Imperial College London
Alternate

Professor Suveer Singh

Faculty of Medicine, Department of Surgery & Cancer

Professor of Practice(Respiratory & Intensive Care Medicine)
 
 
 
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Contact

 

+44 (0)20 3315 8472suveer.singh Website CV

 
 
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Location

 

David Erskine WardChelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{O'Dea:2016:10.1371/journal.pone.0167801,
author = {O'Dea, KP and Porter, J and TIrlapur, N and Katbeh, U and Singh, S and Handy, JM and Takata, M},
doi = {10.1371/journal.pone.0167801},
journal = {PLOS One},
title = {Circulating microvesicles are elevated acutely following major burns injury and associated with clinical severity},
url = {http://dx.doi.org/10.1371/journal.pone.0167801},
volume = {11},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Microvesicles  are  cell-derived  signaling  particles  emerging  as  important  mediators  and  biomarkers of systemic inflammation, but their production in severe burn injury patients has not  been  described.    In  this  pilot  investigation,  we  measured  circulating  microvesicle  levels  following severe burns, with severe sepsis patients as a comparator group.We hypothesized that  levels  of  circulating  vascular  cell-derived  microvesicles  are  elevated  acutely  following  burns  injury,  mirroring  clinical  severity  due  to  the  early  onset  and  prevalence  of  systemic  inflammatory  response  syndrome  (SIRS)  in  these  patients.    Blood  samples  were  obtained  from  patients  with  moderate  to  severe  thermal  injury  burns,  with  severe  sepsis,  and  from  healthy  volunteers.  Circulating  microvesicles  derived  from  total  leukocytes,  granulocytes,  monocytes,  and  endothelial  cells  were  quantified  in  plasma  by  flow  cytometry.All circulating microvesicle subpopulations were elevated in burns patients on day of admission (day   0)   compared   to   healthy   volunteers   (leukocyte-microvesicles:   3.5-fold, p=0.005; granulocyte-microvesicles:     12.8-fold,     p<0.0001;     monocyte-microvesicles:     20.4-fold, p<0.0001; endothelial- microvesicles: 9.6-fold, p=0.01), but decreased significantly by day 2.  Microvesicle levels were increased with severe sepsis, but less consistently between patients. Leukocyte-   and   granulocyte-derived   microvesicles   on   day   0   correlated   with   clinical   assessment  scores  and  were  higher  in  burns  ICU  non-survivors  compared  to  survivors  (leukocyte MVs 4.6 fold, p=0.002; granulocyte MVs 4.8 fold, p=0.003).  Mortality prediction analysis  of  area  under  receiver  operating  characteristic  curve  was  0.92  (p=0.01)  for  total  leukocyte  microvesicles  and  0.85  (p=0.04)  for  granulocyte  microvesicles.  These  findings  demonstrate,  for  the  first  t
AU  - O'Dea,KP
AU  - Porter,J
AU  - TIrlapur,N
AU  - Katbeh,U
AU  - Singh,S
AU  - Handy,JM
AU  - Takata,M
DO  - 10.1371/journal.pone.0167801
PY  - 2016///
SN  - 1932-6203
TI  - Circulating microvesicles are elevated acutely following major burns injury and associated with clinical severity
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0167801
UR  - http://hdl.handle.net/10044/1/42847
VL  - 11
ER  -
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