267 results found
Winkle RF, Nagy JM, Cass AEG, et al., 2008, Towards microfluidic technology-based MALDI-MS platforms for drug discovery: a review, EXPERT OPINION ON DRUG DISCOVERY, Vol: 3, Pages: 1281-1292, ISSN: 1746-0441
Nagy J, Kang Y, Cass A, et al., 2008, Finding proteins in cell-conditioned media, Genetic Engineering and Biotechnology News, Vol: 28, ISSN: 1935-472X
Pereira F, Hassard S, Cass A, et al., 2008, CZE of peptides using a multi-pixel detector array, Chimica Oggi, Vol: 26, Pages: 40-42, ISSN: 0392-839X
Capillary electrophoresis has been used in the analysis of peptides for decades, however traditional methods show excessive variability due to composition and sequence of the peptide, and variation in the interaction of the peptide with the separation medium. Multi-pixel detection along with the associated data analysis software improves reproducibility and sensitivity beyond current methods using UV absorption detection. The reproducibility of the data and the sensitivity of this 'no-label' multi-pixel detection approach are investigated using a mixture of known peptides. In the near future this will enable peptide mass finger printing based on the ability to assign molecular weight values to the peptides separated by charge.
Pereira F, Hassard S, Cass A, et al., 2008, CZE of peptides using a multi-pixel detector array, CHIMICA OGGI-CHEMISTRY TODAY, Vol: 26, Pages: 40-42, ISSN: 0392-839X
Lim M, Ye H, Drakakis EM, et al., 2008, Towards information-rich bioprocessing: Generation of spatio-temporal profiles through the use of design of experiments to determine optimal number and location of sensors - An example in thermal profiles, Biochemical Engineering Journal, Vol: 40, Pages: 1-7, ISSN: 1369-703X
New SEP, Chester AH, Sharma S, et al., 2008, The effect of carbon nanotubes on mesenchymal stem cells in a 3D environment, Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, Publisher: MARY ANN LIEBERT INC, Pages: 912-912, ISSN: 1937-3341
Patel BA, Arundell M, Quek RGW, et al., 2008, Individually addressable microelectrode array for monitoring oxygen and nitric oxide release, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol: 390, Pages: 1379-1387, ISSN: 1618-2642
White SL, Chadban SJ, Jan S, et al., 2008, How can we achieve global equity in provision of renal replacement therapy?, Bull World Health Organ, Vol: 86, Pages: 229-237
There is a significant emerging burden of chronic and end-stage kidney disease in low- and middle-income countries, driven by population ageing and the global epidemic of type 2 diabetes. Sufferers of end-stage kidney disease require ongoing dialysis or kidney transplantation to survive; however, in many low- and middle-income countries, treatment options are strictly limited or unaffordable. Low numbers of maintenance dialysis patients and transplant recipients reflect profound economic and service provision challenges for health-care systems in low- and middle-income countries in sustaining renal replacement therapy programmes. Underdeveloped organ donor and transplant programmes, health system and financing issues, ethical regulation of transplantation and the cost of pharmaceuticals commonly pose additional barriers to the delivery of efficient and cost-effective renal replacement therapy. Development of locally appropriate transplant programmes, effective use of nongovernmental sources of funding, service planning and cost containment, use of generic drugs and local manufacture of dialysis consumables have the potential to make life-saving renal replacement therapy available to many more in need. Select low- and middle-income countries demonstrate more equitable provision of renal replacement therapy is possible outside high-income countries. For other low- and middle-income countries, education, the development of good public policy and a supportive international environment are critical. Prevention of end-stage kidney disease, ideally as part of an integrated approach to chronic vascular diseases, must also be a key objective.
Johnson CJ, Zhukovsky N, Cass AEG, et al., 2008, Proteomics, nanotechnology and molecular diagnostics, PROTEOMICS, Vol: 8, Pages: 715-730, ISSN: 1615-9853
Cass T, 2008, Biosensors, Advances in Tissue Engineering, Pages: 373-399, ISBN: 9781848161825
© 2008 by Imperial College Press. Increasingly biological sciences are being built on a quantitative foundation, based upon our ability to accurately determine the temporal and spatial variations in the concentration of key molecules. It is this quantitative analytical data that provides the testable basis for building hypotheses about biological systems. Although many, diverse analytical techniques have been used to collect quantitative data these are often destructive of the system being analysed. Biosensors by contrast promise the ability to measure selected molecules continuously and in real-time with good spatial resolution. They use specific molecular recognition at the surface of a transducer such that an electrical signal is generated in proportion to the concentration of the target analyte. Many different molecular recognition reagents have been exploited in this respect and include enzymes, binding proteins and nucleic acids, whilst electrochemical, optical and mass sensitive signal transduction devices have been used to generate the electrical signal (typically a voltage or current). Using biosensors in vivo presents additional challenges over and above simply relating signal to concentration. Biocompatibility is an ever-present issue and includes both the effect of the biological matrix on the sensor as well as sensor components on the biology. In this chapter a review of the different sensing modes is presented and their potential applicability to the monitoring of cells, tissue and tissue constructs is presented.
