Imperial College London

Professor Tony Cass

Faculty of Natural SciencesDepartment of Chemistry

Professor of Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Publication Type
Year
to

267 results found

Winkle RF, Nagy JM, Cass AEG, Sharma Set al., 2008, Towards microfluidic technology-based MALDI-MS platforms for drug discovery: a review, EXPERT OPINION ON DRUG DISCOVERY, Vol: 3, Pages: 1281-1292, ISSN: 1746-0441

Journal article

Nagy J, Kang Y, Cass A, Mantalaris Aet al., 2008, Finding proteins in cell-conditioned media, Genetic Engineering and Biotechnology News, Vol: 28, ISSN: 1935-472X

Journal article

Pereira F, Hassard S, Cass A, Nagy JMet al., 2008, CZE of peptides using a multi-pixel detector array, Chimica Oggi, Vol: 26, Pages: 40-42, ISSN: 0392-839X

Capillary electrophoresis has been used in the analysis of peptides for decades, however traditional methods show excessive variability due to composition and sequence of the peptide, and variation in the interaction of the peptide with the separation medium. Multi-pixel detection along with the associated data analysis software improves reproducibility and sensitivity beyond current methods using UV absorption detection. The reproducibility of the data and the sensitivity of this 'no-label' multi-pixel detection approach are investigated using a mixture of known peptides. In the near future this will enable peptide mass finger printing based on the ability to assign molecular weight values to the peptides separated by charge.

Journal article

Pereira F, Hassard S, Cass A, Nagy JMet al., 2008, CZE of peptides using a multi-pixel detector array, CHIMICA OGGI-CHEMISTRY TODAY, Vol: 26, Pages: 40-42, ISSN: 0392-839X

Journal article

Lim M, Ye H, Drakakis EM, Yue X, Cass AEG, Panoskaltsis N, Mantalaris Aet al., 2008, Towards information-rich bioprocessing: Generation of spatio-temporal profiles through the use of design of experiments to determine optimal number and location of sensors - An example in thermal profiles, Biochemical Engineering Journal, Vol: 40, Pages: 1-7, ISSN: 1369-703X

Journal article

New SEP, Chester AH, Sharma S, Safinia L, Cass AEG, Schaffer MSP, Yacoub MH, Taylor PMet al., 2008, The effect of carbon nanotubes on mesenchymal stem cells in a 3D environment, Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, Publisher: MARY ANN LIEBERT INC, Pages: 912-912, ISSN: 1937-3341

Conference paper

Patel BA, Arundell M, Quek RGW, Harvey SLR, Ellis IR, Florence MM, Cass AEG, Schor AM, O'Hare Det al., 2008, Individually addressable microelectrode array for monitoring oxygen and nitric oxide release, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol: 390, Pages: 1379-1387, ISSN: 1618-2642

Journal article

White SL, Chadban SJ, Jan S, Chapman JR, Cass Aet al., 2008, How can we achieve global equity in provision of renal replacement therapy?, Bull World Health Organ, Vol: 86, Pages: 229-237

There is a significant emerging burden of chronic and end-stage kidney disease in low- and middle-income countries, driven by population ageing and the global epidemic of type 2 diabetes. Sufferers of end-stage kidney disease require ongoing dialysis or kidney transplantation to survive; however, in many low- and middle-income countries, treatment options are strictly limited or unaffordable. Low numbers of maintenance dialysis patients and transplant recipients reflect profound economic and service provision challenges for health-care systems in low- and middle-income countries in sustaining renal replacement therapy programmes. Underdeveloped organ donor and transplant programmes, health system and financing issues, ethical regulation of transplantation and the cost of pharmaceuticals commonly pose additional barriers to the delivery of efficient and cost-effective renal replacement therapy. Development of locally appropriate transplant programmes, effective use of nongovernmental sources of funding, service planning and cost containment, use of generic drugs and local manufacture of dialysis consumables have the potential to make life-saving renal replacement therapy available to many more in need. Select low- and middle-income countries demonstrate more equitable provision of renal replacement therapy is possible outside high-income countries. For other low- and middle-income countries, education, the development of good public policy and a supportive international environment are critical. Prevention of end-stage kidney disease, ideally as part of an integrated approach to chronic vascular diseases, must also be a key objective.

Journal article

Johnson CJ, Zhukovsky N, Cass AEG, Nagy JMet al., 2008, Proteomics, nanotechnology and molecular diagnostics, PROTEOMICS, Vol: 8, Pages: 715-730, ISSN: 1615-9853

Journal article

Cass T, 2008, Biosensors, Advances in Tissue Engineering, Pages: 373-399, ISBN: 9781848161825

© 2008 by Imperial College Press. Increasingly biological sciences are being built on a quantitative foundation, based upon our ability to accurately determine the temporal and spatial variations in the concentration of key molecules. It is this quantitative analytical data that provides the testable basis for building hypotheses about biological systems. Although many, diverse analytical techniques have been used to collect quantitative data these are often destructive of the system being analysed. Biosensors by contrast promise the ability to measure selected molecules continuously and in real-time with good spatial resolution. They use specific molecular recognition at the surface of a transducer such that an electrical signal is generated in proportion to the concentration of the target analyte. Many different molecular recognition reagents have been exploited in this respect and include enzymes, binding proteins and nucleic acids, whilst electrochemical, optical and mass sensitive signal transduction devices have been used to generate the electrical signal (typically a voltage or current). Using biosensors in vivo presents additional challenges over and above simply relating signal to concentration. Biocompatibility is an ever-present issue and includes both the effect of the biological matrix on the sensor as well as sensor components on the biology. In this chapter a review of the different sensing modes is presented and their potential applicability to the monitoring of cells, tissue and tissue constructs is presented.

