Imperial College London
Alternate

Professor Suveer Singh

Faculty of Medicine, Department of Surgery & Cancer

Professor of Practice(Respiratory & Intensive Care Medicine)
 
 
 
//

Contact

 

+44 (0)20 3315 8472suveer.singh Website CV

 
 
//

Location

 

David Erskine WardChelsea and Westminster HospitalChelsea and Westminster Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Morris:2017:10.1136/thoraxjnl-2016-209065,
author = {Morris, AC and Gadsby, N and McKenna, JP and Hellyer, TP and Dark, P and Singh, S and Walsh, TS and McAuley, DF and Templeton, K and Simpson, AJ and McMullan, R},
doi = {10.1136/thoraxjnl-2016-209065},
journal = {Thorax},
pages = {1046--1048},
title = {16S pan-bacterial PCR can accurately identify patients with ventilator-associated pneumonia},
url = {http://dx.doi.org/10.1136/thoraxjnl-2016-209065},
volume = {72},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Ventilator-associated pneumonia (VAP) remains a challenge to intensive care units, with secure diagnosis relying on microbiological cultures that take up to 72 hours to provide a result. We sought to derive and validate a novel, real-time 16S rRNA gene PCR for rapid exclusion of VAP. Bronchoalveolar lavage (BAL) was obtained from two independent cohorts of patients with suspected VAP. Patients were recruited in a 2-centre derivation cohort and a 12-centre confirmation cohort. Confirmed VAP was defined as growth of >104 colony forming units/ml on semiquantitative culture and compared with a 16S PCR assay. Samples were tested from 67 patients in the derivation cohort, 10 (15%) of whom had confirmed VAP. Using cycles to cross threshold (Ct) values as the result of the 16S PCR test, the area under the receiver operating characteristic (ROC) curve (AUROC) was 0.94 (95% CI 0.86 to 1.0, p<0.0001). Samples from 92 patients were available from the confirmation cohort, 26 (28%) of whom had confirmed VAP. The AUROC for Ct in this cohort was 0.89 (95% CI 0.83 to 0.95, p<0.0001). This study has derived and assessed the diagnostic accuracy of a novel application for 16S PCR. This suggests that 16S PCR in BAL could be used as a rapid test in suspected VAP and may allow better stewardship of antibiotics.Trial registration number VAPRAPID trial ref NCT01972425.
AU  - Morris,AC
AU  - Gadsby,N
AU  - McKenna,JP
AU  - Hellyer,TP
AU  - Dark,P
AU  - Singh,S
AU  - Walsh,TS
AU  - McAuley,DF
AU  - Templeton,K
AU  - Simpson,AJ
AU  - McMullan,R
DO  - 10.1136/thoraxjnl-2016-209065
EP  - 1048
PY  - 2017///
SN  - 1468-3296
SP  - 1046
TI  - 16S pan-bacterial PCR can accurately identify patients with ventilator-associated pneumonia
T2  - Thorax
UR  - http://dx.doi.org/10.1136/thoraxjnl-2016-209065
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000413364800012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/67490
VL  - 72
ER  -
Download