Radomska A, Singhal S, Ye H, et al., 2008, Biocompatible ion selective electrode for monitoring metabolic activity during the growth and cultivation of human cells, Biosensors and Bioelectronics, Vol: 24, Pages: 435-441, ISSN: 1873-4235
Yue X, Drakakis EM, Lim M, et al., 2008, A Real-Time Multi-Channel Monitoring System for Stem Cell Culture Process, IEEE Transactions on Biomedical Circuits and Systems, Vol: 2, Pages: 66-77, ISSN: 1932-4545
Hassard J, Cass T, 2007, High-throughput monitoring of protein folding, Innovations in Pharmaceutical Technology, ISSN: 1471-7204
A novel technology has been developed for the rapid and accurate analysis of the folded state of proteins, enabling protein folding to be monitored on a high-throughput basis for drug discovery and manufacturing processes.
Cass AEG, 2007, Organometallic compounds in biosensing, Comprehensive Organometallic Chemistry III, Pages: 589-602, ISBN: 9780080450476
Toumazou C, Cass T, 2007, Cell-bionics: tools for real-time sensor processing, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 362, Pages: 1321-1328, ISSN: 0962-8436
Cass AEG, 2007, Building Biolelectronic Interfaces, Electronics Letters, Vol: 43, Pages: 903-905
Lim M, Ye H, Panoskaltsis N, et al., 2007, Intelligent Bioprocessing for Haemotopoietic Cell Cultures Using Monitoring and Design of Experiments, Biotechnology Advances, Vol: 25, Pages: 353-368, ISSN: 0734-9750
Cass A, Cunningham J, Anderson K, et al., 2007, Decision-making about suitability for kidney transplantation: Results of a national survey of Australian nephrologists., Nephrology (Carlton), Vol: 12, Pages: 299-304, ISSN: 1320-5358
AIM: This study aimed to elucidate the factors affecting nephrologists' decision-making on patients' suitability for kidney transplantation. Given the reduced access to transplantation for Indigenous Australians, the role of patient's ethnicity was of particular interest. METHODS: A postal survey of practising nephrologists and trainees was undertaken in Australia. Each participant was provided with a unique set of 15 hypothetical patient descriptions, with demographic, clinical and behavioural factors randomly generated to ensure an overall balance of factors across the cases. The main outcome measure was whether kidney transplantation was recommended. RESULTS: Responding nephrologists and trainees were more likely to recommend transplantation for hypothetical patients who were young, of normal weight and described as compliant. They were less likely to recommend transplantation for smokers, or for people with diabetes or heart disease. No significant differences related to the patients' sex or ethnicity. The geographical location of the respondent was a significant determinant, with differences according to their State/Territory and their metropolitan/non-metropolitan location. CONCLUSION: When all other factors were held constant, nephrologists and trainees appear to base their decision-making regarding suitability for transplant on clinical and behavioural factors, rather than on the basis of ethnicity or sex. In practice, however, clinical and behavioural factors cluster with ethnicity, and this is likely to contribute to the current poor access to transplantation for Indigenous end-stage kidney disease patients. Apparent differences in decision-making according to the respondent's location may reflect variations in practice across the country.