Book chapter

Radomska A, Singhal S, Ye H, Lim M, Mantalaris A, Yue X, Drakakis EM, Toumazou C, Cass AEGet al., 2008, Biocompatible ion selective electrode for monitoring metabolic activity during the growth and cultivation of human cells, Biosensors and Bioelectronics, Vol: 24, Pages: 435-441, ISSN: 1873-4235

Journal article

Yue X, Drakakis EM, Lim M, Radomska A, Ye H, Mantalaris A, Panoskaltsis N, Cass Aet al., 2008, A Real-Time Multi-Channel Monitoring System for Stem Cell Culture Process, IEEE Transactions on Biomedical Circuits and Systems, Vol: 2, Pages: 66-77, ISSN: 1932-4545

Journal article

Hassard J, Cass T, 2007, High-throughput monitoring of protein folding, Innovations in Pharmaceutical Technology, ISSN: 1471-7204

A novel technology has been developed for the rapid and accurate analysis of the folded state of proteins, enabling protein folding to be monitored on a high-throughput basis for drug discovery and manufacturing processes.

Journal article

Cass AEG, 2007, Organometallic compounds in biosensing, Comprehensive Organometallic Chemistry III, Pages: 589-602, ISBN: 9780080450476

Book chapter

Toumazou C, Cass T, 2007, Cell-bionics: tools for real-time sensor processing, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 362, Pages: 1321-1328, ISSN: 0962-8436

Journal article

Cass AEG, 2007, Building Biolelectronic Interfaces, Electronics Letters, Vol: 43, Pages: 903-905

Journal article

Lim M, Ye H, Panoskaltsis N, Drakakis EM, Yue X, Cass AEG, Radomska A, Mantalaris Aet al., 2007, Intelligent Bioprocessing for Haemotopoietic Cell Cultures Using Monitoring and Design of Experiments, Biotechnology Advances, Vol: 25, Pages: 353-368, ISSN: 0734-9750

Journal article

Cass A, Cunningham J, Anderson K, Snelling P, Colman S, Devitt J, Preece C, Eris Jet al., 2007, Decision-making about suitability for kidney transplantation: Results of a national survey of Australian nephrologists., Nephrology (Carlton), Vol: 12, Pages: 299-304, ISSN: 1320-5358

AIM: This study aimed to elucidate the factors affecting nephrologists' decision-making on patients' suitability for kidney transplantation. Given the reduced access to transplantation for Indigenous Australians, the role of patient's ethnicity was of particular interest. METHODS: A postal survey of practising nephrologists and trainees was undertaken in Australia. Each participant was provided with a unique set of 15 hypothetical patient descriptions, with demographic, clinical and behavioural factors randomly generated to ensure an overall balance of factors across the cases. The main outcome measure was whether kidney transplantation was recommended. RESULTS: Responding nephrologists and trainees were more likely to recommend transplantation for hypothetical patients who were young, of normal weight and described as compliant. They were less likely to recommend transplantation for smokers, or for people with diabetes or heart disease. No significant differences related to the patients' sex or ethnicity. The geographical location of the respondent was a significant determinant, with differences according to their State/Territory and their metropolitan/non-metropolitan location. CONCLUSION: When all other factors were held constant, nephrologists and trainees appear to base their decision-making regarding suitability for transplant on clinical and behavioural factors, rather than on the basis of ethnicity or sex. In practice, however, clinical and behavioural factors cluster with ethnicity, and this is likely to contribute to the current poor access to transplantation for Indigenous end-stage kidney disease patients. Apparent differences in decision-making according to the respondent's location may reflect variations in practice across the country.

Journal article

Huang X, Zhang X-E, Zhou Y-F, Zhang Z-P, Cass AEGet al., 2007, Construction of a high sensitive Escherichia coli alkaline phosphatase reporter system for screening affinity peptides, JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, Vol: 70, Pages: 435-439, ISSN: 0165-022X

Journal article

Edel JB, Lahoud P, Cass AEG, deMello AJet al., 2007, Discrimination between single Escherichia coli cells using time-resolved confocal spectroscopy, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 111, Pages: 1129-1134, ISSN: 1520-6106

Journal article

Yue X, Drakakis EA, Ye H, Lim M, Mantalaris A, Panoskaltsis N, Radomska A, Toumazou C, Cass Tet al., 2007, An on-line, multi-parametric, multi-channel physicochemical monitoring platform for stem cell culture bioprocessing, IEEE International Symposium on Circuits and Systems, Publisher: IEEE, Pages: 1215-+, ISSN: 0271-4302

