Radi,OO
AU  - Atta,J
AU  - Harding,SE
AU  - Keavney,B
AU  - Lako,M
AU  - Armstrong,L
DO  - hmg/ddx140
EP  - 3045
PY  - 2017///
SN  - 0964-6906
SP  - 3031
TI  - Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis.
T2  - Human Molecular Genetics
UR  - http://dx.doi.org/10.1093/hmg/ddx140
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406794000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/61162
VL  - 26
ER  -
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