Huang X, Zhang X-E, Zhou Y-F, et al., 2007, Construction of a high sensitive Escherichia coli alkaline phosphatase reporter system for screening affinity peptides, JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, Vol: 70, Pages: 435-439, ISSN: 0165-022X
Edel JB, Lahoud P, Cass AEG, et al., 2007, Discrimination between single Escherichia coli cells using time-resolved confocal spectroscopy, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 111, Pages: 1129-1134, ISSN: 1520-6106
Yue X, Drakakis EA, Ye H, et al., 2007, An on-line, multi-parametric, multi-channel physicochemical monitoring platform for stem cell culture bioprocessing, IEEE International Symposium on Circuits and Systems, Publisher: IEEE, Pages: 1215-+, ISSN: 0271-4302
Yue X, Drakakis EM, Hua Y, et al., 2007, An On-line, Multi-Parametric, Multi-Channel Physicochemical Monitoring Platform for Stem Cell Culture Bioprocessing, IEEE International Symposium on Circuits and Systems (ISCAS), Publisher: IEEE, Pages: 1215-1218
Huang X, Zhang X-E, Zhou Y-F, et al., 2006, Directed evolution of the 5'-untranslated region of the phoA gene in Escherichia coli simultaneously yields a stronger promoter and a stronger Shine-Dalgarno sequence., Biotechnol J, Vol: 1, Pages: 1275-1282
Directed evolution has been widely applied for gene improvement through random mutagenesis of coding sequences. Through error-prone PCR both in the coding sequence and the regulatory sequence of E. coli alkaline phosphatase, the cellular enzyme activity has been efficiently enhanced. Sequence analysis revealed that the resultant mutant 34-B12, which showed a sevenfold increased enzyme activity at the cellular level, contains three mutations in the regulatory sequence and another three mutations in the coding sequence. Activity assays of the enzyme containing the corresponding amino acid substitutions proved that the amino acid mutations contribute only to a small portion to the increased cellular enzyme activity. So the mutations in the 5'-untranslated region were analyzed separately and combinationally. The results suggested that one mutation yielded a stronger promoter and the other two mutations both elevated the E. coli alkaline phosphatase expression at the translational level; moreover, a stronger Shine-Dalgarno sequence was generated.
Liu FM, Kollensperger PA, Green M, et al., 2006, A note on distance dependence in surface enhanced Raman spectroscopy, CHEMICAL PHYSICS LETTERS, Vol: 430, Pages: 173-176, ISSN: 0009-2614
Topoglidis E, Campbell CJ, Cass AEG, et al., 2006, Nitric oxide biosensors based on the immobilization of hemoglobin on mesoporous titania electrodes, ELECTROANALYSIS, Vol: 18, Pages: 882-887, ISSN: 1040-0397
Zhang JK, Cass AEG, 2006, Kinetic study of site directed and randomly immobilized his-tag alkaline phosphatase by flow injection chemiluminescence, JOURNAL OF MOLECULAR RECOGNITION, Vol: 19, Pages: 243-246, ISSN: 0952-3499
Bedford C, Cas T, Francois I, et al., 2006, Glycomics: From glycobiology to diagnostics and therapeutics., Pages: 163-172, ISSN: 0214-0934
The Royal Society of Chemistry Biotechnology Group and Chemical Biology Forum held a two-day symposium on December 12-13, 2005, in London. The meeting was designed to give an overview of the exciting new technologies being applied to study complex carbohydrates from their sequence analysis, characterization and function through to the development of novel pharmacological approaches to diagnose and alleviate polysaccharide-mediated diseases. The meeting, which also included a poster session, highlighted the multidisciplinary nature of the research and development and the exciting advances being made in this field.
Taylor PM, Cass AEG, Yacoub MH, 2006, Extracellular Matrix Scaffolds for Tissue Engineering Heart Valves., Progress in Pediatric Cardiology, Vol: 21, Pages: 219-225, ISSN: 1058-9813
Yue X, Drakakis EM, Toumazou C, et al., 2006, 8x16 Channel Physiological Monitoring Platform of Stem Cell Culture Systems, IEEE Biomedical Circuits and Systems Conference (BioCAS), Publisher: IEEE, Pages: 106-109
Surface Enhanced Raman Scattering (SERS) gives rise to analytical applications with much promise. In our approach three steps are necessary. We require a SERS platform of high enhancement. This has been achieved using the special technique of Island Lithography, combined with Ag deposition by galvanic exchange, yielding an enhancement factor of 10. Probe oligonucleotide molecules are attached to a specific area on the platform, at the optimized surface concentration, using thiolated single stranded (ss) DNA molecules. The optimum surface concentration has been determined and interpreted in the light of the polyelectrolyte behaviour of ssDNA. Finally the change in SERS produced by hybridisation of the probe molecules to a target DNA molecule is measured. Highly discernible changes have been obtained. No change in probe signal is seen when presented with one base mismatched target. From this work it is concluded that the prospects for label-free DNA detection in high-density arrays is now close to achievement.
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