Conference paper

Yue X, Drakakis EM, Hua Y, Mayasari L, Mantalaris A, Panoskaltsis N, Radomska A, Toumazou C, Cass AEGet al., 2007, An On-line, Multi-Parametric, Multi-Channel Physicochemical Monitoring Platform for Stem Cell Culture Bioprocessing, IEEE International Symposium on Circuits and Systems (ISCAS), Publisher: IEEE, Pages: 1215-1218

Conference paper

Huang X, Zhang X-E, Zhou Y-F, Zhang Z-P, Cass AEGet al., 2006, Directed evolution of the 5'-untranslated region of the phoA gene in Escherichia coli simultaneously yields a stronger promoter and a stronger Shine-Dalgarno sequence., Biotechnol J, Vol: 1, Pages: 1275-1282

Directed evolution has been widely applied for gene improvement through random mutagenesis of coding sequences. Through error-prone PCR both in the coding sequence and the regulatory sequence of E. coli alkaline phosphatase, the cellular enzyme activity has been efficiently enhanced. Sequence analysis revealed that the resultant mutant 34-B12, which showed a sevenfold increased enzyme activity at the cellular level, contains three mutations in the regulatory sequence and another three mutations in the coding sequence. Activity assays of the enzyme containing the corresponding amino acid substitutions proved that the amino acid mutations contribute only to a small portion to the increased cellular enzyme activity. So the mutations in the 5'-untranslated region were analyzed separately and combinationally. The results suggested that one mutation yielded a stronger promoter and the other two mutations both elevated the E. coli alkaline phosphatase expression at the translational level; moreover, a stronger Shine-Dalgarno sequence was generated.

Journal article

Liu FM, Kollensperger PA, Green M, Cass AEG, Cohen LFet al., 2006, A note on distance dependence in surface enhanced Raman spectroscopy, CHEMICAL PHYSICS LETTERS, Vol: 430, Pages: 173-176, ISSN: 0009-2614

Journal article

Topoglidis E, Campbell CJ, Cass AEG, Durrant JRet al., 2006, Nitric oxide biosensors based on the immobilization of hemoglobin on mesoporous titania electrodes, ELECTROANALYSIS, Vol: 18, Pages: 882-887, ISSN: 1040-0397

Journal article

Zhang JK, Cass AEG, 2006, Kinetic study of site directed and randomly immobilized his-tag alkaline phosphatase by flow injection chemiluminescence, JOURNAL OF MOLECULAR RECOGNITION, Vol: 19, Pages: 243-246, ISSN: 0952-3499

Journal article

Bedford C, Cas T, Francois I, Harding Set al., 2006, Glycomics: From glycobiology to diagnostics and therapeutics., Pages: 163-172, ISSN: 0214-0934

The Royal Society of Chemistry Biotechnology Group and Chemical Biology Forum held a two-day symposium on December 12-13, 2005, in London. The meeting was designed to give an overview of the exciting new technologies being applied to study complex carbohydrates from their sequence analysis, characterization and function through to the development of novel pharmacological approaches to diagnose and alleviate polysaccharide-mediated diseases. The meeting, which also included a poster session, highlighted the multidisciplinary nature of the research and development and the exciting advances being made in this field.

Conference paper

Taylor PM, Cass AEG, Yacoub MH, 2006, Extracellular Matrix Scaffolds for Tissue Engineering Heart Valves., Progress in Pediatric Cardiology, Vol: 21, Pages: 219-225, ISSN: 1058-9813

Journal article

Yue X, Drakakis EM, Toumazou C, Ye H, Mantalaris A, Radomska A, Cass AEGet al., 2006, 8x16 Channel Physiological Monitoring Platform of Stem Cell Culture Systems, IEEE Biomedical Circuits and Systems Conference (BioCAS), Publisher: IEEE, Pages: 106-109

Conference paper

Green M, Liu F-M, Cohen L, Kollensperger P, Cass Tet al., 2006, SERS platforms for high density DNA arrays, Faraday Discuss., Vol: 132, Pages: 269-280-269-280

Surface Enhanced Raman Scattering (SERS) gives rise to analytical applications with much promise. In our approach three steps are necessary. We require a SERS platform of high enhancement. This has been achieved using the special technique of Island Lithography, combined with Ag deposition by galvanic exchange, yielding an enhancement factor of 10. Probe oligonucleotide molecules are attached to a specific area on the platform, at the optimized surface concentration, using thiolated single stranded (ss) DNA molecules. The optimum surface concentration has been determined and interpreted in the light of the polyelectrolyte behaviour of ssDNA. Finally the change in SERS produced by hybridisation of the probe molecules to a target DNA molecule is measured. Highly discernible changes have been obtained. No change in probe signal is seen when presented with one base mismatched target. From this work it is concluded that the prospects for label-free DNA detection in high-density arrays is now close to achievement.

Journal article

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